醛脱氢酶基因FvALDH-52和FvALDH-82对拟轮枝镰孢生长发育和致病力的影响 Click here to download the full text Citation：贾慧,任志国,张晓杰,刘宁,孙蔓莉,曹志艳,董金皋.醛脱氢酶基因FvALDH-52和FvALDH-82对拟轮枝镰孢生长发育和致病力的影响.Journal of Plant Protection,2025,52(1):201-210 Hits: Download times: Author Name Affiliation E-mail Jia Hui  Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, State Key Laboratory of North China Crop Improvement and Regulation, College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China    Ren Zhiguo  Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, State Key Laboratory of North China Crop Improvement and Regulation, College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China    Zhang Xiaojie  Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, State Key Laboratory of North China Crop Improvement and Regulation, College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China    Liu Ning  Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, State Key Laboratory of North China Crop Improvement and Regulation, College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China    Sun Manli  Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, State Key Laboratory of North China Crop Improvement and Regulation, College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China    Cao Zhiyan  Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, State Key Laboratory of North China Crop Improvement and Regulation, College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China  caozhiyan@hebau.edu.cn  Dong Jingao  Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, State Key Laboratory of North China Crop Improvement and Regulation, College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China  shmdjg@hebau.edu.cn  中文摘要:为明确醛脱氢酶（aldehyde dehydrogenase，ALDH）在玉米病原真菌拟轮枝镰孢Fusarium verticillioides生长发育及致病中的作用，利用实时荧光定量PCR（quantitative real-time polymerase chain reaction，qRT-PCR）技术分析拟轮枝镰孢的ALDH家族成员FvALDH-52和FvALDH-82基因在侵染玉米籽粒不同时期的表达水平，通过遗传转化方法对FvALDH-52和FvALDH-82进行基因敲除，分析2个基因在拟轮枝镰孢生长发育及致病性中的作用。结果表明：FvALDH-52和FvALDH-82在侵染玉米籽粒过程中表达水平均升高。敲除FvALDH-52和FvALDH-82基因后，拟轮枝镰孢的分生孢子产生能力降低，同时对H2O2和乙醛更加敏感，产生的伏马毒素显著降低；FvALDH-52基因敲除后导致病菌对玉米籽粒和玉米茎秆的致病力减弱。表明ALDH基因FvALDH-52在拟轮枝镰孢生长发育和致病过程中起着重要作用。 中文关键词:醛脱氢酶  拟轮枝镰孢  玉米病害  基因表达  功能分析   The aldehyde dehydrogenase genes FvALDH-52 and FvALDH-82 affect the growth, development and pathogenicity of fungal pathogen Fusarium verticillioides Author Name Affiliation E-mail Jia Hui  Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, State Key Laboratory of North China Crop Improvement and Regulation, College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China    Ren Zhiguo  Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, State Key Laboratory of North China Crop Improvement and Regulation, College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China    Zhang Xiaojie  Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, State Key Laboratory of North China Crop Improvement and Regulation, College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China    Liu Ning  Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, State Key Laboratory of North China Crop Improvement and Regulation, College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China    Sun Manli  Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, State Key Laboratory of North China Crop Improvement and Regulation, College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China    Cao Zhiyan  Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, State Key Laboratory of North China Crop Improvement and Regulation, College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China  caozhiyan@hebau.edu.cn  Dong Jingao  Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, State Key Laboratory of North China Crop Improvement and Regulation, College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China  shmdjg@hebau.edu.cn  Abstract:To clarify the function of aldehyde dehydrogenase (ALDH) in the growth, development, and pathogenicity of the maize pathogenic fungus Fusarium verticillioides, quantitative real-time polymerase chain reaction (qRT-PCR) was employed to analyze the expression levels of ALDH family members, the FvALDH-52 and FvALDH-82 genes of F. verticillioides, during different phases of its infection. Genetic transformation was carried out to knock out the FvALDH-52 and FvALDH-82 genes, with the aim of exploring the functions of these two genes in the growth, development, and pathogenicity of F. verticillioides. The results demonstrated that the expression levels of FvALDH-52 and FvALDH-82 increased during the infection process of maize kernels. Following the knockout of FvALDH-52 and FvALDH-82, the sporulation capacity of F. verticillioides decreased, and it became more sensitive to H2O2 and acetaldehyde, while the concentration of fumonitoxin declined substantially. The knockout of of FvALDH-52 weakened the pathogenicity of the pathogen towards kernels and stalks. These findings indicate that FvALDH-52 plays a crucial role in the growth, development, and pathogenicity of F. verticillioides. keywords:aldehyde dehydrogenase  Fusarium verticillioides  maize disease  gene expression  functional analysis View Full Text  View/Add Comment  Download reader

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