番茄晚疫病菌拮抗菌株筛选鉴定及其作用机制 Click here to download the full text Citation：彭佳乐,杨平鸽,董欣杰,刘书剑,王磊,姚艳平,郝晓娟,王美琴.番茄晚疫病菌拮抗菌株筛选鉴定及其作用机制.Journal of Plant Protection,2026,53(2):356-366 Hits: Download times: Author Name Affiliation E-mail Peng Jiale  Shanxi Key Laboratory of Bioagent Utilization and Eco-Pesticide Innovation in Agriculture, College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Yang Pingge  Shanxi Key Laboratory of Bioagent Utilization and Eco-Pesticide Innovation in Agriculture, College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Dong Xinjie  Shanxi Key Laboratory of Bioagent Utilization and Eco-Pesticide Innovation in Agriculture, College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Liu Shujian  Shanxi Key Laboratory of Bioagent Utilization and Eco-Pesticide Innovation in Agriculture, College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Wang Lei  Shanxi Key Laboratory of Bioagent Utilization and Eco-Pesticide Innovation in Agriculture, College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Yao Yanping  Shanxi Key Laboratory of Bioagent Utilization and Eco-Pesticide Innovation in Agriculture, College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Hao Xiaojuan  Shanxi Key Laboratory of Bioagent Utilization and Eco-Pesticide Innovation in Agriculture, College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Wang Meiqin  Shanxi Key Laboratory of Bioagent Utilization and Eco-Pesticide Innovation in Agriculture, College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China  mqwang@sxau.edu.cn  中文摘要:为筛选防治由致病疫霉Phytophthora infestans引起的番茄晚疫病的菌种资源，采用平板对峙法从土壤中分离筛选出对致病疫霉拮抗作用较强的菌株，结合形态学、生理生化特征及16S rDNA基因序列分析其分类地位，通过菌丝生长、孢子萌发、盆栽试验等方法对其抑菌作用、抑菌机制和防治效果进行探究。结果表明：共筛选出11株菌株对致病疫霉具有拮抗作用，其中菌株C-21对致病疫霉的菌丝生长抑制率最高，为84.41%；菌株C-21最终被鉴定为链霉菌属Streptomyces的黄灰链霉菌S. flavogriseus；菌株C-21能产生纤维素酶、蛋白酶和淀粉酶等胞外酶；其发酵液能破坏致病疫霉的菌丝形态及细胞膜的完整性，显著抑制致病疫霉孢子囊萌发及释放游动孢子，抑制率分别为69.31%和57.94%；菌株C-21对番茄晚疫病的预防效果和治疗效果分别为43.57%和59.28%。表明菌株C-21对番茄晚疫病的防治效果较好，具有良好的开发应用潜力。 中文关键词:番茄晚疫病  致病疫霉  黄灰链霉菌  生物防治  作用机制   Isolation, identification of antagonistic bacterial strains, and the antagonistic mechanisms against tomato late blight pathogen Author Name Affiliation E-mail Peng Jiale  Shanxi Key Laboratory of Bioagent Utilization and Eco-Pesticide Innovation in Agriculture, College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Yang Pingge  Shanxi Key Laboratory of Bioagent Utilization and Eco-Pesticide Innovation in Agriculture, College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Dong Xinjie  Shanxi Key Laboratory of Bioagent Utilization and Eco-Pesticide Innovation in Agriculture, College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Liu Shujian  Shanxi Key Laboratory of Bioagent Utilization and Eco-Pesticide Innovation in Agriculture, College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Wang Lei  Shanxi Key Laboratory of Bioagent Utilization and Eco-Pesticide Innovation in Agriculture, College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Yao Yanping  Shanxi Key Laboratory of Bioagent Utilization and Eco-Pesticide Innovation in Agriculture, College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Hao Xiaojuan  Shanxi Key Laboratory of Bioagent Utilization and Eco-Pesticide Innovation in Agriculture, College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Wang Meiqin  Shanxi Key Laboratory of Bioagent Utilization and Eco-Pesticide Innovation in Agriculture, College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China  mqwang@sxau.edu.cn  Abstract:To screen microbial resources for the control of tomato late blight caused by pathogen Phytophthora infestans, strains with strong antagonistic activity were isolated from soil using the plate confrontation method. Their taxonomic status was determined using integrating morphological, physiological, and biochemical characteristics, combined with the 16S rDNA gene sequence analysis. The antifungal activity, action mechanisms, and control effect of the antagonistic strains were investigated via the mycelial growth inhibition method, spore germination assay, and pot experiment. The results showed that a total of 11 strains exhibited antagonistic activities against P. infestans. Among these antagonistic strains, strain C-21 exhibited the highest mycelial growth inhibition rate of 84.41%, and it was identified as Streptomyces flavogriseus. This strain could produce extracellular enzymes including cellulase, protease and amylase. Its fermentation broth could disrupt the mycelial morphology and cell membrane integrity of P. infestans, and significantly inhibited spore germination and zoospore release, with inhibition rates of 69.31% and 57.94%, respectively. The preventive and curative control effects of strain C-21 against tomato late blight were 43.57% and 59.28%, respectively. These findings indicated that strain C-21 had an excellent control effect on tomato late blight, and exhibited promising biocontrol potential for further development and application. keywords:tomato late blight  Phytophthora infestans  Streptomyces flavogriseus  biological control  action mechanism View Full Text  View/Add Comment  Download reader

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