内蒙古花生果腐病病原菌鉴定及其优势菌防治药剂的筛选 Click here to download the full text Citation：胡雪然,蒋超凡,何磊鸣,刘昊,程伟风,宋纪言,巩中军,李克斌,崔凯娣,周琳.内蒙古花生果腐病病原菌鉴定及其优势菌防治药剂的筛选.Journal of Plant Protection,2026,53(2):383-393 Hits: Download times: Author Name Affiliation E-mail Hu Xueran  Henan Research Center of Green Pesticide Engineering and Technology, Henan Key Laboratory of Creation and Application of New Pesticide, College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, Henan Province, China    Jiang Chaofan  Henan Research Center of Green Pesticide Engineering and Technology, Henan Key Laboratory of Creation and Application of New Pesticide, College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, Henan Province, China    He Leiming  Henan Research Center of Green Pesticide Engineering and Technology, Henan Key Laboratory of Creation and Application of New Pesticide, College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, Henan Province, China    Liu Hao  Henan Research Center of Green Pesticide Engineering and Technology, Henan Key Laboratory of Creation and Application of New Pesticide, College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, Henan Province, China    Cheng Weifeng  Henan Research Center of Green Pesticide Engineering and Technology, Henan Key Laboratory of Creation and Application of New Pesticide, College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, Henan Province, China    Song Jiyan  Henan Research Center of Green Pesticide Engineering and Technology, Henan Key Laboratory of Creation and Application of New Pesticide, College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, Henan Province, China    Gong Zhongjun  Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450046, Henan Province, China    Li Kebin  Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China    Cui Kaidi  Henan Research Center of Green Pesticide Engineering and Technology, Henan Key Laboratory of Creation and Application of New Pesticide, College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, Henan Province, China  ckd921209@163.com  Zhou Lin  Henan Research Center of Green Pesticide Engineering and Technology, Henan Key Laboratory of Creation and Application of New Pesticide, College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, Henan Province, China  zhoulinhenau@163.com  中文摘要:为有效防控花生果腐病，从内蒙古自治区通辽市清河镇采集具有典型花生果腐病的样品，采用单孢分离法对样品进行分离纯化获得菌株，对获得的菌株进行形态学观察与分子生物学鉴定，并测定琥珀酸脱氢酶抑制剂（succinate dehydrogenase inhibitor,SDHI）、甲氧基丙烯酸酯类（quinone outside inhibitor,QoI）和去甲基化抑制剂（demethylation inhibitor,DMI）3类10种杀菌剂对优势病原菌的室内毒力，筛选毒力较好的杀菌剂进行防效试验。结果显示：通过单孢分离法共获得11株菌株；经形态学观察和分子生物学鉴定，其中3株菌株为茄腐镰刀菌Fusarium solani,8株菌株为灰葡萄孢菌Botrytis cinerea，占比分别为27.27%和72.73%；灰葡萄孢菌为优势病原菌，对花生荚果和花生仁的平均病情指数分别为55.09和12.50。在SDHI、QoI和DMI类杀菌剂中，苯并烯氟菌唑、吡唑醚菌酯和氯氟醚菌唑对灰葡萄孢菌的抑制率最高。浓度100 mg/L的氯氟醚菌唑、吡唑醚菌酯和苯并烯氟菌唑对花生果腐病的保护防效和治疗防效分别为60.16%、76.42%、65.85%和58.54%、81.30%、68.29%，与对照药剂腐霉利的防效相当。表明氯氟醚菌唑、吡唑醚菌酯和苯并烯氟菌唑可用于防控花生果腐病。 中文关键词:花生果腐病  病原菌  灰葡萄孢菌  鉴定  杀菌剂  筛选  保护防效  治疗防效   Identification of pathogens causing peanut pod rot in Inner Mongolia and screening of control fungicides against the dominant pathogen Author Name Affiliation E-mail Hu Xueran  Henan Research Center of Green Pesticide Engineering and Technology, Henan Key Laboratory of Creation and Application of New Pesticide, College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, Henan Province, China    Jiang Chaofan  Henan Research Center of Green Pesticide Engineering and Technology, Henan Key Laboratory of Creation and Application of New Pesticide, College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, Henan Province, China    He Leiming  Henan Research Center of Green Pesticide Engineering and Technology, Henan Key Laboratory of Creation and Application of New Pesticide, College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, Henan Province, China    Liu Hao  Henan Research Center of Green Pesticide Engineering and Technology, Henan Key Laboratory of Creation and Application of New Pesticide, College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, Henan Province, China    Cheng Weifeng  Henan Research Center of Green Pesticide Engineering and Technology, Henan Key Laboratory of Creation and Application of New Pesticide, College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, Henan Province, China    Song Jiyan  Henan Research Center of Green Pesticide Engineering and Technology, Henan Key Laboratory of Creation and Application of New Pesticide, College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, Henan Province, China    Gong Zhongjun  Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450046, Henan Province, China    Li Kebin  Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China    Cui Kaidi  Henan Research Center of Green Pesticide Engineering and Technology, Henan Key Laboratory of Creation and Application of New Pesticide, College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, Henan Province, China  ckd921209@163.com  Zhou Lin  Henan Research Center of Green Pesticide Engineering and Technology, Henan Key Laboratory of Creation and Application of New Pesticide, College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, Henan Province, China  zhoulinhenau@163.com  Abstract:To effectively prevent and control peanut pod rot, samples showing typical symptoms were collected from Qinghe Town, Tongliao City, Inner Mongolia. The pathogens were isolated and purified using the single-spore isolation method. The obtained strains were identified with morphological and molecular biological techniques, and the toxicities of ten fungicides from succinate dehydrogenase inhibitor(SDHI), quinone outside inhibitor(QoI), and demethylation inhibitor(DMI) against dominant pathogens were determined, fungicides with high toxicity were further determined for their efficacy. A total of 11 fungal strains were obtained via single-spore isolation. Morphological and molecular identification revealed that three strains were identified as Fusarium solani and eight as Botrytis cinerea, accounting for 27.27% and 72.73%, respectively. Botrytis cinerea was the predominant pathogen(with average disease severity indices of 55.09 for peanut pods and 12.50 for peanut kernels). Among SDHI, QoI, and DMI fungicides, benzovindiflupyr, pyraclostrobin, and mefentrifluconazole exhibited the highest inhibition rates against B. cinerea. In vitro tissue experiments revealed that the protective efficacy of 100 mg/L mefentrifluconazole, pyraclostrobin, and benzovindiflupyr against peanut pod rot was 60.16%, 76.42%, and 65.85%, respectively; their corresponding curative efficacy was 58.54%, 81.30%, and 68.29%, respectively. These results were comparable to those of the reference fungicide procymidone. The results showed that mefentrifluconazole, pyraclostrobin, and benzovindiflupyr could be used to prevent and control peanut pod rot. keywords:peanut pod rot  pathogen  Botrytis cinerea  identification  fungicide  screening  protective efficacy  curative efficacy View Full Text  View/Add Comment  Download reader

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