莲草直胸跳甲气味结合蛋白鉴定及其在触角中的表达分析 Click here to download the full text Citation：胡军,付丽,张彬,刘艳红,贾栋,马瑞燕.莲草直胸跳甲气味结合蛋白鉴定及其在触角中的表达分析.Journal of Plant Protection,2019,46(4):849-859 Hits: Download times: Author Name Affiliation E-mail Hu Jun  College of Life Sciences, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Fu Li  College of Agriculture, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Zhang Bin  College of Horticulture, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Liu Yanhong  College of Agriculture, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Jia Dong  College of Agriculture, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Ma Ruiyan  College of Agriculture, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China  maruiyan2004@163.com  中文摘要:为阐明莲草直胸跳甲Agasicles hygrophila对其寄主植物喜旱莲子草Alternanthera philoxeroides的专一性识别过程中气味结合蛋白（odorant-binding protein，OBP）的作用，基于莲草直胸跳甲三代全长转录组测序结果筛选获得其所有OBP基因并进行生物信息学分析，以最大似然法构建莲草直胸跳甲OBP的系统进化树，并通过定量PCR分析这些OBP基因在其触角中的表达量。结果表明，全长转录组测序结果中共鉴定得到53个莲草直胸跳甲AhygOBP基因，所编码蛋白有36个属于Minus-C家族，11个属于Classic家族，5个属于Plus-C家族，1个属于Atypical家族。进化分析结果显示，大部分莲草直胸跳甲AhygOBP与其它目昆虫OBP为直系同源。莲草直胸跳甲AhygOBP基因在触角中的表达分析结果显示，将表达量最低的AhygOBP31作为基准1，表达量最高的依次为AhygOBP53、AhygOBP32、AhygOBP13、AhygOBP28、AhygOBP44、AhygOBP45、AhygOBP29、Ahyg-OBP37、AhygOBP24，其中AhygOBP53的表达量是AhygOBP31的106 000倍。推测这些触角中高表达的AhygOBP可能在宿主植物识别中发挥重要作用。 中文关键词:莲草直胸跳甲  全长转录组测序  气味结合蛋白  触角  表达   Identification of odorant-binding proteins and their expression in the antennae of alligator weed flea beetle Agasicles hygrophila Author Name Affiliation E-mail Hu Jun  College of Life Sciences, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Fu Li  College of Agriculture, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Zhang Bin  College of Horticulture, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Liu Yanhong  College of Agriculture, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Jia Dong  College of Agriculture, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Ma Ruiyan  College of Agriculture, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China  maruiyan2004@163.com  Abstract:Alligator weed flea beetle, Agasicles hygrophila, is a special natural enemy of the global malignant invasive weed Alternanthera philoxeroides. Odorant-binding proteins (OBP) plays an important role in insect host-seeking. To identify the olfactory mechanisms underlying host preference and localization, the OBP genes of A. hygrophila were obtained from the results and analysis of the previous three-generation full-length transcriptome sequencing. Moreover, the expression levels of these OBPs in the antennae were determined by real time quantitative PCR. The results showed that 53 AhygOBP genes were identified in the full-length transcriptome sequencing. Among them, 36 of the encoded proteins belonged to Minus-C family, 11 to Classic family, five to Plus-C family, and one to Atypical family. Phylogenetic analysis revealed that most AhygOBPs were orthologous to other insect OBPs. The lowest expression level of AhygOBP31 was set to 1, AhygOBP53, AhygOBP32, AhygOBP13, AhygOBP28, AhygOBP44, AhygOBP45, AhygOBP29, AhygOBP37, AhygOBP24 had high expression, of which AhygOBP53 was the highest, which was 106 000 times of AhygOBP31. It indicated that high expression of OBPs in antennae might play an important role in host plant recognition. keywords:Agasicles hygrophila  full-lengthtranscriptome sequencing  odorant-binding protein  antenna  expression View Full Text  View/Add Comment  Download reader

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