茶园三刺角蝉自然流行白僵病病原菌鉴定、种群遗传多样性及其致病力分析 Click here to download the full text Citation：王定锋,李慧玲,李良德,李金玉,杨军,张辉.茶园三刺角蝉自然流行白僵病病原菌鉴定、种群遗传多样性及其致病力分析.Journal of Plant Protection,2026,53(2):507-518 Hits: Download times: Author Name Affiliation E-mail Wang Dingfeng  Fujian Branch of the National Tea Improvement Center, Tea Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, Fujian Province, China  wangdf2022@163.com  Li Huiling  Fujian Branch of the National Tea Improvement Center, Tea Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, Fujian Province, China    Li Liangde  Fujian Branch of the National Tea Improvement Center, Tea Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, Fujian Province, China    Li Jinyu  Fujian Branch of the National Tea Improvement Center, Tea Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, Fujian Province, China    Yang Jun  Fujian Branch of the National Tea Improvement Center, Tea Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, Fujian Province, China    Zhang Hui  Fujian Branch of the National Tea Improvement Center, Tea Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, Fujian Province, China    中文摘要:为明确茶园三刺角蝉Tricentrus spp.自然流行白僵病病原菌及其对茶小绿叶蝉Empoasca onukii的防治潜力，对该茶园中采集的不同僵虫样本进行分离、纯化及培养，基于形态学特征与基于Bloc、RPB1、RPB2和TEF基因序列联合构建的系统发育树对其分类地位进行综合分析；利用12对SSR引物分析不同寄主来源病原菌种群的遗传多样性；选取寄主为角蝉科和叶蝉科的6株菌株，测定其对茶小绿叶蝉3龄若虫的毒力。结果表明：该茶园三刺角蝉自然流行白僵病的病原菌均为球孢白僵菌Beauveria bassiana。球孢白僵菌群体遗传多样性水平较高，多态位点百分率为100.00%,Shannon信息指数为0.73,Nei’s基因多样性指数为0.39。不同寄主来源的4个球孢白僵菌种群之间的遗传分化系数为0.38，且基因流为0.54，表明种群间基因交流不畅。89株菌株可分为31个基因型，当相似系数为0.68时，这89株菌株被聚为3个分支，其中分支Ⅲ包含76.40%的菌株，为优势类群。菌株JCPX121的毒力最强，经浓度为1.0×108个/mL的孢子悬浮液处理8 d后，茶小绿叶蝉3龄若虫的校正死亡率为80.66%，致死中时为5.90 d。综上，菌株JCPX121具有开发为生防菌剂的潜力。 中文关键词:三刺角蝉  白僵病  球孢白僵菌  自然流行病  遗传多样性  毒力   Identification of the pathogen causing a natural epizootic of white muscardine in treehopper Tricentrus sp. in a tea plantation, and analyses of population genetic diversity and its pathogenicity Author Name Affiliation E-mail Wang Dingfeng  Fujian Branch of the National Tea Improvement Center, Tea Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, Fujian Province, China  wangdf2022@163.com  Li Huiling  Fujian Branch of the National Tea Improvement Center, Tea Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, Fujian Province, China    Li Liangde  Fujian Branch of the National Tea Improvement Center, Tea Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, Fujian Province, China    Li Jinyu  Fujian Branch of the National Tea Improvement Center, Tea Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, Fujian Province, China    Yang Jun  Fujian Branch of the National Tea Improvement Center, Tea Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, Fujian Province, China    Zhang Hui  Fujian Branch of the National Tea Improvement Center, Tea Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, Fujian Province, China    Abstract:To identify the pathogen causing a natural epizootic of white muscardine in Tricentrus sp. in a tea plantation and to evaluate its potential for controlling tea green leafhopper Empoasca onukii, cadaver samples collected from the tea plantation were subjected to isolation, purification, and culture. The taxonomic status of the pathogen was determined based on morphological characteristics and phylogenetic analysis using combined sequences of Bloc, RPB1, RPB2, and TEF genes. Population genetic diversity of isolates from different hosts was analyzed using 12 SSR primer pairs. Six strains derived from hosts in Membracidea and Cicadellidae were selected to evaluate their virulence against the 3 rd instar nymphs of E. onukii. The results showed that the pathogen causing the natural epizootic of white muscardine in Tricentrus sp. in the tea plantation was B. bassiana. The B. bassiana populations exhibited a high level of genetic diversity, with 100.00% polymorphic loci, a Shannon's information index of 0.73, and a Nei's gene diversity index of 0.39. Among the four B. bassiana populations from different hosts, the genetic differentiation coefficient was 0.38 and the gene flow was 0.54, indicating limited genetic exchange among populations. A total of 89 isolates were divided into 31 genotypes and clustered into three groups at a similarity coefficient of 0.68, with group Ⅲ accounting for 76.40% of the isolates and representing the dominant group. Strain JCPX121 exhibited the highest virulence. After treatment with a conidial suspension at a concentration of 1.0×108 conidia/mL for eight days, the corrected mortality rate of 3 rd instar nymphs of E. onukii reached 80.66% and the median lethal time(LT50) was 5.90 d. In summary, strain JCPX121 has the potential to be developed as a biocontrol agent. keywords:Tricentrus sp.  white muscardine  Beauveria bassiana  natural epizootic  genetic diversity  virulence View Full Text  View/Add Comment  Download reader

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