江苏省建兰齿兰环斑病毒的鉴定和遗传多样性分析 Click here to download the full text Citation：吴伟文,贺振,刘娴,李良俊.江苏省建兰齿兰环斑病毒的鉴定和遗传多样性分析.Journal of Plant Protection,2019,46(4):745-753 Hits: Download times: Author Name Affiliation E-mail Wu Weiwen  School of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, Jiangsu Province, China    He Zhen  School of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, Jiangsu Province, China  hezhen@yzu.edu.cn  Liu Xian  School of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, Jiangsu Province, China    Li Liangjun  School of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, Jiangsu Province, China    中文摘要:为防控江苏省建兰上的齿兰环斑病毒（Odontoglossum ring spot virus，ORSV），采用酶联免疫吸附反应（enzyme-linked immuno sorbent assay，ELISA）和反转录-聚合酶链锁反应（reverse transcription-polymerase chain reaction，RT-PCR）对采自江苏省扬州市的16份具有典型感染ORSV病害症状的建兰叶片样品进行检测鉴定，并结合GenBank中已报道的相关序列对其进行多样性和系统发育树分析。结果显示，扬州市建兰上ORSV检出率高达100%；所获得的ORSV分离物与GenBank中其它141个分离物的核苷酸序列一致率均在94%以上，所有ORSV分离物只能形成1个分组，且组内多样性水平低，不具备典型的寄主和地理特异性；6个ORSV种群间差异较小，种群多态性均较低，其中ORSV印度尼西亚种群的种群多态性最高，为0.025，ORSV德国种群和ORSV印度尼西亚种群之间的遗传距离最高，为0.0244。表明不同地区、不同寄主种群ORSV分离物的遗传差异很小，种群内不同分离物有很高的相似性。 中文关键词:建兰  齿兰环斑病毒  序列分析  系统发育分析   Identification and genetic diversity analysis of Odontoglossum ring spot virus in Cymbidium ensifolium in Jiangsu Province Author Name Affiliation E-mail Wu Weiwen  School of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, Jiangsu Province, China    He Zhen  School of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, Jiangsu Province, China  hezhen@yzu.edu.cn  Liu Xian  School of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, Jiangsu Province, China    Li Liangjun  School of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, Jiangsu Province, China    Abstract:In order to prevent and control Odontoglossum ring spot virus (ORSV) in Cymbidium ensifolium in Jiangsu Province, 16 leaf samples collected from Yangzhou City were detected by enzymelinked immuno sorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR), and their diversity and phylogenetic analysis were combined with the related sequences reported in GenBank. The detection rate of ORSV on C. ensifolium was 100%. Sequence analysis showed that the ORSV isolates obtained in this study and the other 141 isolates in GenBank shared over 94% similarity. The phylogenetic analysis showed that ORSV isolates clustered into one group with low diversity and did not possess typical host and geographic specificity. Low population polymorphism was also found among the different ORSV geographical populations, and the Indonesia population had the highest value (0.025). The small genetic differences of ORSV among different geographical populations were obvious, and the highest value (0.0244) was found between Germany and Indonesia populations. In summary, the genetic diversity of different host populations in different ORSV regions was very small, and there were high similarities between different isolates within the population. keywords:Cymbidium ensifolium  Odontoglossum ring spot virus  sequence analysis  phylogenetic analysis View Full Text  View/Add Comment  Download reader

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