| 苹果茎沟病毒柑橘碎叶株系的分布与序列分析 |
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| 引用本文：高海馨,陈香玲,乔兴华,廖慧红,周常勇,周彦.苹果茎沟病毒柑橘碎叶株系的分布与序列分析.植物保护学报,2023,50(5):1318-1326 |
| DOI：10.13802/j.cnki.zwbhxb.2023.2022080 |
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| 中文摘要:为明确苹果茎沟病毒（apple stem grooving virus，ASGV）柑橘碎叶株系（citrus tatter leaf virus，CTLV）在中国柑橘产区的发生分布及其分子特性，于2017—2021年对我国12个柑橘主产区开展系统调查，并采用多重比对以及系统发育分析法对CTLV的全序列进行分析。结果表明，从12个柑橘主产区采集的2 012份疑似样品中检测出413份感CTLV阳性样品。除陕西省外，其余11个柑橘主产区样品中均检测出CTLV，并且柚类样品中CTLV的检出率最高，达到32.31%。对分离获得的22株CTLV毒株和已知的7株CTLV以及5株ASGV毒株进行全序列分析，发现所有34株毒株的核苷酸序列相似性为78.6%~99.8%，且CTLV序列的保守性较高。此外，与其他4株ASGV毒株相比，本研究中22株CTLV毒株与ASGV-MK毒株（GenBank登录号为MZ127820.1）具有更近的亲缘关系。推测CTLV中国毒株间的亲缘关系可能与其采样地和寄主品种有关。 |
| 中文关键词:苹果茎沟病毒 柑橘碎叶株系 检测 序列分析 系统进化分析 |
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| Distribution and molecular characterization of citrus tatter leaf virus in China |
| Author Name | Affiliation | E-mail | | Gao Haixin | Integrative Science Center of Germplasm Creation in Western China(Chongqing) Science City, Chongqing 400000, China
National Citrus Engineering Research Center, Citrus Research Institute, Southwest University, Chongqing 400712, China | | | Chen Xiangling | Horticultural Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, Guangxi Zhuang Autonomous Region, China | | | Qiao Xinghua | Plant Protection and Fruit Tree Technology Popularization Station in Wanzhou District of Chongqing City, Wanzhou 404199, Chongqing, China | | | Liao Huihong | Horticultural Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, Guangxi Zhuang Autonomous Region, China | | | Zhou Changyong | Integrative Science Center of Germplasm Creation in Western China(Chongqing) Science City, Chongqing 400000, China
National Citrus Engineering Research Center, Citrus Research Institute, Southwest University, Chongqing 400712, China | zhoucy@cric.cn | | Zhou Yan | Integrative Science Center of Germplasm Creation in Western China(Chongqing) Science City, Chongqing 400000, China
National Citrus Engineering Research Center, Citrus Research Institute, Southwest University, Chongqing 400712, China | zhouyan@cric.cn |
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| Abstract:To better understand the distribution and molecular characteristics of citrus tatter leaf virus (CTLV) in China, a field survey was conducted from 2017 to 2021, and a total of 2 012 citrus samples were collected from 12 Chinese major citrus-growing provinces. Among these, 413 citrus samples were tested positive for CTLV, and this virus was present in all of the Chinese major citrus-growing provinces, except Shaanxi Province. In surveyed citrus genotypes, the most frequently infected genotype was pummelo, with an infection rate of 32.31%. CTLV is a strain of apple stem grooving virus (ASGV, Capillovirus). In this study, the complete genome sequences of 22 CTLV isolates from different provinces and hosts were sequenced. Comparisons of the whole-genome sequences of these 22 CTLV isolates as well as seven CTLV and five ASGV isolates previously reported from around the world revealed that the sequence identity ranged from 78.6% to 99.8%, indicating that there was a very low level of sequence heterogeneity among CTLV isolates of different geographic origins and hosts. Phylogenetic analyses of these 34 genomic sequences suggest that ASGV-MK (GenBank accession no.: MZ127820.1) has a closer relationship with CTLV than other four ASGV isolates. These results indicate that the evolutionary relationships of Chinese CTLV isolates may be associated with geographic origin and host species. |
| keywords:apple stem grooving virus citrus tatter leaf virus detection sequence alignment phyloge‐ netic analysis |
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