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黄芩苷对稻瘟病菌细胞生长发育的影响及其对稻瘟病的防效

引用本文：梁美玲,封金奇,汪文娟,杨健源,朱小源,苏菁.黄芩苷对稻瘟病菌细胞生长发育的影响及其对稻瘟病的防效.植物保护学报,2023,50(5):1176-1183

DOI：10.13802/j.cnki.zwbhxb.2023.2023808

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作者	单位	E-mail
梁美玲	广东省农业科学院植物保护研究所, 广东省植物保护新技术重点实验室, 广州 510640
封金奇	广东省农业科学院植物保护研究所, 广东省植物保护新技术重点实验室, 广州 510640
汪文娟	广东省农业科学院植物保护研究所, 广东省植物保护新技术重点实验室, 广州 510640
杨健源	广东省农业科学院植物保护研究所, 广东省植物保护新技术重点实验室, 广州 510640
朱小源	广东省农业科学院植物保护研究所, 广东省植物保护新技术重点实验室, 广州 510640
苏菁	广东省农业科学院植物保护研究所, 广东省植物保护新技术重点实验室, 广州 510640	1835795725@qq.com

中文摘要:为研制新型绿色高效稻瘟病防控药剂，分别测定黄芩苷对稻瘟病菌Magnaporthe oryzae附着胞形成、菌落生长、菌丝侵染和致病力的影响以及对稻瘟病的防效。结果显示，黄芩苷抑制稻瘟病菌附着胞形成和菌落生长的最低浓度分别为100 μmol/L和500 μmol/L，在100 μmol/L浓度下黄芩苷能抑制稻瘟病菌侵染菌丝在水稻叶鞘细胞中的扩展及叶片病斑的形成；在盆栽接种试验中，对照组的水稻苗表现为感病和中抗，所占比例分别为38%和62%，而黄芩苷处理组的水稻苗表现为抗病和中抗，其中表现为抗病的水稻苗比例超过70%；黄芩苷处理后，稻瘟病菌分生孢子细胞死亡率比对照组极显著降低。表明黄芩苷通过抑制稻瘟病菌分生孢子的细胞程序性死亡干扰了稻瘟病菌附着胞的形成，从而能有效防控稻瘟病，具有开发为新型绿色高效稻瘟病防控药剂的潜力。

中文关键词:稻瘟病菌黄芩苷附着胞细胞程序性死亡防效

Baicalin affects the cell development of Magnaporthe oryzae and its control efficacy on rice blast

Author Name	Affiliation	E-mail
Liang Meiling	Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong Province, China
Feng Jinqi	Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong Province, China
Wang Wenjuan	Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong Province, China
Yang Jianyuan	Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong Province, China
Zhu Xiaoyuan	Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong Province, China
Su Jing	Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong Province, China	1835795725@qq.com

Abstract:In order to develop a new green and efficient control agent on rice blast, the effects of baicalin on Magnaporthe oryzae appressorium formation, colony growth, hyphal infection, the pathogenicity to rice and the control efficacy on rice blast were tested. The results showed that the lowest concentration of baicalin inhibiting appressorium formation and colony growth in M. oryzae were 100 μmol/L and 500 μmol/L, respectively. At the concentration of 100 μmol/L baicalin, the expansion of M. oryzae infection hypha in rice leaf sheath cells and the formation of lesion on rice leaves were inhibited. In pot inoculation, the resistance levels of control rice seedlings (CK) were susceptible and moderately resistant, with the proportions of 38% and 62% respectively, while the resistance levels of rice seedlings with baicalin treatment group were resistant or moderately resistant, more than 70% seedlings showed resistance. Futhermore, the conidial cell death rate with the treatment of baicalin was significantly lower than the control group. The study showed that baicalin interfered with the appressorium formation of M. oryzae by inhibiting conidial programmed cell death, and significantly controlled rice blast. Therefore, baicalin has the potential to be developed as a novel green and efficient control agent on rice blast.

keywords:Magnaporthe oryzae baicalin appressorium programmed cell death control efficacy

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