甲硫氨酸修饰聚琥珀酰亚胺纳米戊唑醇的传导释放及对山药茎腐病的防效 Click here to download the full text Citation：刘国鑫,唐博文,侠天琦,冯晓晓,赵斌,刘颖超.甲硫氨酸修饰聚琥珀酰亚胺纳米戊唑醇的传导释放及对山药茎腐病的防效.Journal of Plant Protection,2026,53(4):1062-1072 Hits: Download times: Author Name Affiliation E-mail Liu Guoxin  College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China    Tang Bowen  College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China    Xie Tianqi  College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China    Feng Xiaoxiao  College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China    Zhao Bin  College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China    Liu Yingchao  College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China  liuyingchao@hebau.edu.cn  中文摘要:为解决山药茎腐病防治中传统药剂难以传导至根部的问题，用甲硫氨酸修饰聚琥珀酰亚胺纳米载体（polysuccinimide modified with methionine nanocarrier，PMA）负载戊唑醇（tebuconazole，Teb）构建pH响应型甲硫氨酸修饰聚琥珀酰亚胺纳米戊唑醇（tebuconazole-loaded polysuccinimide modified with methionine nanocarrier，Teb@PMA），采用核磁共振氢谱、傅里叶变换红外光谱、动态光散射、扫描电子显微镜等技术对纳米载体PMA和纳米农药Teb@PMA的结构和形态进行表征，并分析不同pH下纳米农药Teb@PMA的释放率、在叶片表面的接触角、耐冲刷能力、在植株体内的分布及对两种镰孢菌的生物活性和防效。结果显示：纳米农药Teb@PMA在pH 8.5条件下72 h累计释放率为89.23%；在叶片上的接触角比悬浮剂减小22.11%，滞留率为41.41%；该制剂具有良好的双向传导能力，施药后14 d在中部叶片和根中戊唑醇浓度分别为0.306 mg/kg和0.020 mg/kg，显著高于悬浮剂处理。纳米农药Teb@PMA对尖孢镰孢菌Fusarium oxysporum的有效中浓度EC50为3.168 mg/L，对山药茎腐病的治疗防效为52.46%，较戊唑醇悬浮剂的治疗防效显著提高。表明纳米农药Teb@PMA显著提升了戊唑醇在植株体内的双向传导能力和对尖孢镰孢菌的活性，在防控土传病害方面具有潜力。 中文关键词:山药茎腐病  纳米农药  戊唑醇  pH响应  释放  双向传导   Bidirectional translocation and release of tebuconazole-loaded olysuccinimide modified with methionine nanocarrier for controlling yam stem rot Author Name Affiliation E-mail Liu Guoxin  College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China    Tang Bowen  College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China    Xie Tianqi  College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China    Feng Xiaoxiao  College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China    Zhao Bin  College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China    Liu Yingchao  College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China  liuyingchao@hebau.edu.cn  Abstract:To address the challenge of poor root translocation of conventional fungicides for yam stem rot control, a pH-responsive methionine-modified polysuccinimide nanocarrier system loaded with tebuconazole (Teb@PMA) was constructed using encapsulating tebuconazole (Teb) within methioninemodified polysuccinimide (PMA) nanocarriers. The structures and morphologies of PMA and Teb@PMA were characterized by proton nuclear magnetic resonance, Fourier transform infrared spectroscopy, dynamic light scattering, and scanning electron microscopy. The pH-dependent release profiles, leaf surface wettability, rainfastness, plant tissue distribution, and bioactivities against two Fusarium species were systematically investigated. The results showed that the cumulative release rate reached 89.23% at 72 h under simulated phloem alkaline conditions (pH 8.5). Compared with tebuconazole suspension concentrate, Teb@PMA exhibited a 22.11% reduction in contact angle on leaf surfaces and an increased retention rate of 41.41%. Moreover, Teb@PMA exhibited excellent bidirectional translocation. At 14 days after application, the tebuconazole content in middle leaves and roots was 0.306 mg/kg and 0.020 mg/kg, respectively, significantly higher than those of the suspension concentrate treatment. The in vitro toxicity assay showed that the EC50 of Teb@PMA against Fusarium oxysporum was 3.168 mg/L, and the in vitro test demonstrated that its curative efficacy against yam stem rot was 52.46%, significantly higher than that of the suspension concentrate (18.03%). Collectively, these findings indicate that the PMA nanocarrier delivery system substantially improves the bidirectional translocation of tebuconazole within plants and its activity against F. oxysporum, showing promising potential for the management of soil-borne diseases. keywords:yam stem rot  nanopesticide  tebuconazole  pH-response  release  bidirectional translocation View Full Text  View/Add Comment  Download reader

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