小麦条锈菌生理小种CYR34中GST基因家族的鉴定、结构特征及表达 Click here to download the full text Citation：周爽,韩宏,王春生,陈诗雯,杨成德,陶飞.小麦条锈菌生理小种CYR34中GST基因家族的鉴定、结构特征及表达.Journal of Plant Protection,2026,53(2):346-355 Hits: Download times: Author Name Affiliation E-mail Zhou Shuang  College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China    Han Hong  Academy of Agricultural Sciences of Linxia Hui Autonomous Prefecture, Linxia 731100, Gansu Province, China    Wang Chunsheng  College of Agriculture, Xinyang Agriculture and Forestry University, Xinyang 464006, Henan Province, China    Chen Shiwen  College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China    Yang Chengde  College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China    Tao Fei  College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China  taof@gsau.edu.cn  中文摘要:为阐明小麦条锈菌Puccinia striiformis f. sp. tritici生理小种CYR34的解毒代谢与环境适应机制，基于其基因序列文件及GFF3注释文件鉴定谷胱甘肽S-转移酶（glutathione S-transferase,GST）家族基因成员，对其进行蛋白理化性质、染色体定位、系统发育及启动子顺式作用元件等生物信息学分析，并通过实时荧光定量PCR技术分析GST基因家族在夏孢子萌发及侵染寄主过程中的时序表达模式。结果显示：在小麦条锈菌生理小种CYR34中共鉴定到6个GST基因（GST1～GST6），其编码蛋白长度介于187～207 aa之间，均含有GST家族相关保守结构域，并分布在3条染色体上。启动子区共检测到26类顺式作用元件。在夏孢子萌发过程中，GST1、GST3、GST4、GST5和GST6基因均在24 h达到较高水平，其中GST5基因上调幅度最大，为对照的9.04倍。在CYR34侵染小麦过程中，GST4和GST6基因的表达量整体呈上调趋势，其中GST4基因在侵染48 h时表达量最高，为对照的3.14倍。表明小麦条锈菌生理小种CYR34中GST基因可能参与夏孢子萌发及侵染过程中的环境适应与解毒代谢调控。 中文关键词:小麦条锈菌  生理小种  谷胱甘肽S-转移酶  基因家族  基因表达  夏孢子萌发  侵染   Identification, structural feature, and expression of the GST gene family in the physiological race CYR34 of Puccinia striiformis f. sp. tritici Author Name Affiliation E-mail Zhou Shuang  College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China    Han Hong  Academy of Agricultural Sciences of Linxia Hui Autonomous Prefecture, Linxia 731100, Gansu Province, China    Wang Chunsheng  College of Agriculture, Xinyang Agriculture and Forestry University, Xinyang 464006, Henan Province, China    Chen Shiwen  College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China    Yang Chengde  College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China    Tao Fei  College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China  taof@gsau.edu.cn  Abstract:To clarify the detoxification metabolism and environmental adaptation mechanisms of Puccinia striiformis f. sp. tritici(Pst) physiological race CYR34, members of the glutathione S-transferase(GST) gene family were identified based on the their nucleotide sequence and GFF3 annotation files of CYR34. Bioinformatics analyses were conducted on the physicochemical properties of the encoded proteins, chromosomal localization, phylogenetic relationships, and cis-acting elements in the promoter regions. In addition, the temporal expression patterns of GST family genes during urediniospore germination and host infection were analyzed by quantitative real-time PCR. The results showed that six GST genes(GST1-GST6) were identified in CYR34. The encoded proteins ranged from 187 to 207 amino acids in length, all containing conserved GST domains and being distributed on three chromosomes. A total of 26 types of cis-acting elements were detected in the promoter regions. During urediniospore germination, GST1, GST3, GST4, GST5, and GST6 all reached relatively high expression levels at 24 hours post inoculation(hpi), among which GST5 showed the greatest up-regulation, reaching 9.04-fold that of the control group. During wheat infection by CYR34, GST4 and GST6 generally exhibited an upward expression trend, with GST4 reaching its highest expression level at 48 hpi, 3.14-fold that of the control group. These results indicate that the GST genes in CYR34 may participate in the regulation of environmental adaptation and detoxification metabolism during urediniospore germination and infection. keywords:Puccinia striiformis f. sp. tritici  physiological race  glutathione S-transferase  gene family  gene expression  urediniospore germination  infection View Full Text  View/Add Comment  Download reader

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