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青海省青稞根腐病调查及病原菌鉴定
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引用本文:李雪萍,许世洋,汪学苗,张怡忻,范雨轩,漆永红,李敏权.青海省青稞根腐病调查及病原菌鉴定.植物保护学报,2021,48(4):757-765
DOI:10.13802/j.cnki.zwbhxb.2021.2020135
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作者单位E-mail
李雪萍 甘肃省农业科学院植物保护研究所, 兰州 730070  
许世洋 甘肃农业大学草业学院, 兰州 730070  
汪学苗 甘肃农业大学草业学院, 兰州 730070  
张怡忻 甘肃农业大学草业学院, 兰州 730070  
范雨轩 甘肃农业大学草业学院, 兰州 730070  
漆永红 甘肃省农业科学院植物保护研究所, 兰州 730070  
李敏权 甘肃省农业科学院植物保护研究所, 兰州 730070 liminquan@gsagr.ac.cn 
中文摘要:为明确青海省青稞根腐病发生情况及其病原菌,于2016年6—9月对青海省海北藏族自治州海晏县、刚察县,海东市互助土族自治县苗期及成株期青稞上根腐病发生情况进行调查,采集19份根腐病样品,采用常规组织分离法对样品进行分离,并对分离菌株进行致病性测定,通过形态学特征及分子生物学方法对其进行鉴定。结果显示,青海省青稞根腐病分布广泛,发病率较高,为10%~20%;发病植株长势弱、发黄,茎基部缢缩腐烂,穗白粒瘪,茎秆发褐或黑红;19份样品中共分离纯化得到93株病原菌菌株,其中73株为燕麦镰孢Fusarium avenaceum,9株为木贼镰孢F.equiseti,11株为锐顶镰孢F.acuminatum;木贼镰孢的绝对病情指数最强,为79.43,燕麦镰孢次之,为50.90,锐顶镰孢最弱,为23.70,各病原种间及种内致病性差异显著。根据分离率及各病原菌致病性综合确定燕麦镰孢为青海省青稞根腐病的优势病原菌。
中文关键词:青稞  根腐病  燕麦镰孢  致病性  病原菌鉴定
 
Field survey and pathogen identification of naked barley root rot in Qinghai Province
Author NameAffiliationE-mail
Li Xueping Institute of Plant Protection, Gansu Academy of Agricultural Sciences, Lanzhou 730070, Gansu Province, China  
Xu Shiyang College of Prataculture, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China  
Wang Xuemiao College of Prataculture, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China  
Zhang Yixin College of Prataculture, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China  
Fan Yuxuan College of Prataculture, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China  
Qi Yonghong Institute of Plant Protection, Gansu Academy of Agricultural Sciences, Lanzhou 730070, Gansu Province, China  
Li Minquan Institute of Plant Protection, Gansu Academy of Agricultural Sciences, Lanzhou 730070, Gansu Province, China liminquan@gsagr.ac.cn 
Abstract:In order to clarify the occurrence and etiology of naked barley root rot in Qinghai Province, field surveys were conducted on naked barley root rot at seedling and adult stage, and 19 diseased root samples were collected. Isolates were obtained from these samples using the conventional tissue separation method, their pathogenicity was determined. The identification of these isolates was done based on their morphological characteristics and phylogenetic analysis of their TEF-1α gene sequences. The results showed that root rot was widely distributed in naked barley fields surveyed with the incidence ranging from about 10% to 20%. The diseased plants grown weak and leaves turned yellow, other symptoms also included constricted and rotten stem bases, the white ears, hollow grains, and brown or black red stems. A total of 93 isolates were identified as Fusarium, of which 73 were F. avenaceum, nine were F. equiseti and 11 were F. acuminatum. Pathogenicity test showed that F. equiseti was the most aggressive with a disease index of 79.43, followed by F. avenaceum with a disease index of 50.90, while F. acuminatum had the lowest disease index of 23.70. Significant difference was detected in interspecific crossing and intraspecific. Considering both isolation rate and pathogenicity, it was found that F. avenaceum was the dominant pathogen of naked barley fusarium root rot in Qinghai Province.
keywords:naked barley  root rot  Fusarium avenaceum  pathogenicity  pathogen identification
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