• 首页 | 期刊简介  | 
    编委会
    编委会
    青年编委会
     | 道德声明 | 投稿指南 | 联系我们 | 期刊订阅 | English
麦二叉蚜唾液蛋白基因Sg1655时空表达谱及其抑制小麦防御反应的功能
点此下载全文
引用本文:孙钰,张雨濛,刘晓蓓,陈巨莲,张勇,解海翠.麦二叉蚜唾液蛋白基因Sg1655时空表达谱及其抑制小麦防御反应的功能.植物保护学报,2024,51(1):20-27
DOI:10.13802/j.cnki.zwbhxb.2024.2023009
摘要点击次数:
全文下载次数:
作者单位E-mail
孙钰 河北科技师范学院, 河北省作物逆境生物学重点实验室, 秦皇岛 066600
中国农业科学院植物保护研究所, 植物病虫害综合治理全国重点实验室, 北京 100193 
 
张雨濛 中国农业科学院植物保护研究所, 植物病虫害综合治理全国重点实验室, 北京 100193  
刘晓蓓 中国农业科学院植物保护研究所, 植物病虫害综合治理全国重点实验室, 北京 100193  
陈巨莲 中国农业科学院植物保护研究所, 植物病虫害综合治理全国重点实验室, 北京 100193  
张勇 中国农业科学院植物保护研究所, 植物病虫害综合治理全国重点实验室, 北京 100193 zhangyong02@caas.cn 
解海翠 河北科技师范学院, 河北省作物逆境生物学重点实验室, 秦皇岛 066600 hcxie2008@126.comn 
中文摘要:为进一步明确麦二叉蚜Schizaphis graminum唾液蛋白Sg1655在调控小麦防御反应中的作用,基于麦二叉蚜唾液腺转录组数据,利用PCR技术克隆获取Sg1655开放阅读框,利用生物信息学软件对其序列进行分析;通过实时荧光定量PCR(real-time quantitative PCR,RT-qPCR)技术检测Sg1655在麦二叉蚜不同组织及不同龄期中的表达;利用细菌III型分泌系统将Sg1655导入到小麦叶片内,检测其瞬时表达对小麦胼胝质积累的影响。结果表明,Sg1655开放阅读框全长375 bp,编码125个氨基酸残基,N端1~21位为信号肽序列,预测蛋白分子量为14.90 kD。麦二叉蚜Sg1655氨基酸序列与玉米蚜Rhopalosiphum maidis同源序列相致性最高,为89.52%;麦二叉蚜Sg1655与玉米蚜同源序列聚为一个分支,亲缘关系最近。Sg1655在麦二叉蚜各组织中均有表达,其中在唾液腺中表达量最高,Sg1655在麦二叉蚜各个龄期均有表达,且在不同龄期之间无显著差异。小麦叶片内Sg1655瞬时表达可显著抑制小麦胼胝质积累,表明该蛋白可作为潜在效应子参与抑制小麦防御反应。
中文关键词:麦二叉蚜  唾液蛋白  Sg1655  基因克隆  序列比对  时空表达谱  功能分析
 
Spatio-temporal expression profiles and functional suppression of wheat defense responses by a salivary protein gene, Sg1655, from the spring-grain aphid Schizaphis graminum
Author NameAffiliationE-mail
Sun Yu Hebei Key Laboratory of Crop Stress Biology, Hebei Normal University of Science and Technology, Qinhuangdao 066600, Hebei Province, China
National Key Laboratory of Integrated Management of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China 
 
Zhang Yumeng National Key Laboratory of Integrated Management of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China  
Liu Xiaobei National Key Laboratory of Integrated Management of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China  
Chen Julian National Key Laboratory of Integrated Management of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China  
Zhang Yong National Key Laboratory of Integrated Management of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China zhangyong02@caas.cn 
Xie Haicui Hebei Key Laboratory of Crop Stress Biology, Hebei Normal University of Science and Technology, Qinhuangdao 066600, Hebei Province, China hcxie2008@126.comn 
Abstract:To further elucidate the role of the functionally unknown salivary protein Sg1655 in regulating the defense response of wheat, this study was carried out based on transcriptomic data from the salivary glands of Schizaphis graminum. The open reading frame of Sg1655 was obtained via PCR, and sequence analysis was performed using bioinformatics software. The expression level of Sg1655 in different aphid tissues and instars was measured using real-time quantitative PCR (RT-qPCR). Sg1655 was delivered into wheat leaves using a bacterial type III secretion system to examine the effect of its transient expression on callose accumulation in wheat. The results showed that the full-length open reading frame of Sg1655 was 375 bp, encoding 125 amino acid residues, with the N-terminal 1-21 sequence representing the signal peptide. The predicted protein molecular weight was 14.90 kD. Multiple sequence alignment revealed that the amino acid sequence of Sg1655 from S. graminum shared the highest sequence identity (89.52%) with the homologous sequence of Rhopalosiphum maidis. Sg1655 clustered into one branch with the homologous sequences of R. maidis, indicating a close genetic relationship between them. Sg1655 was found to be expressed in all aphid tissues, with the highest expression level in the salivary gland. In addition, Sg1655 expression was detected in all instars of S. graminum, with no significant difference in expression levels among various instars. Transient expression of Sg1655 significantly suppressed the accumulation of callose in wheat, indicating that the salivary protein Sg1655 may be involved in suppressing wheat defense responses as a potential effector.
keywords:Schizaphis graminum  salivary protein  Sg1655  gene cloning  sequence alignment  spatiotemporal expression profile  functional analysis
查看全文  查看/发表评论  下载PDF阅读器
您是本站第  8835570 版权所有:植物保护学报    京ICP备05006550号-2  
主管单位:中国科协 主办单位:中国植物保护学会、中国农业大学 地址:北京市圆明园西路2号 中国农业大学植物保护学院 植物保护学报编辑部
电话:010-62732528 电子邮件:zbxb@cau.edu.cn
技术支持:北京勤云科技发展有限公司