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草地贪夜蛾核型多角体病毒对草地贪夜蛾的毒力及其侵染特征
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引用本文:彭倩,易磊,曾洋,施玉双,傅悦,谌祖文.草地贪夜蛾核型多角体病毒对草地贪夜蛾的毒力及其侵染特征.植物保护学报,2024,51(3):629-635
DOI:10.13802/j.cnki.zwbhxb.2024.2023108
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作者单位E-mail
彭倩 黄冈师范学院生物与农业资源学院, 经济林木种质改良与资源综合利用湖北省重点实验室, 湖北 黄冈 438000  
易磊 黄冈市疾病预防控制中心, 湖北 黄冈 438000  
曾洋 黄冈师范学院生物与农业资源学院, 经济林木种质改良与资源综合利用湖北省重点实验室, 湖北 黄冈 438000  
施玉双 黄冈师范学院生物与农业资源学院, 经济林木种质改良与资源综合利用湖北省重点实验室, 湖北 黄冈 438000  
傅悦 黄冈师范学院生物与农业资源学院, 经济林木种质改良与资源综合利用湖北省重点实验室, 湖北 黄冈 438000  
谌祖文 黄冈师范学院生物与农业资源学院, 经济林木种质改良与资源综合利用湖北省重点实验室, 湖北 黄冈 438000 zuwenchen@hgnu.edu.cn 
中文摘要:为明确草地贪夜蛾核型多角体病毒(Spodoptera frugiperda multiple nucleopolyhedrovirus,SfMNPV)对草地贪夜蛾Spodoptera frugiperda 的毒力及其体内外侵染特性,于室内测定SfMNPV对草地贪夜蛾的毒力,采用苏木精-伊红(hematoxylin-eosin,HE)染色分析SfMNPV侵染后草地贪夜蛾的组织病理学特征,通过实时荧光定量PCR(real-time fluorescence quantitative PCR,qPCR)技术分析SfMNPV在草地贪夜蛾体内和Sf9细胞系中的复制特征。结果表明,SfMNPV对草地贪夜蛾2龄幼虫的LC50和LT50分别为7.39×105 OBs/mL和3.82 d;SfMNPV可侵染草地贪夜蛾幼虫的真皮、气管、脂肪体和中肠等组织,未侵染肌肉组织;SfMNPV侵染96 h后草地贪夜蛾体内SfMNPV基因组拷贝数最大,为3.91×106 拷贝数/ng,SfMNPV侵染96 h后Sf9细胞培养上清液中SfMNPV基因组拷贝数最大,为2.08×107 拷贝数/mL。
中文关键词:草地贪夜蛾  杆状病毒  杀虫毒力  侵染特性  组织
 
Virulence and infectious characteristics of a Spodoptera frugiperda multiple nucleopolyhedrovirus to fall armyworm Spodoptera frugiperda
Author NameAffiliationE-mail
Peng Qian Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, College of Biology and Agricultural Resources, Huanggang Normal University, Huanggang 438000, Hubei Province, China  
Yi Lei Huanggang City Center for Disease Control and Prevention, Huanggang 438000, Hubei Province, China  
Zeng Yang Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, College of Biology and Agricultural Resources, Huanggang Normal University, Huanggang 438000, Hubei Province, China  
Shi Yushuang Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, College of Biology and Agricultural Resources, Huanggang Normal University, Huanggang 438000, Hubei Province, China  
Fu Yue Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, College of Biology and Agricultural Resources, Huanggang Normal University, Huanggang 438000, Hubei Province, China  
Chen Zuwen Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, College of Biology and Agricultural Resources, Huanggang Normal University, Huanggang 438000, Hubei Province, China zuwenchen@hgnu.edu.cn 
Abstract:To investigate the virulence of a Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) to fall armyworm Spodoptera frugiperda and its infectious characteristics in vivo and in vitro, bioassays were conducted to determine the virulence of SfMNPV in the laboratory. Histopathological analysis of S. frugiperda infected by SfMNPV was carried out by using hematoxylin-eosin (HE) staining. Real-time fluorescence quantitative PCR was used to analyze the replication characteristics of SfMNPV in S. frugiperda larvae and Sf9 cells. The results showed that the LC50 and LT50 of SfMNPV to the second-instar larvae of S. frugiperda were 7.39×105 OBs/mL and 3.82 d, respectively. The epidermis, trachea, fat body, and midgut tissues of the larvae could be infected by SfMNPV, while muscle tissue was not significantly infected. The genome copies of SfMNPV in S. frugiperda larvae reached a maximum of 3.91×106 copies/ng at 96 h post-infection. The genome copies of SfMNPV in the Sf9 cell culture medium supernatant peaked at 2.08×107 copies/mL at 96 h post-infection.
keywords:Spodoptera frugiperda  baculovirus  insecticidal virulence  infectious characteristics  tissue
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