香茅精油微乳液对朱砂叶螨的生物活性及其解毒酶系活性的影响 Click here to download the full text Citation：程作慧,樊芳芳,王旗,刘庆,张鹏,李锐,李生才.香茅精油微乳液对朱砂叶螨的生物活性及其解毒酶系活性的影响.Journal of Plant Protection,2021,48(4):914-920 Hits: Download times: Author Name Affiliation E-mail Cheng Zuohui  College of Basic Department, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China  501191438@qq.com  Fan Fangfang  College of Basic Department, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Wang Qi  College of Basic Department, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Liu Qing  College of Basic Department, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Zhang Peng  College of Basic Department, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Li Rui  College of Agriculture, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Li Shengcai  College of Agriculture, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China  sxaulisc@126.com  中文摘要:为明确香茅精油微乳液对朱砂叶螨的生物活性及其解毒酶系活性的影响，采用熏蒸法测定香茅精油微乳液对朱砂叶螨的熏蒸活性及香茅精油微乳液亚致死剂量处理下朱砂叶螨的产卵情况、趋避情况及解毒酶系活性。结果表明，当剂量达到2.5 μL时，香茅精油微乳液处理下朱砂叶螨的死亡率达到80.37%以上；香茅精油微乳液熏蒸24 h时，其对朱砂叶螨的亚致死剂量LD10和LD30分别为0.082 μL和0.284 μL；香茅精油微乳液LD10和LD30处理24 h时，其对朱砂叶螨的产卵抑制率分别为76.30%和78.67%，对朱砂叶螨的趋避率分别为80.00%和88.00%；香茅精油微乳液LD10和LD30处理后，朱砂叶螨体内酯酶活性在0~8 h表现出激活作用，随后一直处于抑制状态，谷胱甘肽-S-转移酶活性一直处于激活状态，而多功能氧化酶活性处于抑制状态。表明香茅精油微乳液是一种很有前景的绿色杀螨剂。 中文关键词:香茅精油  微乳液  朱砂叶螨  熏蒸活性  酶   Bioactivities of citronella oil microemulsion against Tetranychus cinnabarinus (Acari: Tetranychidae) and its effect on the detoxification enzyme activities Author Name Affiliation E-mail Cheng Zuohui  College of Basic Department, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China  501191438@qq.com  Fan Fangfang  College of Basic Department, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Wang Qi  College of Basic Department, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Liu Qing  College of Basic Department, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Zhang Peng  College of Basic Department, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Li Rui  College of Agriculture, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Li Shengcai  College of Agriculture, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China  sxaulisc@126.com  Abstract:To clarify the bioactivities of citronella oil microemulsion against carmine spider mite Tetranychus cinnabarinus and its effect on the activity of detoxification enzymes, the fumigation activity of citronella oil microemulsion and the oviposition inhibitory activity, repellent activity and detoxification enzyme activity at the sublethal dosages of citronella oil microemulsion against T. cinnabarinus were determined using the fumigation method. The results showed that the mortality of T. cinnabarinus caused by citronella oil microemulsion reached more than 80.37% at the dosage of 2.5 μL. The sublethal dosages of LD10 and LD30 were 0.082 μL and 0.284 μL of citronella oil microemulsion by fumigating T. cinnabarinus for 24 h. By fumigating with citronella oil microemulsion at LD10 and LD30 for 24 h, the oviposition inhibition rates were 76.30% and 78.67%, and the repellency rates were 80.00% and 88.00%, respectively. The esterase activity was up-regulated in eight hours but then down-regulated; the glutathione-S-transferase activity was up-regulated but the mixed function oxidase activity was downregulated when treated with citronella oil microemulsion at LD10 and LD30. It indicated that citronella oil microemulsion was a promising green acaricide. keywords:citronella oil  microemulsion  Tetranychus cinnabarinus  fumigation activity  enzyme View Full Text  View/Add Comment  Download reader

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