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水稻锯齿叶矮缩病毒对褐飞虱生长发育和生殖的影响

引用本文：刘健丽,李学文,欧阳钰莹,郭齐汤,尤民生,林胜.水稻锯齿叶矮缩病毒对褐飞虱生长发育和生殖的影响.植物保护学报,2024,51(2):377-382

DOI：10.13802/j.cnki.zwbhxb.2024.2023031

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作者	单位	E-mail
刘健丽	福建农林大学植物保护学院, 闽台作物有害生物生态防控国家重点实验室, 海峡两岸特色作物安全生产省部共建协同创新中心, 福建省农产品安全研究平台, 害虫绿色防控福建省高校重点实验室, 福州 350002
李学文	福建农林大学植物保护学院, 闽台作物有害生物生态防控国家重点实验室, 海峡两岸特色作物安全生产省部共建协同创新中心, 福建省农产品安全研究平台, 害虫绿色防控福建省高校重点实验室, 福州 350002
欧阳钰莹	福建农林大学植物保护学院, 闽台作物有害生物生态防控国家重点实验室, 海峡两岸特色作物安全生产省部共建协同创新中心, 福建省农产品安全研究平台, 害虫绿色防控福建省高校重点实验室, 福州 350002
郭齐汤	三明市烟草公司大田分公司, 福建三明 366100
尤民生	福建农林大学植物保护学院, 闽台作物有害生物生态防控国家重点实验室, 海峡两岸特色作物安全生产省部共建协同创新中心, 福建省农产品安全研究平台, 害虫绿色防控福建省高校重点实验室, 福州 350002	msyou@fafu.edu.cn
林胜	福建农林大学植物保护学院, 闽台作物有害生物生态防控国家重点实验室, 海峡两岸特色作物安全生产省部共建协同创新中心, 福建省农产品安全研究平台, 害虫绿色防控福建省高校重点实验室, 福州 350002	linsn001@163.com

中文摘要:为明确水稻锯齿叶矮缩病毒（rice ragged stunt virus，RRSV）对褐飞虱Nilaparvata lugens生长发育和生殖的影响，分别比较带毒褐飞虱与未带毒褐飞虱的若虫发育历期、成虫寿命、性比、翅型及产卵量，并利用实时荧光定量PCR（real-time quantitative PCR，RT-qPCR）技术分析RRSV侵染后褐飞虱体内卵黄原蛋白（vitellogenin，Vg）与其受体（vitellogenin receptor，VgR）以及海藻糖代谢途径中相关基因表达量的情况。结果显示，带毒褐飞虱的若虫历期显著高于未带毒褐飞虱的若虫历期，分别为17.56 d和15.90 d；带毒褐飞虱的成虫寿命、雌虫比例和短翅比例均高于未带毒褐飞虱的，但两者间均无显著差异；带毒褐飞虱较未带毒褐飞虱单雌日产卵量显著提升，分别为11.26粒和6.45粒；带毒褐飞虱的Vg、VgR和海藻糖转运蛋白基因（trehalose transporter，TRET）表达量均显著高于未带毒褐飞虱的。表明RRSV侵染会显著延长褐飞虱若虫发育历期，同时可能通过上调Vg、VgR以及TRET的表达量以促进褐飞虱生殖。

中文关键词:水稻锯齿叶矮缩病毒褐飞虱生长发育繁殖力

Effects of rice ragged stunt virus on the growth, development and reproduction of the brown planthopper Nilaparvata lugens

Author Name	Affiliation	E-mail
Liu Jianli	Key Laboratory of Green Pest Control, Fujian Province University Fujian Agricultural Product Safety Research Platform Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops State Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, China
Li Xuewen	Key Laboratory of Green Pest Control, Fujian Province University Fujian Agricultural Product Safety Research Platform Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops State Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, China
Ouyang Yuying	Key Laboratory of Green Pest Control, Fujian Province University Fujian Agricultural Product Safety Research Platform Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops State Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, China
Guo Qitang	Datian Branch of Sanming Tobacco Company, Sanming 366100, Fujian Province, China
You Minsheng	Key Laboratory of Green Pest Control, Fujian Province University Fujian Agricultural Product Safety Research Platform Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops State Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, China	msyou@fafu.edu.cn
Lin Sheng	Key Laboratory of Green Pest Control, Fujian Province University Fujian Agricultural Product Safety Research Platform Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops State Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, China	linsn001@163.com

Abstract:To clarify the effects of rice ragged stunt virus (RRSV) on the growth, development, and reproduction of the brown planthopper Nilaparvata lugens, we conducted laboratory breeding experiments to assess various growth and life table parameters, including larval duration, adult longevity, egg production, sex ratio, and brachypterous ratio of N. lugens. Additionally, we measured the expression levels of vitellogenin (Vg), vitellogenin receptor (VgR), and genes involved in the trehalose metabolic process using real-time quantitative PCR (RT-qPCR) between viruliferous and non-viruliferous N. lugens. The results showed that the nymphal duration of viruliferous N. lugens was significantly prolonged compared to non-viruliferous N. lugens, with durations of 17.56 d and 15.90 d, respectively. Moreover, viruliferous N. lugens displayed slightly higher adult longevity, female ratio, and brachypterous ratio compared to their non-viruliferous counterparts. The fecundity of viruliferous N. lugens was significantly elevated, with an average of 11.26 eggs per female compared to 6.45 eggs per female for non-viruliferous individuals. Furthermore, significantly higher expression levels of Vg, VgR, and the trehalose transporter gene (TRET) were observed in viruliferous N. lugens compared to non-viruliferous individuals. These results indicated that RRSV invasion could significantly prolong the nymphal duration and increase N. lugens fecundity, potentially attributed to the up-regulated expression levels of Vg, VgR, and TRET.

keywords:rice ragged stunt virus Nilaparvata lugens growth and development fecundity

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