靶向FPPS基因的RNA干扰技术对农业害螨的防控效果评估 Click here to download the full text Citation：李刚,李庆艳,李卓,曹璋光,于洪文,李忠意,古欣瑶.靶向FPPS基因的RNA干扰技术对农业害螨的防控效果评估.Journal of Plant Protection,2026,53(2):498-506 Hits: Download times: Author Name Affiliation E-mail Li Gang  Institute of Entomology, Guizhou University, Guiyang 550025, Guizhou Province, China    Li Qingyan  Institute of Entomology, Guizhou University, Guiyang 550025, Guizhou Province, China    Li Zhuo  Institute of Entomology, Guizhou University, Guiyang 550025, Guizhou Province, China    Cao Zhangguang  Institute of Entomology, Guizhou University, Guiyang 550025, Guizhou Province, China    Yu Hongwen  Institute of Entomology, Guizhou University, Guiyang 550025, Guizhou Province, China    Li Zhongyi  College of Animal Science, Guizhou University, Guiyang 550025, Guizhou Province, China    Gu Xinyao  College of Animal Science, Guizhou University, Guiyang 550025, Guizhou Province, China  guxy@gzu.edu.cn  中文摘要:为明确保幼激素合成关键酶——法尼基焦磷酸合成酶（farnesyl pyrophosphate synthase,FPPS）作为害螨RNA干扰防控靶标的可行性，通过dsRNAEngineer在线平台设计FPPS的dsRNA(ds FPPS)，采用注射法测定其对农业重要害螨二斑叶螨Tetranychus urticae和伊氏叶螨Tetranychus evansi的生物活性，采用实时荧光定量PCR技术检测注射ds FPPS后害螨体内FPPS的表达水平，并通过饲喂法和注射法分别评价其对非靶标生物加州新小绥螨Neoseiulus californicus和异色瓢虫Harmonia axyridis的安全性。结果显示：注射ds FPPS后，二斑叶螨和伊氏叶螨体内FPPS的表达量分别显著降低了91.80%和83.09%；两种叶螨后若螨均无法正常蜕皮而死亡，死亡率分别为76.97%和84.32%。饲喂或注射ds FPPS后加州新小绥螨和异色瓢虫均发育正常，证明其对天敌生物无显著影响。表明ds FPPS对害螨具有高效致死性且对非靶标生物安全，FPPS基因可作为叶螨RNA干扰防控的理想靶标，具有开发为新型绿色杀螨剂的潜力。 中文关键词:叶螨  RNA干扰  法尼基焦磷酸合成酶  特异性dsRNA  生物安全  防控靶标   Evaluation of the control efficacy of RNA interference targeting the FPPS gene against pest mites Author Name Affiliation E-mail Li Gang  Institute of Entomology, Guizhou University, Guiyang 550025, Guizhou Province, China    Li Qingyan  Institute of Entomology, Guizhou University, Guiyang 550025, Guizhou Province, China    Li Zhuo  Institute of Entomology, Guizhou University, Guiyang 550025, Guizhou Province, China    Cao Zhangguang  Institute of Entomology, Guizhou University, Guiyang 550025, Guizhou Province, China    Yu Hongwen  Institute of Entomology, Guizhou University, Guiyang 550025, Guizhou Province, China    Li Zhongyi  College of Animal Science, Guizhou University, Guiyang 550025, Guizhou Province, China    Gu Xinyao  College of Animal Science, Guizhou University, Guiyang 550025, Guizhou Province, China  guxy@gzu.edu.cn  Abstract:To assess the feasibility of farnesyl pyrophosphate synthase(FPPS), a key enzyme in juvenile hormone biosynthesis, as a target for RNA interference(RNAi)-based control of pest mites, doublestranded RNA targeting FPPS(ds FPPS) was designed using the dsRNAEngineer online platform. The bioactivity of ds FPPS against two agriculturally important pest mites, Tetranychus urticae and Tetranychus evansi, was evaluated with microinjection. The expression levels of FPPS in mites after ds FPPS injection were determined by quantitative real-time PCR, and the safety to non-target organisms, Neoseiulus californicus and Harmonia axyridis, was assessed by feeding and microinjection assays. The results showed that after ds FPPS injection, FPPS transcript levels in T. urticae and T. evansi were significantly reduced by 91.80% and 83.09%, respectively. Deutonymphs of both mite species failed to molt normally and died, with mortality rates of 76.97% and 84.32%, respectively. After feeding on or microinjection ds FPPS, N. californicus and H. axyridis developed normally, indicating no significant effects on these natural enemies. These findings demonstrate that ds FPPS has high lethality against pest mites while being safe for non-target organisms. The FPPS gene can therefore serve as an ideal target for RNAi-based control of pest mites and has potential for development as a novel environmentally friendly acaricide. keywords:spider mites  RNA interference  farnesyl pyrophosphate synthetase  specific dsRNA  bio-safety  control target View Full Text  View/Add Comment  Download reader

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