宁夏酿酒葡萄根腐病病原鉴定及其拮抗菌株筛选 Click here to download the full text Citation：辛颖,赵楠,刘冠兰,蒲占悦,王一冬,顾沛雯.宁夏酿酒葡萄根腐病病原鉴定及其拮抗菌株筛选.Journal of Plant Protection,2026,53(2):367-382 Hits: Download times: Author Name Affiliation E-mail Xin Ying  College of Agriculture, Ningxia University, Yinchuan 750021, Ningxia Hui Autonomous Region, China Yinchuan Forest & Garden Pest Quarantine Inspection Station, Yinchuan 750021, Ningxia Hui Autonomous Region, China    Zhao Nan  College of Agriculture, Ningxia University, Yinchuan 750021, Ningxia Hui Autonomous Region, China    Liu Guanlan  College of Agriculture, Ningxia University, Yinchuan 750021, Ningxia Hui Autonomous Region, China    Pu Zhanyue  College of Agriculture, Ningxia University, Yinchuan 750021, Ningxia Hui Autonomous Region, China    Wang Yidong  College of Agriculture, Ningxia University, Yinchuan 750021, Ningxia Hui Autonomous Region, China    Gu Peiwen  College of Agriculture, Ningxia University, Yinchuan 750021, Ningxia Hui Autonomous Region, China  gupeiwen2019@nxu.edu.cn  中文摘要:为明确宁夏回族自治区（简称宁夏）酿酒葡萄根腐病的病原菌种类，挖掘当地土著拮抗微生物研发生防菌剂，从宁夏5个市县代表性酒庄葡萄园采集感根腐病葡萄样品，通过分离鉴定及回接试验明确病原菌种类，并以其中致病性较强的病原菌为指示菌，以酿酒葡萄品种赤霞珠为材料，采用平板对峙试验、菌丝生长抑制试验和盆栽试验检测从健康葡萄根际土壤中分离纯化的细菌和放线菌的生防效果。结果表明：宁夏酿酒葡萄根腐病病原菌有锐顶镰刀菌Fusarium acuminatum、腐皮镰刀菌Fusarium solani、立枯丝核菌Rhizoctonia solani、小新壳梭孢菌Neofusicoccum parvum和可可毛色二孢菌Lasiodiplodia theobromae，其中立枯丝核菌、小新壳梭孢菌和可可毛色二孢菌为葡萄根腐病病原菌的新记录种，且锐顶镰刀菌、立枯丝核菌、小新壳梭孢菌和可可毛色二孢菌的致病性较强。从健康葡萄根际土壤中共分离到62株菌落形态特征存在差异的菌株，筛选得到对上述4种致病性较强的病原菌均具有60%以上抑制率的4株细菌和放线菌菌株，经鉴定分别为贝莱斯芽胞杆菌Bacillus velezensis、苜蓿链霉菌Streptomyces alfalfae、东方拟无枝酸菌Amycolatopsis orientalis和灰色链霉菌Streptomyces griseus；其中贝莱斯芽胞杆菌的10.0%无菌培养液对4种供试病原菌的抑制作用最强，抑制率达到80.56%～84.65%，其挥发性物质对可可毛色二孢菌的抑制作用最强，抑制率达到73.67%；而3株拮抗放线菌菌株对不同病原菌的抑制作用有差异；采用伤根灌根法接种4株拮抗菌株均能不同程度抑制葡萄根腐病发生并促进根系分枝数量增加，说明筛选到的这4株拮抗菌株有良好的防病促生作用，具有开发为葡萄根腐病生防菌剂的潜力。 中文关键词:葡萄根腐病  病原鉴定  拮抗细菌  拮抗放线菌  抑制作用  生物防治  宁夏   Identification of grapevine root rot and screening of antagonistic bacterial strains in Ningxia wine grape growing region Author Name Affiliation E-mail Xin Ying  College of Agriculture, Ningxia University, Yinchuan 750021, Ningxia Hui Autonomous Region, China Yinchuan Forest & Garden Pest Quarantine Inspection Station, Yinchuan 750021, Ningxia Hui Autonomous Region, China    Zhao Nan  College of Agriculture, Ningxia University, Yinchuan 750021, Ningxia Hui Autonomous Region, China    Liu Guanlan  College of Agriculture, Ningxia University, Yinchuan 750021, Ningxia Hui Autonomous Region, China    Pu Zhanyue  College of Agriculture, Ningxia University, Yinchuan 750021, Ningxia Hui Autonomous Region, China    Wang Yidong  College of Agriculture, Ningxia University, Yinchuan 750021, Ningxia Hui Autonomous Region, China    Gu Peiwen  College of Agriculture, Ningxia University, Yinchuan 750021, Ningxia Hui Autonomous Region, China  gupeiwen2019@nxu.edu.cn  Abstract:To identify the pathogens causing grapevine root rot in Ningxia Hui Autonomous Region(hereinafter referred to as Ningxia) and to explore indigenous antagonistic microorganisms for developing biocontrol agents, grapevine samples with root rot symptoms were collected from representative winery vineyards in five cities and counties of Ningxia. The pathogens were identified through isolation, identification and pathogenicity tests. Using the more virulent pathogens as indicator strains and the wine grape cultivar Cabernet Sauvignon as the experimental material, the biocontrol efficacy of bacteria isolated from the rhizosphere soil of healthy grapevines was evaluated through plate confrontation assays, mycelial growth inhibition tests and pot experiments. The results showed that the pathogens causing grapevine root rot in Ningxia were Fusarium acuminatum, Fusarium solani, Rhizoctonia solani, Neofusicoccum parvum and Lasiodiplodia theobromae, among which R. solani, N. parvum and L. theobromae are new records as pathogens of grapevine root rot. Fusarium acuminatum, R. solani, N. parvum and L. theobromae exhibited stronger pathogenicity. A total of 62 bacterial and actinomycete strains with distinct colony morphologies were isolated from the rhizosphere soil of healthy grapevines, and four strains exhibiting over 60% inhibitory activity against all four highly pathogenic fungi were selected, namely Bacillus velezensis, Streptomyces alfalfae, Amycolatopsis orientalis and Streptomyces griseus. Among these, the 10.0% sterile culture filtrate of B. velezensis exhibited the strongest inhibitory effect against the four pathogens tested, with inhibition rates ranging from 80.56% to 84.65%, and its volatile compounds exhibited the strongest inhibitory effect against L. theobromae, with an inhibition rate of 73.67%. The three antagonistic actinomycetes showed differential inhibitory effects against different pathogens. Inoculation with the four antagonistic strains via wounded root irrigation inhibited grapevine root rot to varying degrees and promoted an increase in root branch number, indicating that these four antagonistic strains exhibit significant growth-promoting and disease-suppressing effects and possess potential for development as biocontrol agents against grape root rot. keywords:grapevine root rot  pathogen identification  antagonistic bacteria  antagonistic actinomycetes  inhibitory effect  biological control  Ningxia View Full Text  View/Add Comment  Download reader

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