植物挥发物的筛选及其与硅藻土联合对绿豆象防效和酶活性的影响 Click here to download the full text Citation：李子鹏,李慧倩,苑潇丹,武安琪,张仙红,张泽燕,郑海霞.植物挥发物的筛选及其与硅藻土联合对绿豆象防效和酶活性的影响.Journal of Plant Protection,2026,53(2):527-536 Hits: Download times: Author Name Affiliation E-mail Li Zipeng  College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Li Huiqian  College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Yuan Xiaodan  College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Wu Anqi  College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Zhang Xianhong  College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Zhang Zeyan  College of Agriculture, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China  447401080@qq.com  Zheng Haixia  College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China  zhenghaixia722@163.com  中文摘要:为寻求绿色高效防治绿豆象Callosobruchus chinensis的方法，采用密闭熏蒸法测定茴香烯、柠檬醛、4-烯丙基苯甲醚、丁香酚4种植物挥发物对绿豆象成虫的熏蒸效果，选择熏蒸效果最佳的植物挥发物，将其与硅藻土联合，测定联合后对绿豆象成虫的室内毒力、防效及其对绿豆象体内多种酶活性的影响。结果显示：在4种植物挥发物中茴香烯对绿豆象成虫的熏蒸效果最佳，5μL/L茴香烯处理48 h时，绿豆象成虫的校正死亡率达到100.00%；茴香烯和硅藻土联合处理表现出显著的协同增效作用，其对绿豆象成虫的半致死剂量LC50为3.040μL/L，而茴香烯对绿豆象成虫的LC50为6.979μL/L；硅藻土对绿豆象成虫体内过氧化氢酶、乙酰胆碱酯酶、琥珀酸脱氢酶、过氧化物酶、谷胱甘肽-S-转移酶和超氧化物歧化酶活性均无影响。表明茴香烯与硅藻土联合可用于绿色防控绿豆象。 中文关键词:绿豆象  茴香烯  硅藻土  熏蒸  酶活性   Screening of plant volatiles and their combined effects with diatomaceous earth on control efficacy and enzyme activities in Chinese bean weeril Callosobruchus chinensis Author Name Affiliation E-mail Li Zipeng  College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Li Huiqian  College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Yuan Xiaodan  College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Wu Anqi  College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Zhang Xianhong  College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    Zhang Zeyan  College of Agriculture, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China  447401080@qq.com  Zheng Haixia  College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China  zhenghaixia722@163.com  Abstract:To identify an environmentally friendly and efficient method for controlling Chinese bean weeril Callosobruchus chinensis, a sealed fumigation method was used to evaluate the fumigation effects of four plant volatiles（trans-anethole, citral, 4-allylanisole, and eugenol） on adult C. chinensis. The plant volatile with the best fumigation effect was selected and combined with diatomaceous earth to determine its laboratory toxicity, control efficacy, and effects on multiple enzyme activities in C. chinensis. The results showed that among the four plant volatiles, trans-anethole exhibited the strongest fumigation effect. At a concentration of 5 μL/L, trans-anethole achieved a corrected mortality rate of 100.00% against for adult C. chinensis after 48 hours of treatment. The combination of trans-anethole and diatomaceous earth demonstrated a significant synergistic effect, with an LC50 of 3.040 μL/L against adult C. chinensis, compared to 6.979 μL/L for trans-anethole alone. Diatomaceous earth alone had no effect on the activities of catalase, acetylcholinesterase, succinate dehydrogenase, peroxidase, glutathione-S-transferase, and superoxide dismutase in C. chinensis. These findings indicate that the combination of trans-anethole and diatomaceous earth can be used for the green control of C. chinensis. keywords:Callosobruchus chinensis  trans-anethole  diatomaceous earth  fumigation  enzymatic activity View Full Text  View/Add Comment  Download reader

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