| 广西五个稻区二化螟种群对氯虫苯甲酰胺的抗性及其遗传多样性分析 |
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| Citation：黄芊,龙丽萍,宁加和,陈伟源,吴碧球,李成,黄所生,黄凤宽,凌炎.广西五个稻区二化螟种群对氯虫苯甲酰胺的抗性及其遗传多样性分析.Journal of Plant Protection,2025,52(2):488-500 |
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| 中文摘要:为明确广西壮族自治区（简称广西）桂东、桂西、桂南、桂北和桂中5个稻区二化螟种群对氯虫苯甲酰胺的抗性水平及其遗传多样性，采用稻苗浸渍法测定这5个稻区二化螟种群对氯虫苯甲酰胺的抗性水平，以线粒体DNA （mitochondria DNA，mtDNA） CO II基因为分子标记，利用MEGA、DnaSP和Arlequin等软件对这5个稻区20个二化螟地理种群的遗传多样性、遗传分化、分子变异和种群动态进行分析。结果显示： 2023年广西13个二化螟地理种群对氯虫苯甲酰胺均为高水平抗性，抗性倍数介于113.7~513.5之间； 20个二化螟地理种群共检测到40种单倍型，36个变异位点，总群体的单倍型多样性为0.698，总群体间遗传分化指数和基因流分别为0.122和1.799； 5个稻区二化螟地理种群的遗传变异主要来自群体内个体间；桂南、桂北和桂中3个稻区二化螟地理种群很可能经历了种群扩张事件； 5个稻区二化螟地理种群的地理距离与遗传距离无显著相关性； 40个单倍型散布在不同的二化螟地理种群中，无明显的地理分布格局。表明广西5个稻区二化螟地理种群的遗传多样性较高，遗传分化程度低，基因交流水平较频繁，推测二化螟在面对环境变化时有较高的适应性和生存能力，这可能是二化螟在广西迅速扩散并对氯虫苯甲酰胺产生高水平抗性的主要原因之一。 |
| 中文关键词:二化螟 线粒体基因 地理种群 遗传分化 |
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| Analysis of chlorantraniliprole resistance and genetic diversity in Asiatic rice borer Chilo suppressalis populations from five rice-growing regions in Guangxi |
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| Abstract:To assess the resistance levels and genetic diversity of f Asiatic rice borer Chilo suppressalis populations across five rice-growing regions in Guangxi, China, the rice seedling dipping method was used to determine the resistance levels of populations from five regions. Mitochondrial CO II gene sequencing, analyzed using bioinformatics tools (MEGA, DnaSP, and Arlequin), was employed to analyze genetic diversity, genetic differentiation, molecular variation, and population dynamics across 20 geographical populations. The results showed that in 2023, all 13 tested populations exhibited high resistance to chlorantraniliprole, with resistance ratios ranging from 113.7 to 513.5. A total of 40 haplotypes and 36 polymorphic sites were identified among the 20 populations, with an overall haplotype diversity of 0.698. The genetic differentiation index was 0.122, and the gene flow was 1.799, indicating low genetic differentiation and frequent gene flow. Genetic variation in C. suppressalis populations from eastern, western, southern, northern and central regions of Guangxi primarily originated from withinpopulation diversity. Demographic analysis suggested that the southern, northern, and central populations likely experienced population expansion events. No significant correlation was found between geographical distance and genetic distance. The 40 haplotypes were scattered among the geographical populations without clear regional distribution patterns. These findings indicate that C. suppressalis populations in Guangxi exhibit high genetic diversity, low genetic differentiation, and frequent gene exchange, which likely contribute to their rapid regional spread and high-level resistance to chlorantraniliprole. This suggests that C. suppressalis has strong adaptability and survivability in response to environmental changes and insecticide pressure. |
| keywords:Chilo suppressalis mitochondrial gene geographical population genetic differentiation gene flow |
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