| 玉米灰斑病抗性鉴定技术 |
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| 引用本文：徐秀德,董怀玉,姜钰,刘志恒.玉米灰斑病抗性鉴定技术.植物保护学报,2003,30(2):129-132 |
| DOI：10.13802/j.cnki.zwbhxb.2003.02.004 |
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| 中文摘要:对玉米灰斑病菌孢子产生、病菌接种和寄主抗病性测定技术的研究结果表明,应用玉米叶粉碳酸钙琼脂和玉米叶粉琼脂两种培养基,温度24~25℃,培养5天,病菌可大量产生分生孢子;于植株喇叭口期,用注射器将病菌孢子悬浮液注射于植株喇叭口中,获得了理想的发病效果;对20个玉米自交系注射接种鉴定结果表明,玉米自交系间抗病性差异明显,但未发现免疫自交系。 |
| 中文关键词:玉米灰斑病 接种技术 抗病性鉴定 |
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| The technique for identification of resistance to gray leaf spot on maize |
| Author Name | Affiliation | | Xu Xiude | Institute of Plant Protection, Liaoning Academy of Agricultural Sciences, Shenyang 110161, China | | Dong Huaiyu | Institute of Plant Protection, Liaoning Academy of Agricultural Sciences, Shenyang 110161, China | | Jiang Yu | Institute of Plant Protection, Liaoning Academy of Agricultural Sciences, Shenyang 110161, China | | Liu Zhiheng | Shenyang Agricultural University, Shenyang 110161, China |
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| Abstract:Conidial production,inoculation and the pathogenicity test of Cercospora zeae-maydis Tehon&Daiels on maize were studied.The result showed that the pathogen on medium of maize leaf powder agar (MLPA) or maize leaf powder plus CaCO3 agar (MLPCA),cultured 5 days at 24-25℃,was found to produce abundant quantities of conidia.Inocula was inoculated into leaf whorl of plants by a hypodermic syringe at 9-11 leaf stage of plant.The increased disease incidence was observed in the fields.Identification of 20 inbred of maize for disease resistance showed that there was significantly different resistance to Cercospora zeae-maydis,but none was immune to the disease. |
| keywords:gray leaf spot of maize technique of inoculation identification of resistance |
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