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四川省眉山市六个柑橘品种上衰退病毒分离株的组群构成、基因型及遗传特征
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引用本文:钟可,易龙,周俊,陈波,黄爱军.四川省眉山市六个柑橘品种上衰退病毒分离株的组群构成、基因型及遗传特征.植物保护学报,2021,48(4):732-741
DOI:10.13802/j.cnki.zwbhxb.2021.2020116
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作者单位E-mail
钟可 赣南师范大学生命科学学院, 江西 赣州 341000  
易龙 赣南师范大学生命科学学院, 江西 赣州 341000 yilongswu@163.com 
周俊 赣南师范大学生命科学学院, 江西 赣州 341000  
陈波 赣南师范大学地理与环境工程学院, 江西 赣州 341000  
黄爱军 赣南师范大学生命科学学院, 江西 赣州 341000  
中文摘要:为有效利用弱毒株系交叉保护(mild strain cross protection,MSCP)防控柑橘衰退病,分别采用限制性片段长度多态性(restriction fragment length polymorphism,RFLP)、双向逆转录-聚合酶链式反应(bi-directional reverse transcription polymerase chain reaction,BD-PCR)及多重分子标记对四川省眉山市6个柑橘品种30个样品上的柑橘衰退病毒(citrus tristeza virus,CTV)分离株进行组群构成、基因型、遗传特征和系统发育树分析。结果表明,在30个柑橘样品中,70.0%的样品为多种CTV组群混合侵染,且多为强毒和弱毒株系混合侵染;6个柑橘品种上CTV组群构成存在差异,温州蜜柑品种主要为组群1和组群3混合侵染,纽荷尔脐橙品种均为组群3和组群5混合侵染,其他4个品种主要为组群3和组群5混合侵染和组群3单一侵染。6个柑橘品种上CTV分离株均由3~5种基因型构成,构成较相似,均包括T3、T30、T36三种基因型,而不知火品种上未检出B165基因型。6个柑橘品种上CTV分离株的核苷酸序列相似性较高,介于92.4%~99.8%之间;遗传分化系数介于-0.114~0.467,基因流介于-35.89~52.92之间,其中纽荷尔脐橙CTV种群与除沃柑外其他品种的CTV种群的分化系数均大于0.281,基因流均小于1.00,表明这6个柑橘品种CTV种群之间存在基因交流,但纽荷尔脐橙CTV种群与其他品种交流不频繁;所有CTV分离株均被聚为3个不同的类群,其中春见、蜜柑、爱媛品种上大部分CTV分离株被聚为类群I,纽荷尔脐橙品种上所有CTV分离株被聚为类群II,而沃柑、不知火品种上大部分CTV分离株被聚为类群III;CTV种群间变异百分比为80.86%,表明寄主品种是CTV分离株p25基因遗传分化的重要因素。
中文关键词:柑橘衰退病毒  p25基因  组群构成  基因型  序列分析
 
Group composition, genotype and genetic characteristics of citrus tristeza virus isolates from six citrus cultivars in Meishan, Sichuan Province
Author NameAffiliationE-mail
Zhong Ke School of Life Science, Gannan Normal University, Ganzhou 341000, Jiangxi Province, China  
Yi Long School of Life Science, Gannan Normal University, Ganzhou 341000, Jiangxi Province, China yilongswu@163.com 
Zhou Jun School of Life Science, Gannan Normal University, Ganzhou 341000, Jiangxi Province, China  
Chen Bo School of Geography and Environmental Engineering, Gannan Normal University, Ganzhou 341000, Jiangxi Province, China  
Huang Aijun School of Life Science, Gannan Normal University, Ganzhou 341000, Jiangxi Province, China  
Abstract:In order to effectively use the mild strain cross protection (MSCP) to prevent and control the citrus tristeza virus (CTV) disease, and to understand the differences in the group composition, genotype and genetic characteristics of CTV isolates from different citrus varieties in the same area, the p25 gene of 30 CTV isolates from six citrus varieties in Meishan, Sichuan were analyzed with restriction fragment length polymorphism, multiple molecular markers, genetic diversity, and phylogenetic analysis in this study. Group composition analysis showed that 70.0% of CTV isolates in 30 citrus samples were infected by multiple CTV groups, showing mixed infection of virulent and mild strains. There were differences in the CTV group composition among the six citrus varieties. Satsuma mandarin varieties were mainly mixedly infected with Groups 1 and 3, and Newhall navel orange varieties were mixedly infected with Groups 3 and 5, while the other four varieties were mainly mixedly infected with Groups 3 and 5 or singly infected with Group 3. Genotyping analysis showed that 30 CTV isolates from six citrus varieties were composed of 3-5 genotypes, all of which included T3, T30 and T36 genotypes, while no virulent strain B165 was detected in Shiranui varieties. The genetic diversity analysis of CTV p25 gene showed that the nucleotide sequence similarity among the six varieties was 92.4%-99.8%. The genetic differentiation coefficient was -0.114-0.467 and the gene flow was -35.89-52.92. The genetic differentiation coefficients of Newhall navel orange CTV populations with other varieties (except Orah CTV population) were greater than 0.281, with the gene flows less than 1.00, indicating that there was gene exchange between CTV isolate populations of the six varieties, but the gene exchange between Newhall navel orange CTV populations and other varieties was not frequent. Phylogenetic analysis showed that the all isolates were clustered into three different groups. Most of the CTV isolates from the varieties Harumi, Satsuma mandarin, and Aiyuan and five representative stem pitting virulent strains were clustered in group Ⅰ, and all CTV isolates from Newhall navel orange and T3 virulent strains were clustered in group Ⅱ, while most CTV isolates from the varieties Orah and Shiranui and virulent and mild strains were clustered in group Ⅲ. The variation between CTV populations on different varieties accounted for 80.86%, indicating that the differentiation of CTV populations in the same area was mainly affected by the different host varieties. Key words:citrus tristeza virus; p25 gene; the group composition; genotype; sequence analysis
keywords:citrus tristeza virus  p25 gene  the group composition  genotype  sequence analysis
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