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黄龙病对柑橘叶际微生物组的影响

引用本文：黄峰,崔一平,宋晓兵,彭埃天,凌金锋,陈霞.黄龙病对柑橘叶际微生物组的影响.植物保护学报,2023,50(5):1150-1160

DOI：10.13802/j.cnki.zwbhxb.2023.2023806

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作者	单位	E-mail
黄峰	广东省农业科学院植物保护研究所, 农业农村部华南果蔬绿色防控重点实验室, 广东省植物保护新技术重点实验室, 广州 510640
崔一平	广东省农业科学院植物保护研究所, 农业农村部华南果蔬绿色防控重点实验室, 广东省植物保护新技术重点实验室, 广州 510640
宋晓兵	广东省农业科学院植物保护研究所, 农业农村部华南果蔬绿色防控重点实验室, 广东省植物保护新技术重点实验室, 广州 510640	xbsong@126.com
彭埃天	广东省农业科学院植物保护研究所, 农业农村部华南果蔬绿色防控重点实验室, 广东省植物保护新技术重点实验室, 广州 510640
凌金锋	广东省农业科学院植物保护研究所, 农业农村部华南果蔬绿色防控重点实验室, 广东省植物保护新技术重点实验室, 广州 510640
陈霞	广东省农业科学院植物保护研究所, 农业农村部华南果蔬绿色防控重点实验室, 广东省植物保护新技术重点实验室, 广州 510640

中文摘要:为明确柑橘叶际微生物组对黄龙病发生的响应规律，采用荧光定量PCR技术检测采集柑橘叶片感染黄龙病菌的情况，通过扩增子测序分析法对PCR检测结果为阴性和阳性叶片的微生物群落进行比较，并通过相关性分析和网络分析对黄龙病菌和叶际微生物的相关性进行解析。结果显示，夏季采集的柑橘叶片中黄龙病菌的检出率为7.5%，而到秋季黄龙病菌的检出率上升到32.3%。黄龙病的发生显著改变了柑橘叶际细菌群落的组成和结构，对于细菌类群的丰度也有一定影响；而对叶际真菌群落的组成和结构无显著影响。黄龙病菌与叶际细菌存在普遍的负相关关系，正相关关系相对较少但相关性更强。黄龙病菌可能通过与厌氧棍状菌Anaerotruncus sp.、梭菌Clostridiales sp.、假单胞菌Pseudomonas sp.、鞘脂单胞菌Sphingomonas sp.、毛螺菌Lachnospiraceae sp.和链球菌Streptococcus sp.的负向互作对柑橘叶际整个细菌群落产生负影响。表明黄龙病发生对柑橘叶际真菌和细菌群落的影响规律不同，同时黄龙病菌可能通过与几种主要细菌的负向互作来实现对叶际微生物组的影响。

中文关键词:柑橘黄龙病叶际微生物组细菌真菌群落组成丰度

The influences of citrus huanglongbing on phyllosphere microbiome

Author Name	Affiliation	E-mail
Huang Feng	Guangdong Provincial Key Laboratory of High Technology for Plant Protection Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong Province, China
Cui Yiping	Guangdong Provincial Key Laboratory of High Technology for Plant Protection Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong Province, China
Song Xiaobing	Guangdong Provincial Key Laboratory of High Technology for Plant Protection Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong Province, China	xbsong@126.com
Peng Aitian	Guangdong Provincial Key Laboratory of High Technology for Plant Protection Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong Province, China
Ling Jinfeng	Guangdong Provincial Key Laboratory of High Technology for Plant Protection Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong Province, China
Chen Xia	Guangdong Provincial Key Laboratory of High Technology for Plant Protection Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong Province, China

Abstract:To determine the influences of citrus huanglongbing to leaf microbiome, citrus leaves were broadly collected and detected without/with the causal agent of huanglongbing with quantitative PCR method. In combination with fungal and bacterial amplicon sequencing, the phyllosphere microbiome of the selected huanglongbing-negative and -positive leaves were compared, and the association between the causal agent of huanglongbing and different microbial groups were analyzed based on the correlation and network analysis. The results showed an obvious increase of the average huanglongbing incidence from 7.5% in summer to 32.3% in autumn in sampled orchards. The occurrence of citrus huanglongbing significantly altered the composition and structure of the phyllosphere bacterial communities, and had a relative strong influence on the relative abundances of bacterial taxa. In comparison, huanglongbing had a minor effect on the phyllosphere fungal communities. Besides, the causal agent of huanglongbing showed general negative correlation to the phyllosphere bacteria, while the positive correlation was less but more significant. The causal agent of huanglongbing might convey its negative influences to the whole bacterial community through its negative interactions with multiple bacterial species (Anaerotruncus sp., Clostridiales sp., Pseudomonas sp., Sphingomonas sp., Lachnospiraceae sp. and Streptococcus sp.). This study indicated the different influences of huanglongbing to the phyllosphere fungal and bacterial communities, and the influences of huanglongbing might be achieved by the negative interactions between its causal agent and multiple phyllosphere bacteria.

keywords:citrus huanglongbing phyllosphere microbiome bacteria fungi community composition abundance

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