纳米二氧化硅增强蜡质芽胞杆菌AR156对水稻纹枯病的防效及其作用机制 Click here to download the full text Citation：姚志鹏,张紫谊,曹怡晗,王雨萌,牛冬冬.纳米二氧化硅增强蜡质芽胞杆菌AR156对水稻纹枯病的防效及其作用机制.Journal of Plant Protection,2025,52(2):351-365 Hits: Download times: Author Name Affiliation E-mail Yao Zhipeng  State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China    Zhang Ziyi  State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China    Cao yihan  State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China    Wang Yumeng  State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China    Niu Dongdong  State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China  ddniu@njau.edu.cn  中文摘要:为增强蜡质芽胞杆菌Bacillus cereus AR156的生防效果，将纳米二氧化硅（SiO2 nanoparticle，SiO2NP）与蜡质芽胞杆菌AR156结合，探究SiO2NP对蜡质芽胞杆菌AR156及水稻纹枯病病原菌立枯丝核菌Rhizoctonia solani的影响，并测定含SiO2NP的蜡质芽胞杆菌AR156菌悬液对水稻纹枯病的防效，分析其作用机制。结果显示：不同浓度SiO2NP对立枯丝核菌和蜡质芽胞杆菌AR156的生长无影响；浓度为500 μg/mL和1 000 μg/mL的SiO2NP能促进蜡质芽胞杆菌AR156生物膜的形成；含500 μg/mL和1 000 μg/mL SiO2NP的蜡质芽胞杆菌AR156菌悬液对水稻纹枯病的温室防效分别为68.39%和74.08%，比单独喷施蜡质芽胞杆菌AR156菌悬液的防效提高了10.43百分点和16.12百分点； SiO2NP不仅能提高蜡质芽胞杆菌AR156在水稻根围的定殖，而且能提高水稻体内过氧化氢酶、超氧化物歧化酶、过氧化物酶防卫相关酶活性及水稻病程相关基因非表达子（nonex‐pressor of pathogenesis-related gene 1，OsNPR1）和水稻病程相关蛋白1b （pathogenesis-related pro‐tein 1b，OsPR1b）基因的相对表达量。表明SiO2NP结合蜡质芽胞杆菌AR156在防治水稻纹枯病方面有较大的潜力。 中文关键词:水稻纹枯病  纳米二氧化硅  蜡质芽胞杆菌  抗病基因  防卫相关酶  温室防效   Efficacy enhancement and its mechanism of action of bacterium Bacillus cereus AR156 against rice sheath blight by silica nanoparticles Author Name Affiliation E-mail Yao Zhipeng  State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China    Zhang Ziyi  State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China    Cao yihan  State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China    Wang Yumeng  State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China    Niu Dongdong  State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China  ddniu@njau.edu.cn  Abstract:In order to enhance the biocontrol effect of Bacillus cereus AR156, SiO2 nanoparticle (SiO2NP) was combined with B. cereus AR156 to investigate the effect of SiO2NP on B. cereus AR156 and rice sheath blight fungus Rhizoctonia solani. While the effect of SiO2NP on B. cereus AR156 and R. solani was evaluated, the efficacy of SiO2NP-containing suspension of B. cereus AR156 on rice sheath blight was determined and its mechanism of action was analysed. The results showed that different concentrations of SiO 2NP had no detectable effect on the growth of R. solani and B. cereus AR156; the concentrations of 500 μg/mL and 1 000 μg/mL SiO2NP promoted the formation of biofilm of B. cereus AR156; and the effect of the suspension containing 500 μg/mL and 1 000 μg/mL SiO2NP on the control of rice blight in the greenhouse was determined with the analysis of the mechanism of action. The effect of above suspension on the control of rice sheath blight were in the glasshouse 68.39% and 74.08%, respectively, which were 10.43 and 16.12 percentage points higher than those of spraying B. cereu AR156 bacterial suspension alone; SiO2NP not only increased the colonization of B. cereus AR156 in the root perimeter of rice, but also increased the activities of the defence-related enzymes of peroxidase, superoxide dismutase and peroxidase, and the relative expression of nonexpressor of pathogenesisrelated gene 1 (OsNPR1) and pathogenesis-related protein 1b (OsPR1b) in rice. It indicates that SiO2NP combined with B. cereus AR156 has a great potential in controlling rice sheath blight. keywords:rice sheath blast  SiO2NP  Bacillus cereus  disease resistance gene  defence-related enzyme  greenhouse efficacy View Full Text  View/Add Comment  Download reader

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