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球孢白僵菌定殖提高番茄对灰霉病抗性及其作用机理
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引用本文:隋丽,路杨,周麟妍,李南楠,张正坤,李启云.球孢白僵菌定殖提高番茄对灰霉病抗性及其作用机理.植物保护学报,2024,51(1):203-210
DOI:10.13802/j.cnki.zwbhxb.2024.2023026
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作者单位E-mail
隋丽 吉林省农业科学院植物保护研究所, 吉林省农业微生物重点实验室, 农业农村部东北作物有害生物综合治理重点实验室, 公主岭 136100  
路杨 吉林省农业科学院植物保护研究所, 吉林省农业微生物重点实验室, 农业农村部东北作物有害生物综合治理重点实验室, 公主岭 136100  
周麟妍 吉林省农业科学院植物保护研究所, 吉林省农业微生物重点实验室, 农业农村部东北作物有害生物综合治理重点实验室, 公主岭 136100
吉林农业大学植物保护学院, 长春 130118 
 
李南楠 吉林省农业科学院植物保护研究所, 吉林省农业微生物重点实验室, 农业农村部东北作物有害生物综合治理重点实验室, 公主岭 136100
吉林农业大学植物保护学院, 长春 130118 
 
张正坤 吉林省农业科学院植物保护研究所, 吉林省农业微生物重点实验室, 农业农村部东北作物有害生物综合治理重点实验室, 公主岭 136100 zhangzhengkun1980@126.com 
李启云 吉林省农业科学院植物保护研究所, 吉林省农业微生物重点实验室, 农业农村部东北作物有害生物综合治理重点实验室, 公主岭 136100
吉林农业大学植物保护学院, 长春 130118
吉林农业科技学院, 吉林 132101 
qyli1225@126.com 
中文摘要:为明确球孢白僵菌Beauveria bassiana定殖对番茄植株抗病性的影响及作用机理,采用灌根法将球孢白僵菌芽生孢子悬浮液接种于番茄植株内,并通过人工接种灰霉菌Botrytis cinerea评价球孢白僵菌定殖后番茄对灰霉病的抗性水平;检测灰霉菌胁迫下番茄植株不同位置叶片内球孢白僵菌的相对含量;测定番茄叶片内草酸氧化酶(oxalate oxidase,OXO)、几丁质酶(chitinase,CHI)和ATP合成酶(ATP synthase,atpA)3种抗病相关基因的表达量。结果表明,球孢白僵菌定殖能够提高番茄植株对灰霉病的抗性,接种灰霉菌第5天,番茄植株发病率、病斑直径和病情指数分别下降了61.6%、41.4%和26.4%;在灰霉菌胁迫下番茄植株内球孢白僵菌偏好于在病原菌感染位置定向聚集,并且引起植物抗病基因OXOCHIatpA的表达量上调。表明球孢白僵菌能通过内生定殖与植物互作提高植物抗病性,在植物病害生物防治领域有较大应用潜力。
中文关键词:球孢白僵菌  内生定殖  番茄灰霉病  抗性  作用机理  招募作用  抗病基因
 
Enhanced tomato resistance to gray mold by fungus Beauveria bassiana colonization and its mechanism of action
Author NameAffiliationE-mail
Sui Li Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture and Rural Areas
Jilin Key Laboratory of Agricultural Microbiology, Institute of Plant Protection, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, Jilin Province, China 
 
Lu Yang Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture and Rural Areas
Jilin Key Laboratory of Agricultural Microbiology, Institute of Plant Protection, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, Jilin Province, China 
 
Zhou Linyan Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture and Rural Areas
Jilin Key Laboratory of Agricultural Microbiology, Institute of Plant Protection, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, Jilin Province, China
College of Plant Protection, Jilin Agricultural University, Changchun 130118, Jilin Province, China 
 
Li Nannan Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture and Rural Areas
Jilin Key Laboratory of Agricultural Microbiology, Institute of Plant Protection, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, Jilin Province, China
College of Plant Protection, Jilin Agricultural University, Changchun 130118, Jilin Province, China 
 
Zhang Zhengkun Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture and Rural Areas
Jilin Key Laboratory of Agricultural Microbiology, Institute of Plant Protection, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, Jilin Province, China 
zhangzhengkun1980@126.com 
Li Qiyun Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture and Rural Areas
Jilin Key Laboratory of Agricultural Microbiology, Institute of Plant Protection, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, Jilin Province, China
College of Plant Protection, Jilin Agricultural University, Changchun 130118, Jilin Province, China
Jilin Agricultural Science and Technology University, Jilin 132101, Jilin Province, China 
qyli1225@126.com 
Abstract:In order to clarify the effect of fungus Beauveria bassiana colonization on tomato plant disease resistance and its mechanism of action, conidial blastospores suspension of B. bassiana were inoculated into tomato plants with root irrigation, and the resistance of tomato against gray mold caused by Botrytis cinerea after colonization by B. bassiana was evaluated with artificial inoculation; the relative content of B. bassiana at different positions of tomato leaves under the stress of B. cinerea was detected. The expression levels of three disease resistance genes, including oxalate oxidase (OXO), chitinase (CHI) and ATP synthase (atpA) in tomato leaves were determined. The results showed that B. bassiana colonization could improve resistance of tomato plants to gray mold, on the 5th day after B. cinerea inoculation, the incidence rate, lesion diameter and disease index in tomato decreased by 61.6%, 41.4% and 26.4%, respectively. It is noteworthy that B. bassiana was more likely to accumulate in the infected leaves and especially areas close to the infection sites, and up-regulated the expression levels of OXO, CHI and atpA genes. The results indicated that B. bassiana could improve plant disease resistance through endophytic colonization and interaction with host plants, and have great application potential in the field for the biological control of plant diseases.
keywords:Beauveria bassiana  endophytic  tomato gray mold  resistance  mechanism  recruitment function  disease resistance gene
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