贵州省烟区病毒多样性检测及辣椒脉斑驳病毒的流行特征与群体遗传分析 Click here to download the full text Citation：林涛,张翼飞,金鑫,曹毅,李婷婷,丁铭.贵州省烟区病毒多样性检测及辣椒脉斑驳病毒的流行特征与群体遗传分析.Journal of Plant Protection,2026,53(4):979-988 Hits: Download times: Author Name Affiliation E-mail Lin Tao  Key Laboratory of the Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture and Rural Affairs Yunnan Province Key Laboratory of Agricultural Biotechnology Institute of Biotechnology and Germplasm Resources, Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan Province, China    Zhang Yifei  Guiyang Company, Guizhou Tobacco Company, Guiyang 550004, Guizhou Province, China    Jin Xin  Guiyang Company, Guizhou Tobacco Company, Guiyang 550004, Guizhou Province, China    Cao Yi  Guizhou Academy of Tobacco Science, Guiyang 550081, Guizhou Province, China    Li Tingting  Key Laboratory of the Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture and Rural Affairs Yunnan Province Key Laboratory of Agricultural Biotechnology Institute of Biotechnology and Germplasm Resources, Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan Province, China  591807832@qq.com  Ding Ming  Key Laboratory of the Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture and Rural Affairs Yunnan Province Key Laboratory of Agricultural Biotechnology Institute of Biotechnology and Germplasm Resources, Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan Province, China  mingd73@163.com  中文摘要:为明确贵州省烟区病毒多样性及辣椒脉斑驳病毒（chilli veinal mottle virus，ChiVMV）的流行规律，于2025年8—10月在贵阳、安顺和兴义3市采集烟草Nicotiana tabacum及其伴生杂草样品共74份，通过宏转录组测序与反转录PCR进行病毒分析与检测，并通过衣壳蛋白（coat protein，CP）基因序列比对、SDT一致性分析及系统发育分析对ChiVMV进行遗传分析。结果显示：从采集样品中共鉴定出8种病毒，检出率排前4位的分别为烟草花叶病毒（tobacco mosaic virus，TMV）、烟草扭脉病毒（tobacco vein distorting virus，TVDV）、马铃薯Y病毒（potato virus Y，PVY）和ChiVMV，其中ChiVMV在贵阳市烟区烟草样品中的检出率达87.50%，且在安顺市烟草及兴义市育苗棚杂草中普遍存在，表明该病毒已在贵州省烟区广泛定殖。基于CP基因序列的系统发育分析显示，ChiVMV在以我国云贵川为核心的西南烟区及辽宁等省存在本地循环演化的遗传结构，同时还可能伴随与巴基斯坦、印度等南亚国家和地区之间的长距离扩散或引入事件。此外，兴义市育苗棚伴生杂草中ChiVMV的检出率达100.00%，说明这些伴生杂草是ChiVMV传播的重要初侵染源。表明ChiVMV在贵州省烟区的流行潜力与传播风险较高，应将监测病毒多样性、重点防控育苗环节伴生杂草毒源作为关注重点，从源头控制其传播扩散。 中文关键词:辣椒脉斑驳病毒  烟草  病毒多样性  CP基因  系统发育  杂草   Detection of viral diversity and epidemiological characteristics and population genetic analysis of chilli veinal mottle virus in tobacco-growing areas of Guizhou Province Author Name Affiliation E-mail Lin Tao  Key Laboratory of the Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture and Rural Affairs Yunnan Province Key Laboratory of Agricultural Biotechnology Institute of Biotechnology and Germplasm Resources, Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan Province, China    Zhang Yifei  Guiyang Company, Guizhou Tobacco Company, Guiyang 550004, Guizhou Province, China    Jin Xin  Guiyang Company, Guizhou Tobacco Company, Guiyang 550004, Guizhou Province, China    Cao Yi  Guizhou Academy of Tobacco Science, Guiyang 550081, Guizhou Province, China    Li Tingting  Key Laboratory of the Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture and Rural Affairs Yunnan Province Key Laboratory of Agricultural Biotechnology Institute of Biotechnology and Germplasm Resources, Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan Province, China  591807832@qq.com  Ding Ming  Key Laboratory of the Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture and Rural Affairs Yunnan Province Key Laboratory of Agricultural Biotechnology Institute of Biotechnology and Germplasm Resources, Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan Province, China  mingd73@163.com  Abstract:To clarify the viral diversity and the epidemiological patterns of chilli veinal mottle virus (ChiVMV) in tobacco-growing areas of Guizhou Province, a total of 74 samples of tobacco Nicotiana tabacum and associated weeds were collected from Guiyang, Anshun, and Xingyi cities between August and October 2025. Viruses were detected and analyzed using metatranscriptomic sequencing and reverse transcription-PCR. Genetic analysis of ChiVMV was performed based on the coat protein (CP) gene sequence alignment, SDT pairwise nucleotide identity analysis, and the construction of a neighborjoining phylogenetic tree. A total of eight viruses were identified in this research. The top three most frequently detected viruses were tobacco mosaic virus (TMV), tobacco vein distorting virus (TVDV), and potato virus Y (PVY) together with ChiVMV. Notably, the detection rate of ChiVMV in tobacco samples from Guiyang was as high as 87.50%, and the virus was also prevalent in tobacco from Anshun and in weeds from seedling nurseries in Xingyi, indicating that it has become widely established in Guizhou tobacco-growing regions. Phylogenetic analysis based on the CP gene revealed that ChiVMV isolates exhibited a genetic structure of local endemic circulation in southwestern China (centered in Yunnan, Guizhou, and Sichuan provinces) as well as in Liaoning Province, and might have undergone long-distance dispersal or introduction events involving South Asian regions such as Pakistan and India. Furthermore, the detection rate of ChiVMV in associated weeds from Xingyi seedling nurseries reached 100.00%, suggesting that these weeds serve as an important primary inoculum source for virus transmission. These findings indicate a high epidemic potential and transmission risk of ChiVMV in Guizhou tobacco-growing areas. Therefore, it is critical to monitor virus diversity and focus on controlling weedbased virus sources during the seedling nursery stage, thereby curbing its spread from the source. keywords:chilli veinal mottle virus  tobacco  virus diversity  CP gene  phylogenetic analysis  weed View Full Text  View/Add Comment  Download reader

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