基于全基因组重测序的宁波市二化螟种群遗传分析 Click here to download the full text Citation：肖山,柴伟纲,刘健,谌江华.基于全基因组重测序的宁波市二化螟种群遗传分析.Journal of Plant Protection,2026,53(2):449-458 Hits: Download times: Author Name Affiliation E-mail Xiao Shan  Ningbo Key Laboratory of Testing and Control for Characteristic Agro-Product Quality and Safety, Ningbo Academy of Agricultural Sciences, Ningbo 315040, Zhejiang Province, China    Chai Weigang  Ningbo Key Laboratory of Testing and Control for Characteristic Agro-Product Quality and Safety, Ningbo Academy of Agricultural Sciences, Ningbo 315040, Zhejiang Province, China    Liu Jian  Ningbo Key Laboratory of Testing and Control for Characteristic Agro-Product Quality and Safety, Ningbo Academy of Agricultural Sciences, Ningbo 315040, Zhejiang Province, China    Chen Jianghua  Ningbo Key Laboratory of Testing and Control for Characteristic Agro-Product Quality and Safety, Ningbo Academy of Agricultural Sciences, Ningbo 315040, Zhejiang Province, China  jhchen20@163.com  中文摘要:为解析宁波市二化螟Chilo suppressalis不同地理种群的遗传结构以及种群间亲缘关系，对8个二化螟种群（6个宁波市地理种群、1个北京种群和1个杭州种群）的37个样品进行全基因组重测序，鉴定基因组间遗传变异，并进行种群遗传结构、基因组选择性清除、遗传多样性、历史有效种群大小等种群遗传学分析。结果显示：在37个二化螟个体基因组中共鉴定到42 149 215个单核苷酸多态性和11 912 812个插入缺失，这些变异均主要分布在基因间和基因内含子区域。在宁波市6个二化螟种群中，鄞州、余姚、海曙种群的亲缘关系较近，象山种群与宁波市其他5个种群的亲缘关系最远，并表现出中度的遗传分化。二化螟象山种群基因组选择性清除区域内的基因显著富集在黑色素生成、昼夜节律夹带等7个信号通路。在宁波市各种群中，海曙种群和鄞州种群的核苷酸多样性较高，象山种群和奉化种群的核苷酸多样性较低。宁波市二化螟种群在3 000年前—1 000年前左右经历了快速收缩，随后种群规模快速增加。 中文关键词:二化螟  全基因组重测序  种群遗传结构  遗传多样性  遗传变异  遗传分化   Genetic analysis of Asiatic rice borer Chilo suppressalis populations in Ningbo City based on whole-genome resequencing Author Name Affiliation E-mail Xiao Shan  Ningbo Key Laboratory of Testing and Control for Characteristic Agro-Product Quality and Safety, Ningbo Academy of Agricultural Sciences, Ningbo 315040, Zhejiang Province, China    Chai Weigang  Ningbo Key Laboratory of Testing and Control for Characteristic Agro-Product Quality and Safety, Ningbo Academy of Agricultural Sciences, Ningbo 315040, Zhejiang Province, China    Liu Jian  Ningbo Key Laboratory of Testing and Control for Characteristic Agro-Product Quality and Safety, Ningbo Academy of Agricultural Sciences, Ningbo 315040, Zhejiang Province, China    Chen Jianghua  Ningbo Key Laboratory of Testing and Control for Characteristic Agro-Product Quality and Safety, Ningbo Academy of Agricultural Sciences, Ningbo 315040, Zhejiang Province, China  jhchen20@163.com  Abstract:To investigate the population structure and phylogenetic relationships among different geographic populations of Asiatic rice borer Chilo suppressalis in Ningbo City, China,whole-genome resequencing was performed on 37 individuals from eight populations(six from Ningbo, one from Beijing, and one from Hangzhou). Genomic variations were identified, and population genetic analyses including population structure, selective sweeps, genetic diversity, and historical effective population size were conducted. The results showed that a total of 42 149 215 single nucleotide polymorphisms and 11 912 812 insertions/deletions were identified across the 37 genomes, with most variations located in intergenic and intronic regions. Among the six Ningbo populations, Yinzhou, Yuyao and Haishu populations were closely related, whereas the Xiangshan population showed the greatest genetic distance from the other Ningbo populations and exhibited moderate genetic differentiation. Genes within the selective sweep regions of the Xiangshan population was significantly enriched in seven signaling pathways, including melanogenesis and circadian entrainment. Among the Ningbo populations, nucleotide diversity was relatively higher in the Haishu and Yinzhou populations, but lower in the Xiangshan and Fenghua populations. The Ningbo populations experienced a rapid contraction approximately 3 000—1 000 years ago, followed by a rapid population expansion. keywords:Chilo suppressalis  whole genome resequencing  population genetic structure  genetic diversity  genetic variation  genetic differentiation View Full Text  View/Add Comment  Download reader

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