黄暗色链霉菌A15的广谱抑菌活性、发酵条件优化及对植物的促生作用 Click here to download the full text Citation：李光哲,杨晶婷,黄但,朱峰,王平平,颜霞,杨明明,黄丽丽.黄暗色链霉菌A15的广谱抑菌活性、发酵条件优化及对植物的促生作用.Journal of Plant Protection,2026,53(4):1019-1032 Hits: Download times: Author Name Affiliation E-mail Li Guangzhe  State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Northwest A&F University, Yangling 712100, Shaanxi Province, China College of Life Sciences, Northwest A&F University, Yangling 712100, Shaanxi Province, China    Yang Jingting  State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Northwest A&F University, Yangling 712100, Shaanxi Province, China College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China    Huang Dan  State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Northwest A&F University, Yangling 712100, Shaanxi Province, China College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China    Zhu Feng  Ankang Branch of Shaanxi Tobacco Company, Ankang 725000, Shaanxi Province, China    Wang Pingping  Shaanxi Provincial Tobacco Corporation of China National Tobacco Corporation, Xi'an 710061, Shaanxi Province, China    Yan Xia  State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Northwest A&F University, Yangling 712100, Shaanxi Province, China College of Life Sciences, Northwest A&F University, Yangling 712100, Shaanxi Province, China    Yang Mingming  State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Northwest A&F University, Yangling 712100, Shaanxi Province, China College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China  yangming862@126.com  Huang Lili  State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Northwest A&F University, Yangling 712100, Shaanxi Province, China College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China  huanglili@nwsuaf.edu.cn  中文摘要:为明确本实验室前期获得的菌株A15的生防潜力，采用皿内抑菌法测定其对丁香假单胞菌烟草致病变种Pseudomonas syringae pv.tabaci的抑制效果，通过形态学观察和16S rRNA-atpDgyrB-recA-rpoB多基因系统发育分析对其进行鉴定，采用单因素试验和正交试验对其发酵条件进行优化，并对其发酵液无菌滤液的抑菌谱、离体防效及促生能力进行测定。结果表明：菌株A15对丁香假单胞菌烟草致病变种具有显著拮抗效果，其抑菌圈直径为1.86 cm，经鉴定该菌株为黄暗色链霉菌Streptomyces xanthophaeus。该菌株最佳发酵培养基配方为可溶性淀粉10 g/L、酵母提取物10 g/L、NaCl 15 g/L。菌株A15的发酵液无菌滤液对多种病原细菌和真菌均表现出较好的拮抗效果，且对烟草野火病、苹果树腐烂病和猕猴桃细菌性溃疡病具有较好的防治效果。此外，菌株A15能产生吲哚乙酸和嗜铁素，其发酵液具有促进烟草幼苗根长和地上部分干物质积累的效果。综上所述，菌株A15具有良好的生防潜力，可作为多种植物病害生物防治的备选菌种资源。 中文关键词:黄暗色链霉菌  发酵条件优化  生物防治  促生能力  鉴定  抑菌谱   Broad-spectrum antimicrobial activity, fermentation optimization and plant growth-promoting effects of Streptomyces xanthophaeus strain A15 Author Name Affiliation E-mail Li Guangzhe  State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Northwest A&F University, Yangling 712100, Shaanxi Province, China College of Life Sciences, Northwest A&F University, Yangling 712100, Shaanxi Province, China    Yang Jingting  State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Northwest A&F University, Yangling 712100, Shaanxi Province, China College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China    Huang Dan  State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Northwest A&F University, Yangling 712100, Shaanxi Province, China College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China    Zhu Feng  Ankang Branch of Shaanxi Tobacco Company, Ankang 725000, Shaanxi Province, China    Wang Pingping  Shaanxi Provincial Tobacco Corporation of China National Tobacco Corporation, Xi'an 710061, Shaanxi Province, China    Yan Xia  State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Northwest A&F University, Yangling 712100, Shaanxi Province, China College of Life Sciences, Northwest A&F University, Yangling 712100, Shaanxi Province, China    Yang Mingming  State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Northwest A&F University, Yangling 712100, Shaanxi Province, China College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China  yangming862@126.com  Huang Lili  State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Northwest A&F University, Yangling 712100, Shaanxi Province, China College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China  huanglili@nwsuaf.edu.cn  Abstract:To evaluate the biocontrol potential of bacterium strain A15 previously isolated in our laboratory, its antagonistic activity against Pseudomonas syringae pv. tabaci was assessed using an in vitro antagonism assay. The strain was identified based on morphological characteristics and multilocus phylogenetic analysis of the 16S rRNA, atpD, gyrB, recA and rpoB genes. Fermentation conditions were optimized using single-factor experiments and an orthogonal experimental design. The antimicrobial spectrum, ex vivo biocontrol efficacy, and plant growth-promoting ability of the sterile filtrate from the fermentation broth were subsequently evaluated. The results showed that strain A15 exhibited significant antagonistic activity against P. syringae pv. tabaci, with an inhibition zone diameter of 1.86 cm. The strain was identified as Streptomyces xanthophaeus. The optimal fermentation medium consisted of 10 g/L soluble starch, 10 g/L yeast extract, and 15 g/L NaCl. The sterile filtrate of the fermentation broth exhibited broad-spectrum antagonistic activity against multiple bacterial and fungal pathogens and showed good control efficacy against tobacco wildfire disease, apple Valsa canker, and kiwifruit bacterial canker. Additionally, strain A15 produced indole-3-acetic acid (IAA) and siderophores, and its fermentation broth promoted tobacco seedling root elongation and shoot dry matter accumulation. In conclusion, strain A15 possesses considerable biocontrol potential and may serve as a promising microbial resource for the biological control of multiple plant diseases. keywords:Streptomyces xanthophaeus  fermentation optimization  biological control  plant growthpromoting activity  identification  antimicrobial spectrum View Full Text  View/Add Comment  Download reader

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