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凹唇壁蜂雌成虫滞育期、休眠期和破茧期肠道菌群多样性分析

引用本文：王光召,马艺翔,秦敬泽,游银伟,马朋涛,李丽莉,门兴元,宋莹莹,张凯,李霞,王桂萍,于毅.凹唇壁蜂雌成虫滞育期、休眠期和破茧期肠道菌群多样性分析.植物保护学报,2024,51(3):556-567

DOI：10.13802/j.cnki.zwbhxb.2024.2023057

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作者	单位	E-mail
王光召	烟台大学生命科学学院, 山东烟台 264005
马艺翔	烟台大学生命科学学院, 山东烟台 264005 山东省农业科学院植物保护研究所, 济南 250100
秦敬泽	山东农业工程学院林业工程学院, 德州 251100
游银伟	山东省农业科学院植物保护研究所, 济南 250100
马朋涛	烟台大学生命科学学院, 山东烟台 264005
李丽莉	山东省农业科学院植物保护研究所, 济南 250100
门兴元	山东省农业科学院植物保护研究所, 济南 250100
宋莹莹	山东省农业科学院植物保护研究所, 济南 250100
张凯	山东农业工程学院林业工程学院, 德州 251100
李霞	山东省农业科学院植物保护研究所, 济南 250100
王桂萍	山东省农业科学院植物保护研究所, 济南 250100	wangguiping1018@126.com
于毅	山东省农业科学院植物保护研究所, 济南 250100	robertyuyi@163.com

中文摘要:为明确凹唇壁蜂Osmia excavata雌成虫滞育期至破茧期肠道菌群的多样性及差异，解析其在宿主健康与调控宿主生长发育中的作用，采用Illumina NovaSeq二代高通量测序技术对不同时期肠道细菌16S rRNA V3~V4区域进行测序，分析雌成虫滞育期至破茧期肠道菌群的多样性变化。结果显示，凹唇壁蜂雌成虫在滞育期至破茧期肠道细菌菌群中共检测到41门96纲198目325科637属，其中核心菌门是变形菌门，相对丰度介于75.98%~91.41%之间；核心菌属是Sodalis，相对丰度介于59.65%~74.66%之间。Alpha多样性分析显示，从滞育期到休眠期，雌成虫肠道内菌群多样性指数明显升高，从休眠期至破茧期，其肠道菌群多样性指数出现降低趋势；休眠期其肠道菌群的物种丰富度和均匀度均最高，滞育期其肠道菌群的物种丰富度和均匀度均最低；Beta多样性分析结果显示，从滞育期至破茧期，其肠道菌群结构相对稳定；LEfSe分析结果显示，从滞育期至破茧期其肠道菌群在门、纲、目、科、属水平均存在显著差异，且差异显著菌群主要出现在休眠期。预测的不同时期的肠道菌群功能不同，这与其成虫解除滞育进入休眠再破茧的生理过程密切相关。表明凹唇壁蜂雌成虫从滞育期至破茧期肠道核心细菌种类一致，群落结构相对稳定，但是群落多样性发生明显变化。

中文关键词:凹唇壁蜂滞育休眠破茧肠道菌群多样性

Analysis of intestinal microbiota diversity in female adult diapause, post-diapause and cocoon-breaking stages of mason bee Osmia excavata

Author Name	Affiliation	E-mail
Wang Guangzhao	School of Life Sciences, Yantai University, Yantai 264005, Shandong Province, China
Ma Yixiang	School of Life Sciences, Yantai University, Yantai 264005, Shandong Province, China Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong Province, China
Qin Jingze	School of Forestry Engineering, Shandong Agriculture and Engineering University, Dezhou 251100, Shandong Province, China
You Yinwei	Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong Province, China
Ma Pengtao	School of Life Sciences, Yantai University, Yantai 264005, Shandong Province, China
Li Lili	Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong Province, China
Men Xingyuan	Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong Province, China
Song Yingying	Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong Province, China
Zhang Kai	School of Forestry Engineering, Shandong Agriculture and Engineering University, Dezhou 251100, Shandong Province, China
Li Xia	Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong Province, China
Wang Guiping	Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong Province, China	wangguiping1018@126.com
Yu Yi	Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong Province, China	robertyuyi@163.com

Abstract:To clarify the diversity and differences in the intestinal flora of mason bee Osmia excavata adult females from the diapause phase to the cocoon-breaking stage, and to analyze their role in host health and regulation of host growth and development, the Illumina NovaSeq second-generation highthroughput sequencing method was used to sequence the 16S rRNA V3 to V4 regions of gut bacteria and analyze changes in gut flora diversity from diapause to cocoon-breaking stages. The results revealed a total of 41 phyla, 96 classes, 198 orders, 325 families, and 637 genera in the intestinal bacterial flora of O. excavata female adults during these stages. The core phylum in the intestinal bacterial flora of O. excavata adult females during the diapause phase to cocoon-breaking stage was Proteobacteria, with relative abundance ranging from 75.98% to 91.41%; the core genus was Sodalis, with relative abundance ranging from 59.65% to 74.66%. Alpha diversity analyses showed that the diversity index of the intestinal flora increased significantly from the diapause phase to the post-diapause phase, followed by a decreasing trend from the diapause phase to the post-diapause phase. The species richness and homogeneity of gut flora were highest during the post-diapause period and lowest during the diapause period. Beta diversity analysis showed that the structure of the gut flora remained relatively stable from the diapause phase to the cocoon-breaking phase. LEfSe analysis revealed significant differences in intestinal flora at the level of phylum, class, order, family, and genus from the diapause phase to the cocoonbreaking phase, with the most significant differences appearing during the post-diapause period. The predicted functions of the gut flora at different times are closely related to the physiological process from diapause to post-diapause and cocoon-breaking in adults. This study indicated that O. excavata female adults maintained the same species of core gut bacteria from the diapause phase to the cocoonbreaking phase. While the community structure was relatively stable, the diversity of the community changed significantly.

keywords:Osmia excavata diapause post-diapause cocoon-breaking intestinal microbiota diversity

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