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宁夏回族自治区和浙江省光肩星天牛对柳属植物的寄主适应性
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引用本文:郭帅,谷奇,陆鹏飞,乔海莉,李玉春.宁夏回族自治区和浙江省光肩星天牛对柳属植物的寄主适应性.植物保护学报,2024,51(1):154-169
DOI:10.13802/j.cnki.zwbhxb.2024.2022105
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作者单位E-mail
郭帅 北京林业大学林学院, 林木资源高效生产全国重点实验室, 北京 100083  
谷奇 北京林业大学林学院, 林木资源高效生产全国重点实验室, 北京 100083  
陆鹏飞 北京林业大学林学院, 林木资源高效生产全国重点实验室, 北京 100083 lpengfei224@126.com 
乔海莉 中国医学科学院北京协和医学院药用植物研究所, 北京 100193 qhl193314@sina.com 
李玉春 内蒙古自治区阿拉善盟额济纳旗林业和草原局胡杨研究所, 额济纳 735400  
中文摘要:为解析光肩星天牛Anoplophora glabripennis在宁夏回族自治区和浙江省对金丝垂柳Salixbabylonica×S.alba-vitellina和旱柳S.matsudana的为害差异,通过代谢组学分析2种柳树木质部次生代谢产物的差异,利用16S rDNA测序技术检测来自2个地区的光肩星天牛幼虫在取食原生地寄主后肠道细菌群落的组成,测定其肠道消化酶、解毒酶和保护酶的活性,并将2种柳树的次生代谢产物分别与光肩星天牛肠道细菌及肠道酶进行Spearman相关性分析。结果表明,在金丝垂柳和旱柳木质部中共筛选出26种抗虫性差异代谢产物,其中苯甲酸及其衍生物、肉桂醛、吲哚及其衍生物和有机氮化合物在旱柳样本中含量更高;而黄酮类化合物、芪类化合物、咪唑并嘧啶和二嗪则在金丝垂柳样本中含量更高。以旱柳为食的幼虫肠道优势菌属为肠球菌属Enterococcus、拉乌尔菌属Raoultella、肠杆菌科未确定属、假单胞菌属Pseudomonas、肠杆菌属Enterobacter和纤维单胞菌属Cellulomonas;以金丝垂柳为食的幼虫肠道优势菌属则为拉乌尔菌属和Gibbsiella。取食旱柳的幼虫肠道中外切β-1,4-葡聚糖酶、羧酸酯酶、谷胱甘肽S转移酶和细胞色素P450酶的活性均显著高于取食金丝垂柳幼虫。金丝垂柳中黄酮类化合物含量高且变量投影重要性分析值较大,推测能被肠道内优势菌属拉乌尔菌属和Gibbsiella有效代谢,因此解毒酶活性处于较低水平,且拉乌尔菌属和Gibbsiella大量增殖;在取食旱柳的幼虫肠道中没有能有效代谢苯甲酸及其衍生物和肉桂醛等化学物质的细菌群落存在,从而引起解毒酶活性显著提升以减少受到的毒害作用。表明在光肩星天牛适应寄主植物的过程中,其肠道细菌在对寄主次生代谢产物的代谢过程中发挥着重要作用。
中文关键词:光肩星天牛  代谢组学  肠道微生物  肠道酶活  柳属
 
Host adaptation of Asian longhorn beetle Anoplophora glabripennis to Salix species in Ningxia and Zhejiang, China
Author NameAffiliationE-mail
Guo Shuai State Key Laboratory of Efficient Production of Forest Resources, College of Forestry, Beijing Forestry University, Beijing 100083, China  
Gu Qi State Key Laboratory of Efficient Production of Forest Resources, College of Forestry, Beijing Forestry University, Beijing 100083, China  
Lu Pengfei State Key Laboratory of Efficient Production of Forest Resources, College of Forestry, Beijing Forestry University, Beijing 100083, China lpengfei224@126.com 
Qiao Haili The Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China qhl193314@sina.com 
Li Yuchun Diversiform-leaved Poplar Institute, Ejina Banner Forestry and Grassland Bureau, Alxa League, Ejina 735400, Inner Mongolia Autonomous Region, China  
Abstract:To investigate the difference in the damages of Salix babylonica×S. alba-vitellina and S. matsudana caused by Asian longhorn beetle Anoplophora glabripennis in Ningxia Hui Autonomous Region and Zhejiang Province, the differences in the secondary metabolites of the xylem of the two willow trees were analyzed by metabolomics. 16S rDNA sequencing technology was used to detect the intestinal bacterial community composition of A. glabripennis larvae from two regions after feeding on S. babylonica×S. alba-vitellina or S. matsudana. The activities of intestinal digestive enzymes, detoxification enzymes and protective enzymes were also measured. Spearman correlation analysis was carried out between the secondary substances of two Salix species and intestinal bacteria and enzyme activity of A. glabripennis. The results showed that a total of 26 insect-resistant differential metabolites were screened from the xylem of S. babylonica×S. alba-vitellina and S. matsudana. Among them, the contents of benzoic acid and its derivatives, cinnamyl aldehyde, indole and its derivatives, and cinnamyl aldehyde were higher in S. matsudana. In contrast, the expressions of flavonoids, stilbene, ethyleneurea, and diazine were higher in S. babylonica×S. alba-vitellina. The dominant larval intestinal bacterial genera on S. matsudana were Enterococcus, Raoultella, unidentified Enterobacteriaceae, Pseudomonas, Enterobacter and Cellulomonas. Raoultella and Gibbsiella were the dominant larval intestinal bacteria of A. glabripennis feeding on S. babylonica×S. alba-vitellina. The activities of exo-β-1,4-glucanase, lipase, carboxylesterase, glutathione-S-transferase and cytochrome P450 activities in the intestinal samples of larvae feeding on S. matsudana were significantly higher than those on S. babylonica×S. alba-vitellina. The content of flavonoid in S. babylonica×S. albavitellina was high with a substantial variable importance in the projection value. It was speculated that the dominant bacterial species from the genus of Raoultella and Gibberella in the gut of A. glabripennis could easily metabolize flavonoids, leading to predominantly inactivated detoxification enzymes, which was accompanied by significant quantities of the bacterial community of Raoultella and Gibberella, although the levels of detoxification enzyme activity in the larval intestine were higher likely due to the absence of bacterial populations that can efficiently metabolize substances such as benzoic acid and its derivatives and cinnamyl aldehyde in the gut of A. glabripennis. The results indicated that, during the adaptation process of A. glabripennis to its host plant, the bacteria in its gut play a crucial role in the metabolism of the host’s secondary metabolites.
keywords:Anoplophora glabripennis  metabonomics  intestinal microbes  intestinal enzyme activity  Salix
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