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绿僵菌与FKBP52联合使用对西花蓟马的毒力
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引用本文:尹仙枫,朱凯辉,王芳,张蓉,王福莲,涂雄兵.绿僵菌与FKBP52联合使用对西花蓟马的毒力.植物保护学报,2024,51(1):178-185
DOI:10.13802/j.cnki.zwbhxb.2024.2023037
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作者单位E-mail
尹仙枫 长江大学农学院, 昆虫研究所, 农林病虫害预警与调控湖北省工程技术研究中心, 荆州 434025
中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193 
 
朱凯辉 中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193  
王芳 宁夏农林科学院植物保护研究所, 银川 750002  
张蓉 宁夏农林科学院植物保护研究所, 银川 750002  
王福莲 长江大学农学院, 昆虫研究所, 农林病虫害预警与调控湖北省工程技术研究中心, 荆州 434025 angfl_hb@163.com 
涂雄兵 中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193 txb1208@163.com 
中文摘要:为阐明绿僵菌与昆虫免疫抑制蛋白的增效机理,采用浸渍法测定昆虫免疫抑制蛋白FKBP52与金龟子绿僵菌Metarhizium anisopliae对西花蓟马Frankliniella occidentalis的毒力和其体内羧酸酯酶(carboxylesterase,CarE)、谷胱甘肽-S转移酶(glutathione-S transferase,GST)、酚氧化酶(phenoloxidase,PO)及几丁质酶活性的变化。结果显示,金龟子绿僵菌和FKBP52蛋白联合使用对西花蓟马的致死中时LT50为7.32 d,优于绿僵菌单用(10.87 d)或FKBP52蛋白单用的(14.15 d)。金龟子绿僵菌和FKBP52蛋白联合使用9 d时,西花蓟马的累计死亡率达81.11%,显著高于绿僵菌单用(45.56%)或FKBP52蛋白单用的(36.67%);金龟子绿僵菌和FKBP52蛋白联合使用的协同毒力指数为24.10,表现为增效作用。金龟子绿僵菌与FKBP52蛋白联合使用1 d后,西花蓟马体内CarE和PO活性小于金龟子绿僵菌单用后的活性;联合使用2 d后,西花蓟马体内几丁质酶活性小于绿僵菌单用后的活性,而CarE和GST活性均大于金龟子绿僵菌单用后的活性;联合使用3 d后,西花蓟马体内几丁质酶、CarE和PO活性均大于绿僵菌单用后的活性。表明与金龟子绿僵菌单用相比,金龟子绿僵菌和FKBP52蛋白联合使用后西花蓟马体内CarE、GST、PO和几丁质酶活性抑制或诱导效应与时间有关,这可能是两者具有增效作用的重要机制。
中文关键词:金龟子绿僵菌  西花蓟马  免疫抑制蛋白  保护酶  解毒酶
 
Synergistic toxicity of Metarhizium anisopliae and FKBP52 against the western flower thrips Frankliniella occidentalis
Author NameAffiliationE-mail
Yin Xianfeng Forewarning and Management of Agricultural and Forestry Pests, Hubei Engineering Technology Center, Institute of Entomological Science, College of Agriculture, Yangtze University, Jingzhou 434025, Hubei Province, China
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China 
 
Zhu Kaihui State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China  
Wang Fang Institute of Plant Protection, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750002, Ningxia Hui Autonomous Region, China  
Zhang Rong Institute of Plant Protection, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750002, Ningxia Hui Autonomous Region, China  
Wang Fulian Forewarning and Management of Agricultural and Forestry Pests, Hubei Engineering Technology Center, Institute of Entomological Science, College of Agriculture, Yangtze University, Jingzhou 434025, Hubei Province, China angfl_hb@163.com 
Tu Xiongbing State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China txb1208@163.com 
Abstract:To elucidate the synergistic mechanism of Metarhizium anisopliae and insect immunosuppressive proteins, the toxicity of the immunosuppressive protein FKBP52 and M. anisopliae to western flower thrips Frankliniella occidentalis were evaluated. The activities of carboxylesterase (CarE), glutathione-S transferase (GST), phenoloxidase (PO), and chitinase in F. intonsa also were assessed using the impregnation method. The results revealed that the median lethal time (LT50) for the combination of M. anisopliae and FKBP52 protein against F. intonsa was 7.32 d, demonstrating enhanced effectiveness compared to M. anisopliae (10.87 d) or FKBP52 protein (14.15 d) alone. When combined for nine days, the mortality rate of F. intonsa reached 81.11%, significantly higher than the rates observed with M. anisopliae (45.56%) or FKBP52 protein (36.67%) alone. The synergistic index of this mixture was 24.10, indicating a notable synergistic effect. Compared to the use of M. anisopliae alone, the activity levels of CarE and PO in F. intonsa initially decreased and then increased from day 1 to day 3 following the combined application of M. anisopliae and FKBP52 protein. The GST activity was significantly elevated only on day 2. Chitinase activity decreased on day 2 and then increased on day 3. These findings suggest that the time-dependent modulation of CarE, GST, PO, and chitinase activities in F. intonsa following the combined application of M. anisopliae and FKBP52 protein could be a crucial mechanism underpinning their synergistic effect.
keywords:Metarhizium anisopliae  Frankliniella occidentalis  insecticidal protein  protective enzyme  detoxification enzyme
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