沙阿霉素链霉菌XFS-4诱导大豆抗大豆胞囊线虫的转录组分析
Click here to download the full text
Citation：项鹏,李敏,杨树,王舒,李艳杰,李宝华,尤佳,张武.沙阿霉素链霉菌XFS-4诱导大豆抗大豆胞囊线虫的转录组分析.Journal of Plant Protection,2026,53(2):430-437
Hits:
Download times:
Author NameAffiliationE-mail
Xiang Peng Research Station of Field Harmful Organisms for Scientific Observation in Heihe, Heihe Branch of Heilongjiang Academy of Agricultural Sciences, Heihe 164300, Heilongjiang Province, China  
Li Min Research Station of Field Harmful Organisms for Scientific Observation in Heihe, Heihe Branch of Heilongjiang Academy of Agricultural Sciences, Heihe 164300, Heilongjiang Province, China  
Yang Shu Research Station of Field Harmful Organisms for Scientific Observation in Heihe, Heihe Branch of Heilongjiang Academy of Agricultural Sciences, Heihe 164300, Heilongjiang Province, China  
Wang Shu Research Station of Field Harmful Organisms for Scientific Observation in Heihe, Heihe Branch of Heilongjiang Academy of Agricultural Sciences, Heihe 164300, Heilongjiang Province, China  
Li Yanjie Research Station of Field Harmful Organisms for Scientific Observation in Heihe, Heihe Branch of Heilongjiang Academy of Agricultural Sciences, Heihe 164300, Heilongjiang Province, China  
Li Baohua Research Station of Field Harmful Organisms for Scientific Observation in Heihe, Heihe Branch of Heilongjiang Academy of Agricultural Sciences, Heihe 164300, Heilongjiang Province, China  
You Jia Soybean Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, Heilongjiang Province, China  
Zhang Wu Research Station of Field Harmful Organisms for Scientific Observation in Heihe, Heihe Branch of Heilongjiang Academy of Agricultural Sciences, Heihe 164300, Heilongjiang Province, China guoguo_zw@163.com 
中文摘要:为明确放线菌沙阿霉素链霉菌Streptomyces zaomyceticus XFS-4诱导大豆抗大豆胞囊线虫Heterodera glycines的分子机制，以大豆品种黑河43号为材料，经XFS-4发酵液拌种处理再接种大豆胞囊线虫后，采用RNA-seq测序技术解析其在转录水平的变化。结果显示：对4个处理组的大豆根部样本进行HiSeqTM 2500测序，获得78.66 Gb的数据，共得到1 633个差异表达基因。GO功能富集分析发现，沙阿霉素链霉菌XFS-4处理组的差异表达基因在含氮化合物代谢调控、大分子代谢调控和氧化还原酶活性功能上特异性富集；KEGG通路富集分析显示，沙阿霉素链霉菌XFS-4处理组和对照组的差异表达基因分别定位到95个和102个代谢途径分支，其中，阿霉素链霉菌处理组的差异表达基因在淀粉和蔗糖代谢通路、植物-病原物互作通路、辅酶因子生物合成通路显著富集。表明沙阿霉素链霉菌XFS-4可能通过影响这些代谢通路和生物过程来诱导大豆对大豆胞囊线虫产生抗性。
中文关键词:沙阿霉素链霉菌XFS-4  大豆胞囊线虫  转录组分析  差异表达基因  抗病机制
 
Transcriptome analysis of soybean resistance to soybean cyst nematode induced by bacterium Streptomyces zaomyceticus XFS-4
Author NameAffiliationE-mail
Xiang Peng Research Station of Field Harmful Organisms for Scientific Observation in Heihe, Heihe Branch of Heilongjiang Academy of Agricultural Sciences, Heihe 164300, Heilongjiang Province, China  
Li Min Research Station of Field Harmful Organisms for Scientific Observation in Heihe, Heihe Branch of Heilongjiang Academy of Agricultural Sciences, Heihe 164300, Heilongjiang Province, China  
Yang Shu Research Station of Field Harmful Organisms for Scientific Observation in Heihe, Heihe Branch of Heilongjiang Academy of Agricultural Sciences, Heihe 164300, Heilongjiang Province, China  
Wang Shu Research Station of Field Harmful Organisms for Scientific Observation in Heihe, Heihe Branch of Heilongjiang Academy of Agricultural Sciences, Heihe 164300, Heilongjiang Province, China  
Li Yanjie Research Station of Field Harmful Organisms for Scientific Observation in Heihe, Heihe Branch of Heilongjiang Academy of Agricultural Sciences, Heihe 164300, Heilongjiang Province, China  
Li Baohua Research Station of Field Harmful Organisms for Scientific Observation in Heihe, Heihe Branch of Heilongjiang Academy of Agricultural Sciences, Heihe 164300, Heilongjiang Province, China  
You Jia Soybean Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, Heilongjiang Province, China  
Zhang Wu Research Station of Field Harmful Organisms for Scientific Observation in Heihe, Heihe Branch of Heilongjiang Academy of Agricultural Sciences, Heihe 164300, Heilongjiang Province, China guoguo_zw@163.com 
Abstract:To elucidate the molecular mechanisms by which Streptomyces zaomyceticus XFS-4 induces soybean resistance to the soybean cyst nematode, Heterodera glycines, soybean variety Heihe 43 was used as the experimental material. Seeds were treated with XFS-4 fermentation broth and inoculated with H. glycines, and RNA-seq was performed to analyze transcriptional changes. The results showed that HiSeqTM 2500 sequencing of soybean root samples from four treatment groups yielded a total of 78.66 Gb of clean data, from which 1 633 differentially expressed genes（DEGs） were identified. GO enrichment analysis revealed that DEGs in the XFS-4 treatment group were specifically enriched in pathways related to the regulation of nitrogen compound metabolism, regulation of macromolecule metabolism, and oxidoreductase activity. KEGG pathway enrichment analysis showed that DEGs in the XFS-4 treatment group and the control group were mapped to 95 and 102 metabolic pathways, respectively. Notably, DEGs in the treatment group were significantly enriched in pathways related to starch and sucrose metabolism, plant-pathogen interaction, and biosynthesis of cofactors. These findings suggest that S. zaomyceticus XFS-4 may induce soybean resistance to H. glycines by regulating these specific metabolic pathways and biological processes.
keywords:Streptomyces zaomyceticus XFS-4  soybean cyst nematode  transcriptome analysis  differentially expressed genes  resistance mechanism
View Full Text  View/Add Comment  Download reader
Copyright:    京ICP备05006550号-2  You are the first  971344  Visitors
Head of Unit：Responsible Institution China Association for Scienc Organizers：China Society of Plant Protection and China Agricultural University Address：College of Agronomy and Biotechnology, China Agricultural University
Phone：86 (10) 62732528 E-mail：zbxb@cau.edu.cn
Technical Support：Tiller Beijing Science and Technology Development Co., Ltd.