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瓠瓜果斑病症状及其病原菌的鉴定
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引用本文:罗群,李艳敏,林钗,孙超,高其康,楼兵干.瓠瓜果斑病症状及其病原菌的鉴定.植物保护学报,2021,48(4):748-756
DOI:10.13802/j.cnki.zwbhxb.2021.2020138
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作者单位E-mail
罗群 浙江大学生物技术研究所, 农业农村部作物病虫分子生物学重点实验室, 杭州 310058  
李艳敏 浙江省植保检疫与农药管理总站, 杭州 310020  
林钗 浙江省农业教育培训中心, 杭州 310029  
孙超 浙江大学农生环测试中心, 杭州 310058  
高其康 浙江大学农生环测试中心, 杭州 310058  
楼兵干 浙江大学生物技术研究所, 农业农村部作物病虫分子生物学重点实验室, 杭州 310058 bglou@zju.edu.cn 
中文摘要:为有效防控瓠瓜果斑病,自浙江省象山县田间采集具有典型果斑病症状的瓠瓜样本,对其进行病原菌分离、形态观察、致病性测定及分子生物学鉴定,并利用特异性引物PL1/PL2 PCR扩增和基质辅助激光解吸电离飞行时间质谱(matrix assisted laser desorption ionization time-of-flight massspectrometry,MALDI-TOF-MS)技术对其病原菌进行亚群鉴定。结果表明:瓠瓜果斑病田间典型症状是发病叶片和果实上病斑由水渍状小斑点逐渐发展为伴有黄色晕圈的褐色不规则病斑,果实腐烂、有臭味。通过菌体形态和培养特性观察、PCR鉴定、16S rDNA序列分析和7个看家基因的系统发育分析将其病原菌鉴定为西瓜噬酸菌Acidovorax citrulli。从瓠瓜上分离的菌株均属于西瓜噬酸菌亚群I,从西瓜上分离的菌株均属于西瓜噬酸菌亚群II。
中文关键词:瓠瓜  西瓜噬酸菌  16S rDNA  看家基因  基质辅助激光解吸电离飞行时间质谱
 
Symptoms of fruit blotch in bottle gourd and identification of its pathogen
Author NameAffiliationE-mail
Luo Qun Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture and Rural Affairs
Institute of Biotechnology, Zhejiang University, Hangzhou 310058, Zhejiang Province, China 
 
Li Yanmin Zhejiang General Station of Plant Protection Quarantine and Agrochemicals Management, Hangzhou 310020, Zhejiang Province, China  
Lin Chai Zhejiang Agricultural Education & Training Center, Hangzhou 310029, Zhejiang Province, China  
Sun Chao Analysis Center of Agrobiology and Environmental Sciences, Zhejiang University, Hangzhou 310058, Zhejiang Province, China  
Gao Qikang Analysis Center of Agrobiology and Environmental Sciences, Zhejiang University, Hangzhou 310058, Zhejiang Province, China  
Lou Binggan Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture and Rural Affairs
Institute of Biotechnology, Zhejiang University, Hangzhou 310058, Zhejiang Province, China 
bglou@zju.edu.cn 
Abstract:Aiming to effectively prevent and control fruit blotch of bottle gourd (Lagenaria siceraria), samples of bottle gourd with typical symptoms of fruit blotch were collected from fields in Xiangshan County, Zhejiang Province, pathogen was isolated from these samples, and then morphological observation, pathogenicity test and molecular biological identification were carried out. The group of pathogens were also identified via PCR amplification with specific primers PL1/PL2 and matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) technology. The results showed that the typical symptoms of the disease included spots on the infected leaves and fruits, which gradually developed from small water-like spots into brown irregular spots with yellow halos, and caused the fruit to rot and smell bad. Bacterial morphology, culture characteristics, PCR identification, 16S rDNA sequencing and phylogenetic analysis based on seven housekeeping genes confirmed that the pathogen was Acidovorax citrulli. All the strains isolated from bottle gourd were A. citrulli group I while those from watermelon belonged to A. citrulli group II.
keywords:bottle gourd  Acidovorax citrulli  16S rDNA  housekeeping gene  MALDI-TOF-MS
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