云南省不同海拔区草地贪夜蛾幼虫肠道与粪便细菌多样性 Click here to download the full text Citation：李永萍,杜广祖,徐天梅,杨航,杨进波,张庭发,易小光,陈斌.云南省不同海拔区草地贪夜蛾幼虫肠道与粪便细菌多样性.Journal of Plant Protection,2022,49(2):559-573 Hits: Download times: Author Name Affiliation E-mail LI Yong-ping  State Key Laboratory for Conservation and Utilization of Bioresources in Yunnan, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, Yunnan Province, China    DU Guang-zu  State Key Laboratory for Conservation and Utilization of Bioresources in Yunnan, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, Yunnan Province, China    XU Tian-mei  State Key Laboratory for Conservation and Utilization of Bioresources in Yunnan, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, Yunnan Province, China    YANG Hang  Yunnan Green Biotechnology Science and Technology Co. Ltd., Midu 675600, Yunnan Province, China    YANG Jin-bo  Yunnan Green Biotechnology Science and Technology Co. Ltd., Midu 675600, Yunnan Province, China    ZHANG Ting-fa  Yunnan Green Biotechnology Science and Technology Co. Ltd., Midu 675600, Yunnan Province, China    YI Xiao-guang  Yunnan Green Biotechnology Science and Technology Co. Ltd., Midu 675600, Yunnan Province, China    CHEN Bin  State Key Laboratory for Conservation and Utilization of Bioresources in Yunnan, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, Yunnan Province, China  chbins@163.com  中文摘要:为探明草地贪夜蛾Spodoptera frugiperda不同海拔种群的幼虫肠道及粪便细菌组成，采用分离培养法、形态特征及16S rDNA测序分析法对云南省5个海拔22个草地贪夜蛾种群的幼虫肠道及粪便细菌进行分离培养、鉴定。结果表明，从不同海拔种群的幼虫肠道和粪便中分别获得可培养细菌4门15科22属45种和4门13科18属36种，优势菌门均为变形菌门和厚壁菌门。不同海拔种群的幼虫肠道和粪便细菌组成均不同，500 m以下、1 001~1 500 m、1 501~2 000 m和2 000 m以上4个海拔种群的幼虫肠道细菌优势属和优势种分别为肠球菌属Enterococcus和铅黄肠球菌Enterococcus casseliflavus，500~1 000 m海拔种群的优势属和优势种分别为克雷伯氏菌属Klebsiella和变栖克雷伯氏菌Klebsiella variicola； 500 m以下、500~1 000 m、1 001 m以上海拔种群的幼虫粪便细菌优势属分别为假单胞菌属Pseudomonas、克雷伯氏菌属和肠球菌属，优势种分别为韩国假单胞菌Pseudomonas koreensis、变栖克雷伯氏菌和铅黄肠球菌。5个海拔种群的幼虫肠道和粪便细菌的Shannon-Weiner指数、Simpson指数和Pielou均匀度指数分别为0.35~3.42和1.04~3.38、0.09~0.86和0.48~0.84、0.22~1.17和0.50~1.23，其中1 501~2 000 m海拔种群的幼虫肠道和粪便细菌的ShannonWeiner指数、Simpson指数和Pielou均匀度指数均最高。表明不同海拔种群草地贪夜蛾幼虫肠道及粪便细菌群落组成差异显著。 中文关键词:草地贪夜蛾  肠道细菌  粪便细菌  海拔  云南省   Diversity of intestinal and fecal bacteria in fall armyworm Spodoptera frugiperda larvae from different altitudes of Yunnan Province Author Name Affiliation E-mail LI Yong-ping  State Key Laboratory for Conservation and Utilization of Bioresources in Yunnan, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, Yunnan Province, China    DU Guang-zu  State Key Laboratory for Conservation and Utilization of Bioresources in Yunnan, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, Yunnan Province, China    XU Tian-mei  State Key Laboratory for Conservation and Utilization of Bioresources in Yunnan, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, Yunnan Province, China    YANG Hang  Yunnan Green Biotechnology Science and Technology Co. Ltd., Midu 675600, Yunnan Province, China    YANG Jin-bo  Yunnan Green Biotechnology Science and Technology Co. Ltd., Midu 675600, Yunnan Province, China    ZHANG Ting-fa  Yunnan Green Biotechnology Science and Technology Co. Ltd., Midu 675600, Yunnan Province, China    YI Xiao-guang  Yunnan Green Biotechnology Science and Technology Co. Ltd., Midu 675600, Yunnan Province, China    CHEN Bin  State Key Laboratory for Conservation and Utilization of Bioresources in Yunnan, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, Yunnan Province, China  chbins@163.com  Abstract:To reveal the composition of intestinal and fecal bacteria of fall armyworm Spodoptera frugiperda from different geographical altitudes, the culturable bacteria in the intestines and faeces of S. frugiperda from 22 locations at different altitudes were isolated and cultured on media, and the species of bacteria were identified using the morphological characters and 16S rDNA sequences. The results showed that 45 species, 22 genera, 15 families in four phyla and 36 species, 18 genera, 13 families in four phyla were isolated from the intestines and faeces of larval S. frugiperda, respectively. The most common phyla were Proteobacteria and Firmicutes. The composition structures of intestinal and fecal bacteria of S. frugiperda larvae from different altitudes were different. The predominant bacterial genera and species were Enterococcus and Enterococcus casseliflavus in larvae from four altitudes, below 500 m, 1 001-1 500 m, 1 501-2 000 m and above 2 000 m; the most common genera and species of bacteria were Klebsiella and Klebsiella variicola in the larval intestines of S. frugiperda at the altitude of 500-1 000 m. The predominant fecal bacterial genera were Pseudomonas, Klebsiella and Enterococcus, and the predominant fecal bacterial species were Pseudomonas koreensis, Klebsiella variicola and Enterococcus casseliflavus in larvae from the altitudes of below 500 m, 500-1 000 m and above 1 001 m. The Shannon-Weiner, Simpson and Pielou evenness indexes of intestinal and fecal bacteria of S. frugiperda from five altitudes were 0.35-3.42 and 1.04-3.38, 0.09-0.86 and 0.48-0.84, 0.22-1.17 and 0.50- 1.23, respectively. The results suggested that there were significant differences in the bacterial community composition in the intestines and faeces of S. frugiperda larvae at different altitudes. keywords:Spodoptera frugiperda  intestinal bacteria  fecal bacteria  altitude  Yunnan Province View Full Text  View/Add Comment  Download reader

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