抑肽酶对防治梨小食心虫的两种植物源农药的增效作用
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Citation：李俊杰,杨晓燕,唐慧琳,高俊恒,郭子坤,郭春阳,王冬寒,叶佳成,袁向群,李怡萍.抑肽酶对防治梨小食心虫的两种植物源农药的增效作用.Journal of Plant Protection,2025,52(1):105-112
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Author NameAffiliationE-mail
Li Junjie Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education
Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs
College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China 		 
Yang Xiaoyan Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education
Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs
College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China 		 
Tang Huilin Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education
Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs
College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China 		 
Gao Junheng Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education
Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs
College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China 		 
Guo Zikun Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education
Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs
College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China 		 
Guo Chunyang Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education
Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs
College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China 		 
Wang Donghan Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education
Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs
College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China 		 
Ye Jiacheng Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education
Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs
College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China 		 
Yuan Xiangqun Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education
Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs
College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China 		 
Li Yiping Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education
Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs
College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China 		liyiping@nwsuaf.edu.cn 
中文摘要:为提高植物源药剂对梨小食心虫Grapholita molesta的防治效果，测定饲喂含抑肽酶的人工饲料后梨小食心虫2龄幼虫中肠内总蛋白酶、胰蛋白酶和胰凝乳蛋白酶的活性，采用饲料混毒法测定0.20 mg/L抑肽酶对1%印楝素水分散粒剂和0.3%苦参碱水剂的增效作用。结果表明：饲喂含0.20 mg/L抑肽酶的人工饲料12 h后，梨小食心虫2龄幼虫中肠内总蛋白酶、胰蛋白酶和胰凝乳蛋白酶的活性均与对照无显著差异；饲喂36 h后，这3种蛋白酶的活性均较对照显著下降。当0.20 mg/L抑肽酶分别与LC10、LC30、LC50的1%印楝素水分散粒剂混配饲喂后，梨小食心虫2龄幼虫的实际死亡率分别为29.16%、43.06%和66.67%，均显著高于预测死亡率；当0.20 mg/L抑肽酶分别与LC10、LC30和LC50的0.3%苦参碱水剂混配饲喂后，梨小食心虫2龄幼虫的实际死亡率分别为44.44%、58.33%和68.89%，均显著高于预测死亡率。表明抑肽酶通过抑制梨小食心虫中肠蛋白酶活性对印楝素或苦参碱产生增效作用。
中文关键词:梨小食心虫  抑肽酶  苦参碱  印楝素  增效作用
 
Synergistic effect of aprotinin with two botanical pesticides against oriental fruit moth Grapholita molesta
Author NameAffiliationE-mail
Li Junjie Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education
Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs
College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China 		 
Yang Xiaoyan Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education
Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs
College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China 		 
Tang Huilin Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education
Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs
College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China 		 
Gao Junheng Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education
Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs
College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China 		 
Guo Zikun Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education
Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs
College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China 		 
Guo Chunyang Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education
Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs
College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China 		 
Wang Donghan Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education
Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs
College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China 		 
Ye Jiacheng Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education
Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs
College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China 		 
Yuan Xiangqun Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education
Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs
College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China 		 
Li Yiping Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education
Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs
College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China 		liyiping@nwsuaf.edu.cn 
Abstract:To improve the control effect of botanical pesticides on oriental fruit moth Grapholita molesta, the activities of total protease, trypsin and chymotrypsin in the midgut of the second larvae of G. molesta were measured after feeding an artificial diet containing 0.20 mg/L aprotinin. The synergistic effect of 0.20 mg/L aprotinin on 1% azadirachtin WG and 0.3% AS was determined by feeding on the diet containing these toxic substances. The results showed that after feeding the artificial diet containing aprotinin for 12 h, the activities of total protease, trypsin and chymotrypsin in the midgut of the second instar larvae were not significantly different from the control group. After feeding for 36 h, the activities of these three proteases decreased significantly compared with the control group. When 0.20 mg/L aprotinin was mixed with LC10, LC30 and LC50 of 1% azadirachtin WG, the actual mortality rates were 29.16%, 43.06% and 66.67%, respectively, which were significantly higher than the predicted mortality. When 0.20 mg/L aprotinin was mixed with LC10, LC30 and LC50 of 0.3% matrine AS, the actual mortality rates were 44.44%, 58.33% and 68.89%, respectively, which were significantly higher than the predicted mortality. These results indicate that aprotinin has a synergistic effect on azadirachtin or matrine by inhibiting the activity of the midgut proteases in G. molesta.
keywords:Grapholita molesta  aprotinin  matrine  azadirachtin  synergist effect
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