稻瘟病菌内切几丁质酶基因MoENCH1的功能分析 Click here to download the full text Citation：赵娟,许立鹤,石鑫怡,邹欣梅,吕柔柔,赵鑫,刘杰.稻瘟病菌内切几丁质酶基因MoENCH1的功能分析.Journal of Plant Protection,2026,53(4):938-946 Hits: Download times: Author Name Affiliation E-mail Zhao Juan  Sichuan Provincial Key Laboratory for Development and Utilization of Characteristic Horticultural Biological Resources, Chengdu Normal University, Chengdu 611130, Sichuan Province, China  zhaojuan201809@163.com  Xu Lihe  Chengdu Agricultural College, Chengdu 611130, Sichuan Province, China    Shi Xinyi  Sichuan Provincial Key Laboratory for Development and Utilization of Characteristic Horticultural Biological Resources, Chengdu Normal University, Chengdu 611130, Sichuan Province, China    Zou Xinmei  Sichuan Provincial Key Laboratory for Development and Utilization of Characteristic Horticultural Biological Resources, Chengdu Normal University, Chengdu 611130, Sichuan Province, China    Lü Rourou  Sichuan Provincial Key Laboratory for Development and Utilization of Characteristic Horticultural Biological Resources, Chengdu Normal University, Chengdu 611130, Sichuan Province, China    Zhao Xin  Sichuan Provincial Key Laboratory for Development and Utilization of Characteristic Horticultural Biological Resources, Chengdu Normal University, Chengdu 611130, Sichuan Province, China    Liu Jie  Sichuan Provincial Key Laboratory for Development and Utilization of Characteristic Horticultural Biological Resources, Chengdu Normal University, Chengdu 611130, Sichuan Province, China    中文摘要:为明确稻瘟病菌Magnaporthe oryzae几丁质内切酶（endochitinase，ENCH）的功能，通过Splitmarker方法在稻瘟病菌中敲除编码该酶的MoENCH1基因获得敲除突变体，并构建回补菌株，系统分析其生物学功能，进一步采用转录组测序技术对其致病分子机制进行分析。结果表明：相较于野生型稻瘟病菌，MoENCH1基因敲除突变体在番茄燕麦（oatmeal agar，OMA）培养基、复杂完全培养基（complete medium，CM）和基本培养基（minimal medium，MM）上的生长速率均显著下降，在OMA培养基上的产孢量显著下降，孢子萌发率和附着胞形成率也均显著下降，且对细胞壁胁迫因子刚果红不敏感，对大麦和水稻的致病力显著下降。转录组分析结果显示，稻瘟病菌体内MoENCH1基因参与调控自噬、过氧化物酶体、泛素介导的蛋白酶解和甘油磷脂代谢通路。表明MoENCH1基因在稻瘟菌体内参与调控菌丝生长、产孢、孢子萌发、附着胞形成以及致病力。 中文关键词:稻瘟病菌  几丁质内切酶  致病力  转录组  产孢  孢子萌发  附着胞形成   Functional analysis of the endochitinase gene MoENCH1 in rice blast fungus Magnaporthe oryzae Author Name Affiliation E-mail Zhao Juan  Sichuan Provincial Key Laboratory for Development and Utilization of Characteristic Horticultural Biological Resources, Chengdu Normal University, Chengdu 611130, Sichuan Province, China  zhaojuan201809@163.com  Xu Lihe  Chengdu Agricultural College, Chengdu 611130, Sichuan Province, China    Shi Xinyi  Sichuan Provincial Key Laboratory for Development and Utilization of Characteristic Horticultural Biological Resources, Chengdu Normal University, Chengdu 611130, Sichuan Province, China    Zou Xinmei  Sichuan Provincial Key Laboratory for Development and Utilization of Characteristic Horticultural Biological Resources, Chengdu Normal University, Chengdu 611130, Sichuan Province, China    Lü Rourou  Sichuan Provincial Key Laboratory for Development and Utilization of Characteristic Horticultural Biological Resources, Chengdu Normal University, Chengdu 611130, Sichuan Province, China    Zhao Xin  Sichuan Provincial Key Laboratory for Development and Utilization of Characteristic Horticultural Biological Resources, Chengdu Normal University, Chengdu 611130, Sichuan Province, China    Liu Jie  Sichuan Provincial Key Laboratory for Development and Utilization of Characteristic Horticultural Biological Resources, Chengdu Normal University, Chengdu 611130, Sichuan Province, China    Abstract:To investigate the function of the endochitinase (ENCH) gene MoENCH1 in rice blast fungus Magnaporthe oryzae, a MoENCH1 knockout mutant was generated using the split-marker approach, and a complemented strain was subsequently constructed. The biological functions of MoENCH1 were systematically characterized, and the molecular mechanisms underlying its role in pathogenicity were further investigated with transcriptome sequencing. The results showed that, compared with the wildtype strain, the MoENCH1 knockout mutant significantly reduced growth rates on oatmeal agar (OMA), complete medium, and minimal medium. Sporulation on OMA was significantly decreased, accompanied by reduced conidial germination and appressorium formation rates. In addition, the mutant was insensitive to the cell wall stress agent Congo red and exhibited significantly reduced pathogenicity toward both barley and rice. Transcriptome analysis indicated that MoENCH1 is involved in regulating pathways related to autophagy, peroxisomes, ubiquitin-mediated proteolysis, and glycerophospholipid metabolism in M. oryzae. These results demonstrate that MoENCH1 plays important roles in regulating hyphal growth, sporulation, conidial germination, appressorium formation, and pathogenicity in M. oryzae. keywords:Magnaporthe oryzae  endochitinase  pathogenicty  RNA-Seq  sporulation  conidial germination  appressorium formation View Full Text  View/Add Comment  Download reader

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