青稞抗条纹病相关SHSP基因的筛选及序列分析 Click here to download the full text Citation：张驰,胡倩,严文天,姚有华,吴昆仑,姚晓华.青稞抗条纹病相关SHSP基因的筛选及序列分析.Journal of Plant Protection,2026,53(4):969-978 Hits: Download times: Author Name Affiliation E-mail Zhang Chi  College of Agriculture and Forestry, Qinghai University, Xining 810016, Qinghai Province, China Laboratory of Germplasm Research and Utilization on Qinghai-Tibet Plateau, Qinghai Provincial Key Laboratory of Hulless Barley Genetic Breeding, National Center for Wheat Improvement, Qinghai Highland Barley Sub-Center, Xining 810016, Qinghai Province, China    Hu Qian  College of Agriculture and Forestry, Qinghai University, Xining 810016, Qinghai Province, China Laboratory of Germplasm Research and Utilization on Qinghai-Tibet Plateau, Qinghai Provincial Key Laboratory of Hulless Barley Genetic Breeding, National Center for Wheat Improvement, Qinghai Highland Barley Sub-Center, Xining 810016, Qinghai Province, China    Yan Wentian  College of Agriculture and Forestry, Qinghai University, Xining 810016, Qinghai Province, China Laboratory of Germplasm Research and Utilization on Qinghai-Tibet Plateau, Qinghai Provincial Key Laboratory of Hulless Barley Genetic Breeding, National Center for Wheat Improvement, Qinghai Highland Barley Sub-Center, Xining 810016, Qinghai Province, China    Yao Youhua  College of Agriculture and Forestry, Qinghai University, Xining 810016, Qinghai Province, China Laboratory of Germplasm Research and Utilization on Qinghai-Tibet Plateau, Qinghai Provincial Key Laboratory of Hulless Barley Genetic Breeding, National Center for Wheat Improvement, Qinghai Highland Barley Sub-Center, Xining 810016, Qinghai Province, China    Wu Kunlun  College of Agriculture and Forestry, Qinghai University, Xining 810016, Qinghai Province, China Laboratory of Germplasm Research and Utilization on Qinghai-Tibet Plateau, Qinghai Provincial Key Laboratory of Hulless Barley Genetic Breeding, National Center for Wheat Improvement, Qinghai Highland Barley Sub-Center, Xining 810016, Qinghai Province, China    Yao Xiaohua  College of Agriculture and Forestry, Qinghai University, Xining 810016, Qinghai Province, China Laboratory of Germplasm Research and Utilization on Qinghai-Tibet Plateau, Qinghai Provincial Key Laboratory of Hulless Barley Genetic Breeding, National Center for Wheat Improvement, Qinghai Highland Barley Sub-Center, Xining 810016, Qinghai Province, China  yaoyiaohua009@126.com  中文摘要:为筛选青稞Hordeum vulgare var.nudum抗条纹病相关小分子热激蛋白（small heat shock proteins，SHSP）基因，根据抗、感病品种转录组测序数据筛选差异表达基因，克隆HvnSHSP21基因，分析HvnSHSP21的序列特征及表达模式，解析其编码蛋白序列结构及系统进化关系，并通过实时荧光定量PCR检测条纹病胁迫下不同抗性青稞品种中HvnSHSP21的表达情况。结果显示：从抗性品种THIBAUT和易感品种Z1141中克隆获得582 nt的编码序列（coding sequence，CDS），编码具有193个氨基酸的稳定亲水性蛋白，两个品种的基因片段存在两个差异核苷酸，导致产生两个氨基酸差异，该蛋白具有α-晶状体结构域，无跨膜结构域和信号肽，定位于叶绿体。二级结构以无规则卷曲和α-螺旋为主，两个品种间存在差异。系统进化分析结果表明，抗性品种中的HvnSHSP21蛋白与大麦SHSP同源性达100.00%。实时荧光定量PCR分析结果表明，HvnSHSP基因受条纹病诱导表达，接种条纹病菌后，青稞不同抗性品种24-189、9726、22-732与0349-1中HvnSHSP基因的表达量均呈上调趋势，且抗性品种中该基因的表达量显著高于易感品种。 中文关键词:青稞  条纹病  转录组分析  SHSP  序列分析  表达分析   Screening and sequence analysis of SHSP genes associated with resistance to stripe disease in hulless barley Author Name Affiliation E-mail Zhang Chi  College of Agriculture and Forestry, Qinghai University, Xining 810016, Qinghai Province, China Laboratory of Germplasm Research and Utilization on Qinghai-Tibet Plateau, Qinghai Provincial Key Laboratory of Hulless Barley Genetic Breeding, National Center for Wheat Improvement, Qinghai Highland Barley Sub-Center, Xining 810016, Qinghai Province, China    Hu Qian  College of Agriculture and Forestry, Qinghai University, Xining 810016, Qinghai Province, China Laboratory of Germplasm Research and Utilization on Qinghai-Tibet Plateau, Qinghai Provincial Key Laboratory of Hulless Barley Genetic Breeding, National Center for Wheat Improvement, Qinghai Highland Barley Sub-Center, Xining 810016, Qinghai Province, China    Yan Wentian  College of Agriculture and Forestry, Qinghai University, Xining 810016, Qinghai Province, China Laboratory of Germplasm Research and Utilization on Qinghai-Tibet Plateau, Qinghai Provincial Key Laboratory of Hulless Barley Genetic Breeding, National Center for Wheat Improvement, Qinghai Highland Barley Sub-Center, Xining 810016, Qinghai Province, China    Yao Youhua  College of Agriculture and Forestry, Qinghai University, Xining 810016, Qinghai Province, China Laboratory of Germplasm Research and Utilization on Qinghai-Tibet Plateau, Qinghai Provincial Key Laboratory of Hulless Barley Genetic Breeding, National Center for Wheat Improvement, Qinghai Highland Barley Sub-Center, Xining 810016, Qinghai Province, China    Wu Kunlun  College of Agriculture and Forestry, Qinghai University, Xining 810016, Qinghai Province, China Laboratory of Germplasm Research and Utilization on Qinghai-Tibet Plateau, Qinghai Provincial Key Laboratory of Hulless Barley Genetic Breeding, National Center for Wheat Improvement, Qinghai Highland Barley Sub-Center, Xining 810016, Qinghai Province, China    Yao Xiaohua  College of Agriculture and Forestry, Qinghai University, Xining 810016, Qinghai Province, China Laboratory of Germplasm Research and Utilization on Qinghai-Tibet Plateau, Qinghai Provincial Key Laboratory of Hulless Barley Genetic Breeding, National Center for Wheat Improvement, Qinghai Highland Barley Sub-Center, Xining 810016, Qinghai Province, China  yaoyiaohua009@126.com  Abstract:To screen for small heat shock protein (SHSP) genes related to stripe disease resistance in hulless barley (Hordeum vulgare var. nudum), differentially expressed genes were screened based on transcriptome sequencing data of resistant and susceptible cultivars. The HvnSHSP21 gene was cloned, and its sequence characteristics and expression patterns were analyzed. The sequence structure and phylogenetic relationship of the encoded protein were examined, and its expression levels under stripe disease stress in different resistant cultivars were detected by quantitative real-time PCR. The results showed that an identical 582 nt coding sequence (CDS) was cloned from both the resistant cultivar THIBAUT and the susceptible cultivar Z1141, which encodes a stable hydrophilic protein consisting of 193 amino acids. Two nucleotide differences were found between the two cultivars, resulting in two amino acid changes. The protein contains an alpha-crystallin domain, lacks transmembrane domains and signal peptides, and is predicted to localize to the chloroplast. Its secondary structure is mainly composed of random coils and α-helices, with differences observed between the two cultivars. Phylogenetic analysis indicated that the HvnSHSP21 protein from the resistant cultivar shared 100.00% homology with barley SHSP. Quantitative real-time PCR analysis revealed that the HvnSHSP21 gene was induced by stripe disease stress. Following inoculation with Pyrenophora graminea, the transcript levels of HvnSHSP21 were significantly up-regulated in all four hulless barley cultivars (24-189, 9726, 22-732, and 0349-1).Notably, resistant cultivars exhibited significantly higher HvnSHSP21 expression than susceptible cultivars (P<0.05). keywords:hulless barley  stripe disease  RNA-seq analysis  SHSP  sequence analysis  expression analysis View Full Text  View/Add Comment  Download reader

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