| 星豹蛛NPC2基因家族克隆及表达模式分析 |
| 点此下载全文 |
| 引用本文：赵萌萌,焦丽亚琳,牛越,王美,张晓晨,李锐.星豹蛛NPC2基因家族克隆及表达模式分析.植物保护学报,2023,50(5):1289-1296 |
| DOI：10.13802/j.cnki.zwbhxb.2023.2022089 |
| 摘要点击次数: |
| 全文下载次数: |
|
|
| 中文摘要:为探究气味载体蛋白——尼曼匹克C2型蛋白（Niemann-Pick type C2 protein，NPC2）在星豹蛛Pardosa astrigera体内的功能，从星豹蛛体内筛选鉴定出PaNPC2-1和PaNPC2-2基因并采用反转录聚合酶链式反应（reverse transcription-polymerase chain reaction，RT-PCR）技术进行基因克隆，利用实时荧光定量PCR（quantitative real-time PCR，RT-qPCR）技术对星豹蛛不同组织、不同发育期PaNPC2-1和PaNPC2-2基因的表达模式进行分析。结果显示，PaNPC2-1和PaNPC2-2开放阅读框长度分别为441 bp和492 bp，编码146个和163个氨基酸。序列分析显示，2个PaNPC2基因具有NPC2基因家族的典型特征，且具有6个保守的半胱氨酸位点。组织表达谱结果显示，PaNPC2-1在雌成蛛触肢以及雄成蛛螯肢中的表达量最高，PaNPC2-2在雌成蛛躯干的表达量显著高于雌成蛛的其他组织；发育期表达谱结果显示，PaNPC2-1在6龄幼蛛期的表达量最高，PaNPC2-2在成蛛期的表达量最高。推测PaNPC2-1可能参与星豹蛛的化学通信，而PaNPC2-2则行使着不同于化学通信的其他功能。 |
| 中文关键词:星豹蛛 NPC2蛋白 基因克隆 表达分析 化学通信 |
| |
| Cloning and expression pattern analysis of NPC2 gene family in a wolf spider Pardosa astrigera (Araneae: Lycosidae) |
| Author Name | Affiliation | E-mail | | Zhao Mengmeng | College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China | | | Jiao Liyalin | College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China | | | Niu Yue | College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China | | | Wang Mei | College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China | | | Zhang Xiaochen | College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China | | | Li Rui | College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China | sxaulr@126.com |
|
|
| Abstract:To investigate the function of the odor-carrier protein Niemann-Pick type C2 protein (NPC2) in wolf spider Pardosa astrigera, PaNPC2-1 and PaNPC2-2 genes were cloned by using reverse transcription-polymerase chain reaction (RT-PCR). The expression patterns of PaNPC2-1 and PaNPC2-2 genes in different tissues and at different developmental stages of P. astrigera were analyzed using quantitative reverse transcription PCR (RT-qPCR). The results showed that the length of open reading frames of PaNPC2-1 and PaNPC2-2 were 441 bp and 492 bp, encoding 146 and 163 amino acids, respectively. Sequence analysis showed that the two PaNPC2 genes had the typical characteristics of NPC2 family genes, with six conserved cysteine residues. Tissue expression profile showed that PaNPC2-1 expression level was highest in the pedipalps of female adults and the chelicerae of male adults; PaNPC2-2 expression level in the cephalothorax and abdomen of female adults was significantly higher than that in other tissues of male and female adults. The expression profile at different developmental stages indicated that the expression level of PaNPC2-1 was the highest in six-instar juvenile spiders and that of PaNPC2-2 was the highest in adult spiders By analyzing the spatio-temporal expression profiles, it is speculated that PaNPC2-1 may be involved in chemical communication in P. astrigera, while PaNPC2-2 not. |
| keywords:Pardosa astrigera NPC2 protein gene cloning expression analysis chemical communication |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
|
您是本站第 9457031 版权所有：植物保护学报 京ICP备05006550号-2 主管单位：中国科协 主办单位：中国植物保护学会、中国农业大学 地址：北京市圆明园西路2号 中国农业大学植物保护学院 植物保护学报编辑部 电话：010-62732528 电子邮件：zbxb@cau.edu.cn 技术支持： 北京勤云科技发展有限公司 |
