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亚洲小车蝗响应高效氯氰菊酯胁迫的转录组及抗性相关基因分析

引用本文：高海燕,杨旭兵,闫锋,孙彦楠,张玉,高书晶.亚洲小车蝗响应高效氯氰菊酯胁迫的转录组及抗性相关基因分析.植物保护学报,2024,51(2):421-431

DOI：10.13802/j.cnki.zwbhxb.2024.2023044

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作者	单位	E-mail
高海燕	中国农业科学院草原研究所, 农业农村部人工草地生物灾害监测与绿色防控重点实验室, 呼和浩特 010010
杨旭兵	中国农业科学院草原研究所, 农业农村部人工草地生物灾害监测与绿色防控重点实验室, 呼和浩特 010010
闫锋	鄂尔多斯生态环境职业学院, 内蒙古鄂尔多斯 017010
孙彦楠	鄂尔多斯市林业和草原事业发展中心, 内蒙古鄂尔多斯 017010
张玉	中国农业科学院草原研究所, 农业农村部人工草地生物灾害监测与绿色防控重点实验室, 呼和浩特 010010	zhangyu11@caas.cn
高书晶	中国农业科学院草原研究所, 农业农村部人工草地生物灾害监测与绿色防控重点实验室, 呼和浩特 010010	shujinggao688@163.com

中文摘要:为筛选亚洲小车蝗Oedaleus asiaticus响应高效氯氰菊酯胁迫的抗性相关基因和代谢通路，解析亚洲小车蝗对高效氯氰菊酯的抗性机制，利用Illumina Hiseq 4000高通量测序平台对高效氯氰菊酯（LD₁₀=0.008 μg/头）处理48 h前后的亚洲小车蝗3龄蝗蝻进行转录组测序，采用BLAST、edgeR以及Trinity等生物信息学软件进行功能注释、差异表达基因分析及代谢通路分析。结果显示，从亚洲小车蝗中共筛选到743个响应高效氯氰菊酯胁迫的差异表达基因，包括208个上调基因和535个下调基因，其中有168个差异表达基因显著富集到GO数据库的细胞组分、分子功能和生物学过程三大功能分类中，162个差异表达基因显著富集到20条不同的代谢通路中，主要涉及核糖体、碳代谢、糖酵解/糖异生和氨基酸生物合成等代谢通路，其中多个代谢通路与昆虫抗药性相关。进一步分析发现与昆虫抗药性相关的差异表达基因有47个，包括编码谷胱甘肽S-转移酶、乙醇脱氢酶、细胞色素P450基因家族、热激蛋白、羧酸酯酶、黏蛋白、多功能氧化酶以及羰基还原酶等蛋白的基因，推测这些基因在亚洲小车蝗响应高效氯氰菊酯胁迫的过程中发挥了一定作用。

中文关键词:亚洲小车蝗转录组高效氯氰菊酯抗药性差异表达

Analysis of transcriptome and resistance-related genes induced by beta-cypermethrin in the grasshopper Oedaleus asiaticus (Orthoptera: Oedipodidae)

Author Name	Affiliation	E-mail
Gao Haiyan	Key Laboratory of Biohazard Monitoring, Green Prevention and Control for Artificial Grassland, Ministry of Agriculture and Rural Affairs Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot 010010, Inner Mongolia Autonomous Region, China
Yang Xubing	Key Laboratory of Biohazard Monitoring, Green Prevention and Control for Artificial Grassland, Ministry of Agriculture and Rural Affairs Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot 010010, Inner Mongolia Autonomous Region, China
Yan Feng	Ordos Vocational College of Eco-environment, Ordos 017010, Inner Mongolia Autonomous Region, China
Sun Yannan	Ordos Forestry and Grassland Enterprise Development Center, Ordos 017010, Inner Mongolia Autonomous Region, China
Zhang Yu	Key Laboratory of Biohazard Monitoring, Green Prevention and Control for Artificial Grassland, Ministry of Agriculture and Rural Affairs Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot 010010, Inner Mongolia Autonomous Region, China	zhangyu11@caas.cn
Gao Shujing	Key Laboratory of Biohazard Monitoring, Green Prevention and Control for Artificial Grassland, Ministry of Agriculture and Rural Affairs Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot 010010, Inner Mongolia Autonomous Region, China	shujinggao688@163.com

Abstract:This study aimed to identify resistance-related genes and metabolic pathways in Oedaleus asiaticus in response to beta-cypermethrin stress and elucidate the resistance mechanism of O. asiaticus to beta-cypermethrin. The 3rd instar nymphs of O. asiaticus were treated with beta-cypermethrin (LD₁₀= 0.008 μg/nymph) for 48 h. The transcriptome sequencing was performed on preand post-processing samples by using the Illummina Hiseq 4000 high-throughput sequencing platform. Functional annotation, differential expression gene (DEG) analysis, and metabolic pathway analysis were performed using bioinformatics tools BLAST, edgeR, and Trinity. The results revealed 743 DEGs in O. asiaticus after treated with beta-cypermethrin, including 208 up-regulated genes and 535 down-regulated genes. Among them, 168 DEGs were significantly enriched in three functional categories (cell components, molecular functions and biological processes) in the GO database, while 162 DEGs were significantly enriched in 20 different metabolic pathways, which were mainly involved in the metabolic pathways such as ribosome, carbon metabolism, glycolysis, and amino acid biosynthesis, with several metabolic pathways related to insecticide resistance. Further analysis identified 47 DEGs related to insect resistance, including glutathione S-transferase, alcohol dehydrogenase, cytochrome P450 gene family, heat shock protein, carboxylesterase, mucin, multifunctional oxidase, and carbonyl reductase. These genes play a role in the resistance of O. asiaticus to beta-cypermethrin stress.

keywords:Oedaleus asiaticus transcriptome beta-cypermethrin insecticide resistance differential expression

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