细小果胶杆菌在黑腹果蝇体内的定殖能力和适应性分析 Click here to download the full text Citation：安帅霖,姚亚林,杨炳樟,王金辉,董金皋.细小果胶杆菌在黑腹果蝇体内的定殖能力和适应性分析.Journal of Plant Protection,2026,53(2):477-486 Hits: Download times: Author Name Affiliation E-mail An Shuailin  College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China    Yao Yalin  College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China    Yang Bingzhang  College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China    Wang Jinhui  College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China  jinhwang@hebau.edu.cn  Dong Jingao  College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China  dongjingao@126.com  中文摘要:为明确细小果胶杆菌Pectobacterium parvum对媒介昆虫黑腹果蝇Drosophila melanogaster的适应性，通过人工饲喂方式对黑腹果蝇回补细小果胶杆菌，结合实时荧光定量PCR技术比较细小果胶杆菌与其近缘种在黑腹果蝇体内的定殖存活能力。结果显示：人工饲喂黑腹果蝇含菌葡萄糖液后7 d内，细小果胶杆菌在黑腹果蝇体内的定殖能力与巴西果胶杆菌P. brasiliense相当，相对含菌量达到8.00，明显高于帕曼氏果胶杆菌P. parmentieri、极地果胶杆菌P. polaris和波兰果胶杆菌P. polonicum的定殖能力；且细小果胶杆菌可经黑腹果蝇卵实现高效垂直传播，其传播效率显著高于其他近缘种，在第2代幼虫体内的相对含菌量达8.44，远高于其余果胶杆菌的相对含菌量；此外，细小果胶杆菌可显著抑制黑腹果蝇免疫缺陷Imd通路关键基因PGRP-LE和Relish的表达，而极地果胶杆菌则显著诱导了这些基因的表达。表明细小果胶杆菌对黑腹果蝇宿主的适应性显著强于近缘种，这与其较强的体内定殖能力与高效的经卵垂直传播能力以及对宿主Imd免疫通路关键基因的显著抑制作用密切相关。 中文关键词:果胶杆菌  细小果胶杆菌  黑腹果蝇  昆虫媒介  垂直传播   Colonization capacity and adaptability of bacterial pathogen Pectobacterium parvum in common fruit fly Drosophila melanogaster Author Name Affiliation E-mail An Shuailin  College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China    Yao Yalin  College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China    Yang Bingzhang  College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China    Wang Jinhui  College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China  jinhwang@hebau.edu.cn  Dong Jingao  College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China  dongjingao@126.com  Abstract:To clarify the adaptability of bacterial pathogen Pectobacterium parvum to its vector insect Drosophila melanogaster, flies were recolonized with P. parvum via artificial feeding, and the colonization and survival capabilities of P. parvum and its closely related species in D. melanogaster were compared using quantitative real-time PCR. The results showed that within seven days after feeding D. melanogaster with bacteria-containing glucose solution, the colonization capability of P. parvum in D. melanogaster was comparable to that of P. brasiliense, with a relative bacterial load reaching 8.00, and evidently higher than those of P. parmentieri, P. polaris and P. polonicum. Additionally, P. parvum could achieve efficient vertical transmission via D. melanogaster eggs, with a transmission efficiency significantly higher than that of other closely related species. The relative bacterial load in secondgeneration larvae reached 8.44, far higher than that of other Pectobacterium species. Furthermore, P. parvum significantly suppressed the expression of key genes in the host Imd immune pathway, including PGRP-LE and Relish, whereas P. polaris significantly induced the expression of these immune genes. These results indicate that P. parvum exhibits significantly stronger adaptation to D. melanogaster than its closely related species, which is closely associated with its strong in vivo colonization capacity, efficient egg-mediated vertical transmission, and significant suppression of key genes in the host Imd immune pathway. keywords:Pectobacterium  Pectobacterium parvum  Drosophila melanogaster  insect vector  vertical transmission View Full Text  View/Add Comment  Download reader

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