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凹唇壁蜂雌成虫滞育期、休眠期和破茧期肠道菌群多样性分析
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引用本文:王光召,马艺翔,秦敬泽,游银伟,马朋涛,李丽莉,门兴元,宋莹莹,张凯,李霞,王桂萍,于毅.凹唇壁蜂雌成虫滞育期、休眠期和破茧期肠道菌群多样性分析.植物保护学报,2024,51(3):556-567
DOI:10.13802/j.cnki.zwbhxb.2024.2023057
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作者单位E-mail
王光召 烟台大学生命科学学院, 山东 烟台 264005  
马艺翔 烟台大学生命科学学院, 山东 烟台 264005
山东省农业科学院植物保护研究所, 济南 250100 
 
秦敬泽 山东农业工程学院林业工程学院, 德州 251100  
游银伟 山东省农业科学院植物保护研究所, 济南 250100  
马朋涛 烟台大学生命科学学院, 山东 烟台 264005  
李丽莉 山东省农业科学院植物保护研究所, 济南 250100  
门兴元 山东省农业科学院植物保护研究所, 济南 250100  
宋莹莹 山东省农业科学院植物保护研究所, 济南 250100  
张凯 山东农业工程学院林业工程学院, 德州 251100  
李霞 山东省农业科学院植物保护研究所, 济南 250100  
王桂萍 山东省农业科学院植物保护研究所, 济南 250100 wangguiping1018@126.com 
于毅 山东省农业科学院植物保护研究所, 济南 250100 robertyuyi@163.com 
中文摘要:为明确凹唇壁蜂Osmia excavata雌成虫滞育期至破茧期肠道菌群的多样性及差异,解析其在宿主健康与调控宿主生长发育中的作用,采用Illumina NovaSeq二代高通量测序技术对不同时期肠道细菌16S rRNA V3~V4区域进行测序,分析雌成虫滞育期至破茧期肠道菌群的多样性变化。结果显示,凹唇壁蜂雌成虫在滞育期至破茧期肠道细菌菌群中共检测到41门96纲198目325科637属,其中核心菌门是变形菌门,相对丰度介于75.98%~91.41%之间;核心菌属是Sodalis,相对丰度介于59.65%~74.66%之间。Alpha多样性分析显示,从滞育期到休眠期,雌成虫肠道内菌群多样性指数明显升高,从休眠期至破茧期,其肠道菌群多样性指数出现降低趋势;休眠期其肠道菌群的物种丰富度和均匀度均最高,滞育期其肠道菌群的物种丰富度和均匀度均最低;Beta多样性分析结果显示,从滞育期至破茧期,其肠道菌群结构相对稳定;LEfSe分析结果显示,从滞育期至破茧期其肠道菌群在门、纲、目、科、属水平均存在显著差异,且差异显著菌群主要出现在休眠期。预测的不同时期的肠道菌群功能不同,这与其成虫解除滞育进入休眠再破茧的生理过程密切相关。表明凹唇壁蜂雌成虫从滞育期至破茧期肠道核心细菌种类一致,群落结构相对稳定,但是群落多样性发生明显变化。
中文关键词:凹唇壁蜂  滞育  休眠  破茧  肠道菌群  多样性
 
Analysis of intestinal microbiota diversity in female adult diapause, post-diapause and cocoon-breaking stages of mason bee Osmia excavata
Author NameAffiliationE-mail
Wang Guangzhao School of Life Sciences, Yantai University, Yantai 264005, Shandong Province, China  
Ma Yixiang School of Life Sciences, Yantai University, Yantai 264005, Shandong Province, China
Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong Province, China 
 
Qin Jingze School of Forestry Engineering, Shandong Agriculture and Engineering University, Dezhou 251100, Shandong Province, China  
You Yinwei Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong Province, China  
Ma Pengtao School of Life Sciences, Yantai University, Yantai 264005, Shandong Province, China  
Li Lili Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong Province, China  
Men Xingyuan Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong Province, China  
Song Yingying Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong Province, China  
Zhang Kai School of Forestry Engineering, Shandong Agriculture and Engineering University, Dezhou 251100, Shandong Province, China  
Li Xia Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong Province, China  
Wang Guiping Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong Province, China wangguiping1018@126.com 
Yu Yi Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong Province, China robertyuyi@163.com 
Abstract:To clarify the diversity and differences in the intestinal flora of mason bee Osmia excavata adult females from the diapause phase to the cocoon-breaking stage, and to analyze their role in host health and regulation of host growth and development, the Illumina NovaSeq second-generation highthroughput sequencing method was used to sequence the 16S rRNA V3 to V4 regions of gut bacteria and analyze changes in gut flora diversity from diapause to cocoon-breaking stages. The results revealed a total of 41 phyla, 96 classes, 198 orders, 325 families, and 637 genera in the intestinal bacterial flora of O. excavata female adults during these stages. The core phylum in the intestinal bacterial flora of O. excavata adult females during the diapause phase to cocoon-breaking stage was Proteobacteria, with relative abundance ranging from 75.98% to 91.41%; the core genus was Sodalis, with relative abundance ranging from 59.65% to 74.66%. Alpha diversity analyses showed that the diversity index of the intestinal flora increased significantly from the diapause phase to the post-diapause phase, followed by a decreasing trend from the diapause phase to the post-diapause phase. The species richness and homogeneity of gut flora were highest during the post-diapause period and lowest during the diapause period. Beta diversity analysis showed that the structure of the gut flora remained relatively stable from the diapause phase to the cocoon-breaking phase. LEfSe analysis revealed significant differences in intestinal flora at the level of phylum, class, order, family, and genus from the diapause phase to the cocoonbreaking phase, with the most significant differences appearing during the post-diapause period. The predicted functions of the gut flora at different times are closely related to the physiological process from diapause to post-diapause and cocoon-breaking in adults. This study indicated that O. excavata female adults maintained the same species of core gut bacteria from the diapause phase to the cocoonbreaking phase. While the community structure was relatively stable, the diversity of the community changed significantly.
keywords:Osmia excavata  diapause  post-diapause  cocoon-breaking  intestinal microbiota  diversity
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