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糖苷水解酶基因FvGH16-2参与拟轮枝镰孢菌生长发育及致病性
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引用本文:任志国,刘宁,陈悦,孙蔓莉,曹志艳,董金皋.糖苷水解酶基因FvGH16-2参与拟轮枝镰孢菌生长发育及致病性.植物保护学报,2024,51(2):342-351
DOI:10.13802/j.cnki.zwbhxb.2024.2023080
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作者单位E-mail
任志国 河北农业大学植物保护学院, 保定 071000
河北农业大学, 河北省植物生理与分子病理学重点实验室, 华北作物改良与调控国家重点实验室, 保定 071000 
 
刘宁 河北农业大学植物保护学院, 保定 071000
河北农业大学, 河北省植物生理与分子病理学重点实验室, 华北作物改良与调控国家重点实验室, 保定 071000 
 
陈悦 河北农业大学, 河北省植物生理与分子病理学重点实验室, 华北作物改良与调控国家重点实验室, 保定 071000
河北农业大学生命科学学院, 保定 071000 
 
孙蔓莉 河北农业大学植物保护学院, 保定 071000
河北农业大学, 河北省植物生理与分子病理学重点实验室, 华北作物改良与调控国家重点实验室, 保定 071000 
 
曹志艳 河北农业大学植物保护学院, 保定 071000
河北农业大学, 河北省植物生理与分子病理学重点实验室, 华北作物改良与调控国家重点实验室, 保定 071000 
caozhiyan@hebau.edu.cn 
董金皋 河北农业大学植物保护学院, 保定 071000
河北农业大学, 河北省植物生理与分子病理学重点实验室, 华北作物改良与调控国家重点实验室, 保定 071000 
shmdjg@hebau.edu.cn 
中文摘要:为明确糖苷水解酶16(glycoside hydrolase,GH16)家族基因在拟轮枝镰孢菌Fusarium verticillioides生长发育和致病过程中的作用,利用生物信息学分析软件对其GH16家族成员进行鉴定,采用转录组分析及实时荧光定量PCR技术对GH16家族成员在不同培养温度下的表达水平进行检测,并通过遗传转化法对家族成员FvGH16-2基因进行敲除及回补,分析其在拟轮枝镰孢菌生长发育及致病过程中的作用。结果显示,在拟轮枝镰孢菌基因组中共鉴定到23个GH16家族基因,其中FvCH16-2FvCH16-4FvCH16-12等多个上调表达基因与病菌的生长和抗逆性相关。敲除FvGH16-2基因导致拟轮枝镰孢菌的生长速率及产孢能力降低,细胞壁完整性受损,对H2O2更加敏感,同时对玉米籽粒和茎秆的致病力减弱。表明FvGH16-2基因在拟轮枝镰孢菌生长发育和致病过程中发挥着重要作用,是拟轮枝镰孢菌细胞壁完整性和致病性所必需的。
中文关键词:糖苷水解酶家族  拟轮枝镰孢菌  玉米病害  基因表达  功能分析
 
Glycoside hydrolase gene FvGH16-2 is involved in the growth and pathogenicity of mycotoxigenic fungus Fusarium verticillioides
Author NameAffiliationE-mail
Ren Zhiguo College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China
Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, State Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding 071000, Hebei Province, China 
 
Liu Ning College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China
Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, State Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding 071000, Hebei Province, China 
 
Chen Yue Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, State Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding 071000, Hebei Province, China
College of Life Science, Hebei Agricultural University, Baoding 071000, Hebei Province, China 
 
Sun Manli College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China
Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, State Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding 071000, Hebei Province, China 
 
Cao Zhiyan College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China
Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, State Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding 071000, Hebei Province, China 
caozhiyan@hebau.edu.cn 
Dong Jingao College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China
Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, State Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding 071000, Hebei Province, China 
shmdjg@hebau.edu.cn 
Abstract:To clarify the role of glycoside hydrolase 16 (GH16) family genes in the growth, development, and pathogenesis of mycotoxigenic fungus Fusarium verticillioides, bioinformatics software was used to identify GH16 family members, and transcriptome and real-time fluorescence quantitative PCR techniques were used to analyze the expression levels of GH16 family members at different culture temperatures. The FvGH16-2 gene was knocked out and replaced by genetic transformation to analyze its role in growth, development, and pathogenesis of F. verticillioides. The results showed that a total of 23 GH16 family genes were identified in F. verticillioides genome, among which FvCH16-2, FvCH16-4, FvCH16-12 and other up-regulated genes were associated with the growth and stress resistance of pathogens. FvGH16-2 gene knockout resulted in decreased growth rate and conidial production of F. verticillioides, impaired cell wall integrity, and increased sensitivity to H2O2. In addition, FvGH16-2 gene knockout reduced the pathogenicity of F. verticillioides on maize seeds and maize stalks. These results indicated that the FvGH16-2 gene played an important role in the growth, development, and pathogenesis of F. verticillioides, and was essential for the integrity of the cell wall and pathogenicity of F. verticillioides.
keywords:glycoside hydrolase  Fusarium verticillioides  maize disease  gene expression  functional analysis
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