| 甜菜碱对稻瘟病的防效及其诱导水稻抗瘟性的分子机制 |
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| Citation：李荣萍,唐敏,刘金汪,王云月,何平,韩光煜.甜菜碱对稻瘟病的防效及其诱导水稻抗瘟性的分子机制.Journal of Plant Protection,2026,53(2):394-408 |
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| Author Name | Affiliation | E-mail | | Li Rongping | State Key Laboratory for Conservation and Utilization of Bio-resources in Yunnan, Key Laboratory of the Ministry of Education for Agricultural Biodiversity and Pest Management, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, Yunnan Province, China | | | Tang Min | State Key Laboratory for Conservation and Utilization of Bio-resources in Yunnan, Key Laboratory of the Ministry of Education for Agricultural Biodiversity and Pest Management, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, Yunnan Province, China | | | Liu Jinwang | State Key Laboratory for Conservation and Utilization of Bio-resources in Yunnan, Key Laboratory of the Ministry of Education for Agricultural Biodiversity and Pest Management, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, Yunnan Province, China | | | Wang Yunyue | State Key Laboratory for Conservation and Utilization of Bio-resources in Yunnan, Key Laboratory of the Ministry of Education for Agricultural Biodiversity and Pest Management, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, Yunnan Province, China | | | He Ping | State Key Laboratory for Conservation and Utilization of Bio-resources in Yunnan, Key Laboratory of the Ministry of Education for Agricultural Biodiversity and Pest Management, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, Yunnan Province, China | pinghe@ynau.edu.cn | | Han Guangyu | State Key Laboratory for Conservation and Utilization of Bio-resources in Yunnan, Key Laboratory of the Ministry of Education for Agricultural Biodiversity and Pest Management, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, Yunnan Province, China | hanguangyu9745@163.com |
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| 中文摘要:为明确水稻代谢物甜菜碱对稻瘟病的防控效果及抗性诱导机制，通过外源喷施不同浓度甜菜碱以及不同时间点喷施甜菜碱鉴定水稻品种对稻瘟病的抗性，筛选甜菜碱外源喷施的最适浓度及最佳时间，并对最佳施用条件下喷施甜菜碱的水稻进行转录组测序，分析其抗性诱导机制。结果表明：外源施加甜菜碱可有效降低感病水稻品种黄壳糯、丽江新团黑谷的稻瘟病发生程度，且在接种稻瘟病菌Magnaporthe oryzae前24 h用20 mmol/L甜菜碱进行预处理对稻瘟病的防控效果最好，相对防效达40.06%。转录组分析结果表明，甜菜碱预处理后再接种稻瘟病菌0、24、48 h水稻叶片中的差异表达基因均在苯丙烷类生物合成通路显著富集，并诱导水稻木质素合成相关基因OsCCR17、OsCCR14、OsPAL3、OsCOMT15、OsCAD2及过氧化物酶基因OsPOX1的表达；木质素含量及过氧化物酶、超氧化物歧化酶的活性也显著升高。表明甜菜碱可以诱导水稻木质素合成基因及抗氧化酶基因的表达，促进木质素合成，提高抗氧化酶活性，从而提高水稻对稻瘟病的系统抗性。 |
| 中文关键词:稻瘟病 甜菜碱 转录组 木质素 抗氧化酶活性 |
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| Control effect of betaine against rice blast and its molecular mechanism of inducing rice blast resistance |
| Author Name | Affiliation | E-mail | | Li Rongping | State Key Laboratory for Conservation and Utilization of Bio-resources in Yunnan, Key Laboratory of the Ministry of Education for Agricultural Biodiversity and Pest Management, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, Yunnan Province, China | | | Tang Min | State Key Laboratory for Conservation and Utilization of Bio-resources in Yunnan, Key Laboratory of the Ministry of Education for Agricultural Biodiversity and Pest Management, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, Yunnan Province, China | | | Liu Jinwang | State Key Laboratory for Conservation and Utilization of Bio-resources in Yunnan, Key Laboratory of the Ministry of Education for Agricultural Biodiversity and Pest Management, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, Yunnan Province, China | | | Wang Yunyue | State Key Laboratory for Conservation and Utilization of Bio-resources in Yunnan, Key Laboratory of the Ministry of Education for Agricultural Biodiversity and Pest Management, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, Yunnan Province, China | | | He Ping | State Key Laboratory for Conservation and Utilization of Bio-resources in Yunnan, Key Laboratory of the Ministry of Education for Agricultural Biodiversity and Pest Management, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, Yunnan Province, China | pinghe@ynau.edu.cn | | Han Guangyu | State Key Laboratory for Conservation and Utilization of Bio-resources in Yunnan, Key Laboratory of the Ministry of Education for Agricultural Biodiversity and Pest Management, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, Yunnan Province, China | hanguangyu9745@163.com |
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| Abstract:To elucidate the control effect of the rice metabolite betaine on rice blast and its resistanceinducing mechanism, exogenous betaine sprays at different concentrations and time points were applied to assess the resistance of rice cultivars against rice blast. The optimal concentration and timing for exogenous betaine application were screened. Furthermore, transcriptome sequencing was performed on rice treated with betaine under the optimal application conditions to analyze its resistance induction mechanism. The results showed that exogenous application of betaine effectively reduced the severity of rice blast in the susceptible rice varieties Huangkenuo and Lijiangxintuanheigu. The optimal relative control effect(40.06%) was achieved with a pretreatment of 20 mmol/L betaine applied 24 hours before inoculation with Magnaporthe oryzae. Transcriptome analysis of rice leaves pretreated with betaine indicated that differentially expressed genes were significantly enriched in the phenylpropanoid biosynthesis pathway at 0, 24, and 48 hours. This treatment also induced the expression of lignin synthesisrelated genes, including OsCCR17, OsCCR14, OsPAL3, OsCOMT15, OsCAD2, and the peroxidase gene OsPOX1. Additionally, lignin content and the activities of peroxidase and superoxide dismutase were significantly increased. These findings suggest that betaine induces the expression of genes involved in lignin synthesis and antioxidant enzymes, promotes lignin accumulation, and enhances antioxidant enzyme activity, thereby improving systemic resistance in rice against rice blast. |
| keywords:rice blast betaine transcriptome lignin antioxidant enzyme activity |
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