甘肃省定西市玉米坏死条纹病毒分离物的分子鉴定及全基因组序列分析 Click here to download the full text Citation：房静静,胡小平,马悦,徐亨昊,张树武,徐秉良,薛应钰,梁巧兰,牛二波,王嘉荟.甘肃省定西市玉米坏死条纹病毒分离物的分子鉴定及全基因组序列分析.Journal of Plant Protection,2026,53(4):947-954 Hits: Download times: Author Name Affiliation E-mail Fang Jingjing  College of Plant Protection, Gansu Agricultural University, Anning 730070, Gansu Province, China    Hu Xiaoping  College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China    Ma Yue  College of Plant Protection, Gansu Agricultural University, Anning 730070, Gansu Province, China    Xu Henghao  College of Plant Protection, Gansu Agricultural University, Anning 730070, Gansu Province, China    Zhang Shuwu  College of Plant Protection, Gansu Agricultural University, Anning 730070, Gansu Province, China    Xu Bingliang  College of Plant Protection, Gansu Agricultural University, Anning 730070, Gansu Province, China    Xue Yingyu  College of Plant Protection, Gansu Agricultural University, Anning 730070, Gansu Province, China    Liang Qiaolan  College of Plant Protection, Gansu Agricultural University, Anning 730070, Gansu Province, China    Niu Erbo  College of Plant Protection, Gansu Agricultural University, Anning 730070, Gansu Province, China  13907@gsau.edu.cn  Wang Jiahui  College of Plant Protection, Gansu Agricultural University, Anning 730070, Gansu Province, China  13453295106@163.com  中文摘要:为鉴定甘肃省定西市导致玉米表现褪绿条纹症状的病原，利用高通量测序结合反转录PCR和5'/3'RACE技术对病原进行鉴定并解析其基因组序列，采用NCBI BLAST、MegAlign和IQTREE软件开展序列特征分析与系统发育树构建，并通过反转录PCR调查该病原在甘肃省不同地区的发生分布。结果表明：在定西市玉米病样中分离鉴定到玉米坏死条纹病毒（maize necroticstreak virus，MNeSV），将该分离物命名为MNeSV-DX，经序列拼接获得全长4 094 nt的基因组，包含5个开放阅读框，其基因组结构与两个MNeSV美国分离物（登录号分别为AF266518.2和NC_007729.1）一致，全基因组核苷酸和氨基酸序列相似性均分别为98.8%和99.0%，系统发育分析显示三者聚类于同一分支，亲缘关系近，遗传差异较小。检测结果显示MNeSV已侵染甘肃省定西、甘南和陇南3个市州的玉米。表明MneSV在我国可自然侵染玉米，MNeSV与定西市玉米褪绿条纹症状存在相关性，应给于高度重视。 中文关键词:玉米  玉米坏死条纹病毒  病毒鉴定  进化分析  发生  分布   Molecular identification and complete genome sequence analysis of a maize necrotic streak virus isolate from Dingxi, Gansu Province, China Author Name Affiliation E-mail Fang Jingjing  College of Plant Protection, Gansu Agricultural University, Anning 730070, Gansu Province, China    Hu Xiaoping  College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China    Ma Yue  College of Plant Protection, Gansu Agricultural University, Anning 730070, Gansu Province, China    Xu Henghao  College of Plant Protection, Gansu Agricultural University, Anning 730070, Gansu Province, China    Zhang Shuwu  College of Plant Protection, Gansu Agricultural University, Anning 730070, Gansu Province, China    Xu Bingliang  College of Plant Protection, Gansu Agricultural University, Anning 730070, Gansu Province, China    Xue Yingyu  College of Plant Protection, Gansu Agricultural University, Anning 730070, Gansu Province, China    Liang Qiaolan  College of Plant Protection, Gansu Agricultural University, Anning 730070, Gansu Province, China    Niu Erbo  College of Plant Protection, Gansu Agricultural University, Anning 730070, Gansu Province, China  13907@gsau.edu.cn  Wang Jiahui  College of Plant Protection, Gansu Agricultural University, Anning 730070, Gansu Province, China  13453295106@163.com  Abstract:To identify the pathogen associated with chlorotic streak symptoms in maize (Zea mays) in Dingxi City, Gansu Province, high-throughput sequencing combined with reverse transcription-poly-merase chain reaction (RT-PCR) and 5'/3' rapid amplification of cDNA ends (5'/3' RACE) was used to identify the virus and determine its complete genome sequence. Sequence characteristics and phylogenetic relationships were analyzed using NCBI BLAST, MegAlign, and IQ-TREE. RT-PCR was further used to investigate the occurrence and distribution of the virus in different regions of Gansu Province. The results showed that maize necrotic streak virus (MNeSV) was identified from symptomatic maize samples collected in Dingxi City and the isolate was designated MNeSV-DX. The complete genome of MNeSV-DX was 4 094 nt in length and contained five predicted open reading frames. Its genomic organizationwas identical to that of the US isolates (GenBank accession numbers NC_007729.1 and AF266518.2). The complete genome shared 98.8% nucleotide sequence identity and 99.0% amino acid sequence identity with these reference isolates. Phylogenetic analysis demonstrated that the three isolates clustered within the same clade, indicating a close evolutionary relationship with limited genetic divergence. RT-PCR detection further demonstrated the presence of MNeSV in maize samples collected from Dingxi, Gannan, and Longnan in Gansu Province. These findings indicate that MNeSV can naturally infect maize in China and is closely associated with the chlorotic streak symptoms observed in Dingxi City, highlighting the need for increased attention to the occurrence and spread of this virus. keywords:maize  maize necrotic streak virus (MNeSV)  virus identification  phylogenetic analysis  occurrence  distribution View Full Text  View/Add Comment  Download reader

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