转Cry基因抗虫水稻和Cry蛋白对褐飞虱的杀虫活性 Click here to download the full text Citation：王渭霞,赖凤香,何佳春,魏琪,万品俊,傅强.转Cry基因抗虫水稻和Cry蛋白对褐飞虱的杀虫活性.Journal of Plant Protection,2026,53(2):487-497 Hits: Download times: Author Name Affiliation E-mail Wang Weixia  Ministry of Agriculture and Rural Affairs Center for Plant Environmental Safety Assessment (Hangzhou), China National Rice Research Institute, Hangzhou 311401, Zhejiang Province, China  wangweixia@caas.cn  Lai Fengxiang  Ministry of Agriculture and Rural Affairs Center for Plant Environmental Safety Assessment (Hangzhou), China National Rice Research Institute, Hangzhou 311401, Zhejiang Province, China    He Jiachun  Ministry of Agriculture and Rural Affairs Center for Plant Environmental Safety Assessment (Hangzhou), China National Rice Research Institute, Hangzhou 311401, Zhejiang Province, China    Wei Qi  Ministry of Agriculture and Rural Affairs Center for Plant Environmental Safety Assessment (Hangzhou), China National Rice Research Institute, Hangzhou 311401, Zhejiang Province, China    Wan Pinjun  Ministry of Agriculture and Rural Affairs Center for Plant Environmental Safety Assessment (Hangzhou), China National Rice Research Institute, Hangzhou 311401, Zhejiang Province, China    Fu Qiang  Ministry of Agriculture and Rural Affairs Center for Plant Environmental Safety Assessment (Hangzhou), China National Rice Research Institute, Hangzhou 311401, Zhejiang Province, China    中文摘要:为明确转Cry基因水稻与褐飞虱Nilaparvata lugens抗性之间的关系，通过饲喂添加Cry蛋白的人工饲料筛选出对褐飞虱有杀虫活性的蛋白，并测定该蛋白对褐飞虱的毒力；利用含相应杀虫蛋白的转基因水稻饲喂褐飞虱验证其杀虫活性；利用酶联免疫吸附试验测定不同转Cry基因水稻茎秆中杀虫蛋白浓度和添加不同杀虫蛋白的饲料中杀虫蛋白浓度以及取食上述茎秆和饲料后褐飞虱体内杀虫蛋白的浓度。结果显示：取食含Cry2A、Cry1Ah、Cry1F和Cry1Ba蛋白的人工饲料显著降低了褐飞虱的存活率。Cry1Ah、Cry1F和Cry1Ba蛋白对褐飞虱的LC50分别为95.41、13.89和559.7 mg/L。取食转基因水稻KF6（含Cry1Ac和CpTi基因）、TT51-1（含Cry1Ac/Cry1Ab融合基因）和ZLSAH10（含Cry1Ah基因）对褐飞虱存活率无显著影响，但取食转基因水稻TT51-1的褐飞虱雌成虫体重较对照显著下降。取食含100 mg/L Cry蛋白的人工饲料1 d后，褐飞虱体内就能检测到Cry蛋白，且体内积累量随取食浓度和取食时间的增加而增加。转基因水稻TT51-1和ZLSAH10茎秆中Cry蛋白浓度在3个生育期无显著差异，而转基因水稻KF6茎秆中Cry浓度在3个生育期差异显著。取食不同生育期的转Cry基因水稻15 d后，褐飞虱体内均能检测到Cry蛋白，约为转Cry基因水稻茎秆中的0.1%，但褐飞虱体内Cry蛋白浓度的变化趋势与各生育期转Cry基因水稻内Cry蛋白浓度的变化趋势并不一致。 中文关键词:转基因水稻  Cry蛋白  褐飞虱  人工饲料  毒力   Insecticidal activity of transgenic Cry rice and Cry proteins against the brown planthopper Nilaparvata lugens Author Name Affiliation E-mail Wang Weixia  Ministry of Agriculture and Rural Affairs Center for Plant Environmental Safety Assessment (Hangzhou), China National Rice Research Institute, Hangzhou 311401, Zhejiang Province, China  wangweixia@caas.cn  Lai Fengxiang  Ministry of Agriculture and Rural Affairs Center for Plant Environmental Safety Assessment (Hangzhou), China National Rice Research Institute, Hangzhou 311401, Zhejiang Province, China    He Jiachun  Ministry of Agriculture and Rural Affairs Center for Plant Environmental Safety Assessment (Hangzhou), China National Rice Research Institute, Hangzhou 311401, Zhejiang Province, China    Wei Qi  Ministry of Agriculture and Rural Affairs Center for Plant Environmental Safety Assessment (Hangzhou), China National Rice Research Institute, Hangzhou 311401, Zhejiang Province, China    Wan Pinjun  Ministry of Agriculture and Rural Affairs Center for Plant Environmental Safety Assessment (Hangzhou), China National Rice Research Institute, Hangzhou 311401, Zhejiang Province, China    Fu Qiang  Ministry of Agriculture and Rural Affairs Center for Plant Environmental Safety Assessment (Hangzhou), China National Rice Research Institute, Hangzhou 311401, Zhejiang Province, China    Abstract:To clarify the relationship between transgenic Cry rice and resistance to the brown planthopper Nilaparvata lugens, proteins with insecticidal activity against N. lugens were screened using an artificial diet supplemented with proteins, and their toxicity was determined. Transgenic rice containing the corresponding insecticidal proteins was then used to feed N. lugens to verify its insecticidal activity. Enzyme-linked immunosorbent assay（ELISA） was employed to measure the concentrations of Cry proteins in stems of transgenic rice, in diets supplemented with different Cry proteins, and in N. lugens after feeding on these stems and diets. The results showed that feeding on artificial diets containing Cry2A, Cry1 Ah, Cry1F, and Cry1 Ba significantly reduced the survival rate of N. lugens. The LC50 values of Cry1 Ah, Cry1F, and Cry1 Ba against N. lugens were 95.41, 13.89, and 559.7 mg/L, respectively. Feeding on transgenic rice KF6（containing Cry1 Ac and CpTi genes）, TT51-1（containing Cry1 Ac/Cry1 Ab genes）, and ZLSAH10（containing Cry1 Ah gene） had no significant effect on survival rate, but the body weight of female adults fed on TT51-1 was significantly reduced compared to the control. Cry proteins were be detectable in N. lugens after one day of feeding on an artificial diet containing 100 mg/L Cry protein, and their accumulation increased with increasing feeding concentration and duration. The Cry protein concentrations in stems of transgenic rice TT51-1 and ZLSAH10 did not differ significantly among the three growth stages, while those in KF6 differed significantly among stages. After feeding on transgenic Cry rice at different growth periods for 15 d, Cry proteins were detectable in N. lugens, accounting for approximately 0.1% of those in rice stems, but the variation trend of Cry protein concentration in N. lugens was not consistent with that in rice stems. keywords:transgenic rice  Cry protein  brown planthopper  artificial diet  toxicity View Full Text  View/Add Comment  Download reader

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