云南省稻瘟病菌群体的致病性分析及交配型分布
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Citation：董丽英,刘树芳,田维逵,周伍民,张先闻,李迅东,杨勤忠.云南省稻瘟病菌群体的致病性分析及交配型分布.Journal of Plant Protection,2023,50(2):316-324
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Author NameAffiliationE-mail
Dong Liying Key Laboratory of Green Prevention and Control of Agricultural Transboundary Pests of Yunnan Province, Institute of Agricultural Environment and Resource Research, Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan Province, China  
Liu Shufang Key Laboratory of Green Prevention and Control of Agricultural Transboundary Pests of Yunnan Province, Institute of Agricultural Environment and Resource Research, Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan Province, China  
Tian Weikui Plant Protection and Quarantine Station of Menghai County, Yunnan, Menghai 666200, Yunnan Province, China  
Zhou Wumin Key Laboratory of Green Prevention and Control of Agricultural Transboundary Pests of Yunnan Province, Institute of Agricultural Environment and Resource Research, Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan Province, China
Institute of Resource Plants Research, Yunnan University, Kunming 650091, Yunnan Province, China 		 
Zhang Xianwen Key Laboratory of Green Prevention and Control of Agricultural Transboundary Pests of Yunnan Province, Institute of Agricultural Environment and Resource Research, Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan Province, China
Institute of Resource Plants Research, Yunnan University, Kunming 650091, Yunnan Province, China 		 
Li Xundong Key Laboratory of Green Prevention and Control of Agricultural Transboundary Pests of Yunnan Province, Institute of Agricultural Environment and Resource Research, Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan Province, China  
Yang Qinzhong Key Laboratory of Green Prevention and Control of Agricultural Transboundary Pests of Yunnan Province, Institute of Agricultural Environment and Resource Research, Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan Province, China qzhyang@163.com 
中文摘要:为明确云南省不同稻区稻瘟病菌Magnaporthe oryzae的毒性频率及交配型分布,利用2007-2013年分离自云南省不同稻区的112株稻瘟病菌单孢菌株,对23个持有不同抗性基因的单基因系和持有Pi57(t)的水稻渗入系IL-E1454进行致病性测定。结果表明,稻瘟病菌对不同抗病基因的毒性频率存在很大差异,分离自粳稻区的稻瘟病菌菌株对持有Piz-t、Pi5、Pi9、Pi20和Pi57(t)这5个水稻品系的毒性频率分别为14.29%、5.36%、5.51%、5.36%和0;分离自籼稻区的稻瘟病菌菌株对持有Pik-h、Piz、Pita、Piz-5、Pita-2、Pi5、Pi7和Pi9这8个水稻品系的毒性频率分别为18.25%、9.13%、9.64%、7.50%、15.72%、0、13.05%和0;分离自陆稻区的稻瘟病菌菌株菌株对持有Pik-h、Pib、Pish、Pi1、Pi5、Pi9、Pi11和Pi57(t)这8个水稻品系的毒性频率分别为6.67%、3.33%、13.79%、13.33%、7.69%、6.67%、0和3.23%;交配型测定结果显示,陆稻区菌株可交配率为100.00%,其中MAT1-1和MAT1-2菌株分别为43.33%和56.67%;粳稻区菌株可交配率为91.84%,其中MAT1-1和MAT1-2菌株分别为69.39%和22.45%;籼稻区菌株可交配率为87.88%,其中MAT1-1和MAT1-2菌株分别为18.18%和69.70%,表明不同稻区稻瘟病菌的育性均比较高,但交配型分布极不平衡。
中文关键词:稻瘟病菌  致病性  毒性频率  交配型  云南省
 
Pathogenicity and mating type of rice blast fungus Magnaporthe oryzae isolates in Yunnan Province
Author NameAffiliationE-mail
Dong Liying Key Laboratory of Green Prevention and Control of Agricultural Transboundary Pests of Yunnan Province, Institute of Agricultural Environment and Resource Research, Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan Province, China  
Liu Shufang Key Laboratory of Green Prevention and Control of Agricultural Transboundary Pests of Yunnan Province, Institute of Agricultural Environment and Resource Research, Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan Province, China  
Tian Weikui Plant Protection and Quarantine Station of Menghai County, Yunnan, Menghai 666200, Yunnan Province, China  
Zhou Wumin Key Laboratory of Green Prevention and Control of Agricultural Transboundary Pests of Yunnan Province, Institute of Agricultural Environment and Resource Research, Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan Province, China
Institute of Resource Plants Research, Yunnan University, Kunming 650091, Yunnan Province, China 		 
Zhang Xianwen Key Laboratory of Green Prevention and Control of Agricultural Transboundary Pests of Yunnan Province, Institute of Agricultural Environment and Resource Research, Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan Province, China
Institute of Resource Plants Research, Yunnan University, Kunming 650091, Yunnan Province, China 		 
Li Xundong Key Laboratory of Green Prevention and Control of Agricultural Transboundary Pests of Yunnan Province, Institute of Agricultural Environment and Resource Research, Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan Province, China  
Yang Qinzhong Key Laboratory of Green Prevention and Control of Agricultural Transboundary Pests of Yunnan Province, Institute of Agricultural Environment and Resource Research, Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan Province, China qzhyang@163.com 
Abstract:In order to understand the virulence frequency and mating type distribution of rice blast fungusMagnaporthe oryzae in different rice-growing regions of Yunnan Province, 112 isolates of M. oryzae collected from different rice-growing regions of Yunnan from 2007 to 2013 were tested on 23 monogenic lines and the introgression line IL-E1454 carrying blast resistant gene Pi57(t). The results indicated that the frequency of M. oryzae isolates virulent to different rice lines showed significant differences. The frequency of M. oryzae isolates from japonica-rice-growing regions virulent to Piz-t, Pi5, Pi9, Pi20 and Pi57(t) were 14.29%, 5.36%, 5.51%, 5.36% and 0, respectively; the frequency of M. oryzae isolates from indica-rice-growing regions virulent to Pik-h, Piz, Pita, Piz-5, Pita-2, Pi5, Pi7 and Pi9 were 18.25%, 9.13%, 9.64%, 7.50%, 15.72%, 0, 13.05% and 0, respectively; the frequency of M. oryzae isolates from upland-rice-growing regions virulent to Pik-h, Pib, Pish, Pi1, Pi5, Pi9, Pi11 and Pi57(t) were 6.67%, 3.33%, 13.79%, 13.33%, 7.69%, 6.67%, 0 and 3.23%, respectively. Determination of mating type with four standard tested isolates showed that all strains from upland rice were fertile, and MAT1-1 and MAT1-2 isolates accounted for 43.33% and 56.67% of total isolates, respectively; the percentage of fertile isolates from japonica rice-growing regions was 91.84%, and MAT1-1 and MAT1-2 isolates accounted for 69.39% and 22.45%, respectively; the percentage of fertile isolates from indica rice-growing regions accounted for 87.88%, of them MAT1-1 and MAT1-2 isolates accounted for 18.18% and 69.70%, respectively. The fertility of M. oryzae isolates was high in different rice areas, but the distribution of their mating types was uneven in some populations.
keywords:Magnaporthe oryzae  pathogenicity  virulence frequency  mating type  Yunnan Province
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