葡萄白腐病菌对抑霉唑的敏感基线及其与不同杀菌剂的交互抗性 Click here to download the full text Citation：李宝燕,石洁,田园园,聂乐兴,王英姿.葡萄白腐病菌对抑霉唑的敏感基线及其与不同杀菌剂的交互抗性.Journal of Plant Protection,2021,48(4):774-780 Hits: Download times: Author Name Affiliation E-mail Li Baoyan  Yantai Academy of Agricultural Sciences, Yantai 265500, Shandong Province, China    Shi Jie  Yantai Academy of Agricultural Sciences, Yantai 265500, Shandong Province, China    Tian Yuanyuan  Yantai Academy of Agricultural Sciences, Yantai 265500, Shandong Province, China College of Life Sciences, Yantai University, Yantai 264005, Shandong Province, China    Nie Lexing  Bayer Crop Science (China) Company Ltd., Yantai 265700, Shandong Province, China    Wang Yingzi  Yantai Academy of Agricultural Sciences, Yantai 265500, Shandong Province, China College of Life Sciences, Yantai University, Yantai 264005, Shandong Province, China  ytnkyzbs@126.com  中文摘要:为明确山东省胶东地区葡萄白腐病菌Coniella diplodiella对抑霉唑的敏感性及抑霉唑与常规杀菌剂之间的交互抗性，采用菌丝生长速率法测定葡萄白腐病菌对抑霉唑等杀菌剂的敏感性，并通过分析抑霉唑与戊唑醇、吡唑醚菌酯、福美双、多菌灵、代森锰锌毒力的相关性，判断抑霉唑与各药剂之间是否存在交互抗性。结果表明，供试69株葡萄白腐病菌菌株对抑霉唑的EC50在0.13~55.53 μg/mL之间，最高值与最低值相差427.15倍；其频数分布图呈多峰曲线，第一主峰内EC50平均值为6.34 μg/mL，可作为胶东地区葡萄白腐病菌对抑霉唑的敏感基线。与敏感基线相比，田间已出现抑霉唑低抗菌株，占检测总株数的8.70%，未检测到中、高抗菌株。选择3个抗性菌株连续继代培养10代后，其对抑霉唑的敏感性明显提高，说明其抗药性不能稳定遗传。抑霉唑EC50对数值与戊唑醇、吡唑醚菌酯、福美双、多菌灵、代森锰锌的EC50对数值之间的相关系数分别为0.799、-0.143、-0.089、-0.268和0.159，说明抑霉唑与戊唑醇存在一定的交互抗性，但与其他4种药剂之间不存在交互抗性。表明抑霉唑可用于胶东地区田间葡萄白腐病的有效防控。 中文关键词:葡萄白腐病菌  抑霉唑  敏感基线  交互抗性   The sensitivity to imazalil and cross-resistance against several other fungicides in grapevine white rot pathogen Coniella diplodiella Author Name Affiliation E-mail Li Baoyan  Yantai Academy of Agricultural Sciences, Yantai 265500, Shandong Province, China    Shi Jie  Yantai Academy of Agricultural Sciences, Yantai 265500, Shandong Province, China    Tian Yuanyuan  Yantai Academy of Agricultural Sciences, Yantai 265500, Shandong Province, China College of Life Sciences, Yantai University, Yantai 264005, Shandong Province, China    Nie Lexing  Bayer Crop Science (China) Company Ltd., Yantai 265700, Shandong Province, China    Wang Yingzi  Yantai Academy of Agricultural Sciences, Yantai 265500, Shandong Province, China College of Life Sciences, Yantai University, Yantai 264005, Shandong Province, China  ytnkyzbs@126.com  Abstract:To evaluate the sensitivity to imazalil and the cross-resistances with tebuconazole, pyraclostrobin, thiram, carbendazim, and mancozeb, in isolates of grapevine white rot pathogen Coniella diplodiella collected from Jiaodong, Shandong Province, the EC50 of these isolates to each of the six fungicides was determined using the mycelial growth rate method and correlation analysis was conducted between the EC 50 of paired fungicides. The results showed that the EC50 of 69 C. diplodiella isolates to imazalil ranged from 0.13 μg/mL to 55.53 μg/mL with a 427.15 fold difference between the highest and lowest values. The frequency distribution of EC50 showed a multiple peak curve, and the average value in the first peak was 6.34 μg/mL, which was used as a sensitivity baseline of C. diplodiella to imazalil in the subsequently analyses. The results from the sensitivity test revealed that 8.70% of the total tested strains had a low imazalil-resistance. No isolate was moderately or highly imazalil-resistant. The sensitivity of three resistant strains to imazalil increased significantly after ten generations of continuous subculture, indicating that the resistance to imazalil could not be inherited stably. The correlation coefficients were 0.799, -0.143, -0.089, -0.268 and 0.159, respectively, in linear regression analysis between the logarithms of EC50 values of imazole and tebuconazole, pyraclostrobin, thiram, carbendazim and mancozeb, which indicated that there was moderate cross resistance between imazole and tebuconazole, but no cross resistance between imazalil and other four fungicides. The results indicated that imazalil could be used for effective control of grape white rot in the orchards. keywords:Coniella diplodiella  imazalil  baseline-sensitivity  cross-resistance View Full Text  View/Add Comment  Download reader

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