| 珠江三角洲地区黄毛鼠抗药性的发生趋势及其Vkorc1基因多态性 |
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| 引用本文：姚丹丹,姜洪雪,林思亮,秦姣,冯志勇.珠江三角洲地区黄毛鼠抗药性的发生趋势及其Vkorc1基因多态性.植物保护学报,2023,50(5):1202-1209 |
| DOI：10.13802/j.cnki.zwbhxb.2023.2023811 |
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| 作者 | 单位 | E-mail | | 姚丹丹 | 广东省农业科学院植物保护研究所, 农业农村部华南果蔬绿色防控重点实验室, 广东省植物保护新技术重点实验室, 广州 510640 | | | 姜洪雪 | 广东省农业科学院植物保护研究所, 农业农村部华南果蔬绿色防控重点实验室, 广东省植物保护新技术重点实验室, 广州 510640 | | | 林思亮 | 广东省农业科学院植物保护研究所, 农业农村部华南果蔬绿色防控重点实验室, 广东省植物保护新技术重点实验室, 广州 510640 | | | 秦姣 | 广东省农业科学院植物保护研究所, 农业农村部华南果蔬绿色防控重点实验室, 广东省植物保护新技术重点实验室, 广州 510640 | qjohy@163.com | | 冯志勇 | 广东省农业科学院植物保护研究所, 农业农村部华南果蔬绿色防控重点实验室, 广东省植物保护新技术重点实验室, 广州 510640 | 13318854585@163.com |
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| 中文摘要:为阐明珠江三角洲地区黄毛鼠Rattus losea对第1代抗凝血灭鼠剂的抗性发生趋势及其遗传机制，以杀鼠灵为标准药物，采用致死期食毒法对2017—2021年在广东省江门市捕获的165只黄毛鼠进行生理抗性检测，并测定每只试鼠的维生素K环氧化物还原酶复合物亚单位1（vitamin Kepoxide reductase complex subunit 1，Vkorc1）的编码基因序列，分析其突变情况。结果显示，江门市黄毛鼠对第1代抗凝血灭鼠剂杀鼠灵的抗性率为27.03%~50.00%，在黄毛鼠Vkorc1基因中检测到6个不同的突变位点，包括2个错义突变位点Arg58Gly及Tyr139Cys和4个沉默突变位点Ala41Ala、Cys96Cys、Arg98Arg及Ala143Ala，突变率分别为87.27%、0.61%、1.21%、0.61%、1.21%和0.61%，其中Ala143Ala是在黄毛鼠中新发现的沉默突变位点。表明珠江三角洲地区黄毛鼠已对第1代抗凝血灭鼠剂产生了群体抗性并呈上升趋势，第58位的精氨酸突变成甘氨酸（Arg58Gly）是黄毛鼠抗性基因Vkorc1的主要突变位点。 |
| 中文关键词:珠江三角洲 黄毛鼠 抗性 抗凝血灭鼠剂 Vkorc1基因 杀鼠灵 |
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| Occurrence trend of rodenticide resistance and Vkorc1 gene polymorphism in lesser ricefield rat Rattus losea in the Pearl River Delta |
| Author Name | Affiliation | E-mail | | Yao Dandan | Guangdong Provincial Key Laboratory of High Technology for Plant Protection
Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs
Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong Province, China | | | Jiang Hongxue | Guangdong Provincial Key Laboratory of High Technology for Plant Protection
Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs
Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong Province, China | | | Lin Siliang | Guangdong Provincial Key Laboratory of High Technology for Plant Protection
Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs
Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong Province, China | | | Qin Jiao | Guangdong Provincial Key Laboratory of High Technology for Plant Protection
Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs
Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong Province, China | qjohy@163.com | | Feng Zhiyong | Guangdong Provincial Key Laboratory of High Technology for Plant Protection
Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs
Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong Province, China | 13318854585@163.com |
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| Abstract:To elucidate the occurrence trend of the resistance of lesser ricefield rat Rattus losea in the Pearl River Delta to the first-generation anticoagulant rodenticide and its genetic mechanism, by using warfarin as the standard drug, the physiological resistance of 165 R. losea captured in Jiangmen City, Guangdong Province from 2017 to 2021 was detected using the lethal feeding period method. In addition, the gene sequence of vitamin K epoxide reductase complex subunit 1 (Vkorc1) was determined for each rat tested, and the mutation status of Vkorc1 gene was analyzed. The results showed that the resistance rate of R. losea in Jiangmen City to the first-generation anticoagulant rodenticide ranged from 27.03% to 50.00%. Six different mutation sites were detected in the Vkorc1 gene of R. losea, including two missense mutations, Arg58Gly and Tyr139Cys, and four silent mutations, Ala41Ala, Cys96Cys, Arg98Arg, and Ala143Ala, with a mutation rate of 87.27%, 0.61%, 1.21%, 0.61%, 1.21% and 0.61%, respectively. Among them, Ala143Ala was a newly discovered silent mutation in R. losea. These results indicated that R. losea in the Pearl River Delta has developed population resistance to the first-generation anticoagulant rodenticide with an increasing trend. The arginine at position 58 mutated to glycine (Arg58Gly) was the main mutation site of the resistance gene Vkorc1 in R. losea. |
| keywords:Pearl River Delta Rattus losea resistance anticoagulant rodenticide Vkorc1 gene warfarin |
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