桃红颈天牛雌雄成虫转录组差异分析 Click here to download the full text Citation：戚煜,刘欣玉,白妍妍,赵盈盈,谢寿安,王永平.桃红颈天牛雌雄成虫转录组差异分析.Journal of Plant Protection,2025,52(2):408-417 Hits: Download times: Author Name Affiliation E-mail Qi Yu  College of Forestry, Northwest A&F University, Yangling 712100, Shaanxi Province, China    Liu Xinyu  College of Forestry, Northwest A&F University, Yangling 712100, Shaanxi Province, China    Bai Yanyan  College of Forestry, Northwest A&F University, Yangling 712100, Shaanxi Province, China    Zhao Yingying  College of Forestry, Northwest A&F University, Yangling 712100, Shaanxi Province, China    Xie Shou?an  College of Forestry, Northwest A&F University, Yangling 712100, Shaanxi Province, China  shouanxie@163.com  Wang Yongping  Shaanxi Academy of Forestry, Xi' an 710003, Shaanxi Province, China    中文摘要:为明确桃红颈天牛Aromia bungii雌雄成虫转录组中的差异表达基因及其相关功能，采用高通量测序技术对雌雄成虫进行转录组测序，并对测序结果进行功能注释分析和差异表达基因的GO功能和KEGG通路富集分析。结果显示：从桃红颈天牛雌雄成虫转录组数据中共获得31 077个unigene，有21 613个unigene在NR、KOG、KEGG、GO、SwissProt、NT和PFAM七大数据库得到注释，其中在NR数据库获得注释的unigene最多，达18 201个。桃红颈天牛雌雄成虫间存在6 944个差异表达基因，其中雌成虫中相对于雄成虫显著上调基因有3 309个，显著下调基因有3 635个。基于差异表达基因的GO功能和KEGG通路富集分析，挖掘出8个可作为新型防治靶标的生殖相关基因，分别为卵黄原蛋白基因、卵黄原蛋白受体基因、类固醇急性调节蛋白基因、细胞周期蛋白B3基因、精液蛋白基因、精子发生相关蛋白6基因、透明质酸酶基因和cAMP依赖性蛋白激酶基因。 中文关键词:桃红颈天牛  转录组  高通量测序  差异表达基因  害虫防治  功能注释   Transcriptomic analysis of differential gene expression between female and male adults of red-necked longicorn Aromia bungii Author Name Affiliation E-mail Qi Yu  College of Forestry, Northwest A&F University, Yangling 712100, Shaanxi Province, China    Liu Xinyu  College of Forestry, Northwest A&F University, Yangling 712100, Shaanxi Province, China    Bai Yanyan  College of Forestry, Northwest A&F University, Yangling 712100, Shaanxi Province, China    Zhao Yingying  College of Forestry, Northwest A&F University, Yangling 712100, Shaanxi Province, China    Xie Shou?an  College of Forestry, Northwest A&F University, Yangling 712100, Shaanxi Province, China  shouanxie@163.com  Wang Yongping  Shaanxi Academy of Forestry, Xi' an 710003, Shaanxi Province, China    Abstract:To identify differentially expressed genes (DEGs) and their associated biological functions between male and female adults of red-necked longicorn Aromia bungii, high-throughput transcriptome sequencing was conducted. The sequencing data were subjected to functional annotation and enrichment analyses based on Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. A total of 31 077 unigenes were assembled, of which 21 613 were successfully annotated across seven major databases (NR, KOG, KEGG, GO, SwissProt, NT, and PFAM), with the NR database yielding the most annotations (18 201 unigenes). Comparative analysis revealed 6 944 DEGs between male and female adults, including 3 309 genes significantly up-regulated and 3 635 genes were significantly down-regulated in females relative to males. Functional enrichment analyses of these DEGs led to the identification of eight reproduction-related genes with potential as novel targets for pest control, including genes encoding vitellogenin, vitellogenin receptor, steroidogenic acute regulatory protein, cyclin-b3, seminal fluid protein, spermatogenesis-associated protein 6, hyaluronidase and cAMPdependent protein kinase. keywords:Aromia bungii  transcriptome  high-throughput sequencing  differentially expressed gene  pest management  functional annotation View Full Text  View/Add Comment  Download reader

Copyright:    京ICP备05006550号-2  You are the first  971344  Visitors
Head of Unit：Responsible Institution China Association for Scienc Organizers：China Society of Plant Protection and China Agricultural University Address：College of Agronomy and Biotechnology, China Agricultural University
Phone：86 (10) 62732528 E-mail：zbxb@cau.edu.cn
Technical Support：Tiller Beijing Science and Technology Development Co., Ltd.