异色瓢虫Scalloped基因鉴定及其对翅发育的影响 Click here to download the full text Citation：张伟伟,周旬,尹嘉怡,卫小琳,张彬,石岩.异色瓢虫Scalloped基因鉴定及其对翅发育的影响.Journal of Plant Protection,2026,53(4):1073-1084 Hits: Download times: Author Name Affiliation E-mail Zhang Weiwei  College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, Shandong Province, China    Zhou Xun  College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, Shandong Province, China    Yin Jiayi  College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, Shandong Province, China    Wei Xiaolin  College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, Shandong Province, China    Zhang Bin  College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, Shandong Province, China  shiyanyuanyi@qau.edu.cn  Shi Yan  College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, Shandong Province, China  binzhang@qau.edu.cn  中文摘要:为解析天敌昆虫异色瓢虫Harmonia axyridis翅发育的分子机制，克隆异色瓢虫Hippo信号通路核心因子Scalloped同源基因，对其进行生物信息学分析，测定其在异色瓢虫不同发育阶段中的相对表达量，分析沉默Scalloped基因对异色瓢虫存活、羽化、成虫前后翅周长及翅畸形的影响，并于显微镜下观察沉默Scalloped基因后异色瓢虫翅基肌肉微观组织结构变化。结果表明：异色瓢虫Scalloped蛋白含高度保守TEA、YAP功能结构域；Scalloped的氨基酸序列与瓢虫科近缘物种的氨基酸序列亲缘关系较近、基因排布模式高度一致；在异色瓢虫不同发育阶段Scalloped基因均表达，其中在成虫期的相对表达量最高，卵期次之；异色瓢虫3龄幼虫注射dsScalloped后，抑制作用可持续至幼虫期、蛹期和成虫期，且最优剂量为每头注射80 ng；随dsScalloped注射剂量升高，异色瓢虫成虫翅畸形逐级加重，当每头注射80 ng dsScalloped后成虫前后翅周长均较对照极显著降低，飞行能力彻底丧失，蛹体翅基皱缩畸变，蛹翅基肌纤维破碎、肌细胞排布紊乱。表明Scalloped是调控异色瓢虫翅形态、飞行肌发育及变态蜕皮的关键基因。 中文关键词:异色瓢虫  Scalloped  RNA干扰  翅发育  Hippo通路  石蜡切片   Identification of the Scalloped gene in multicolored Asian lady beetle Harmonia axyridis and its role in wing development Author Name Affiliation E-mail Zhang Weiwei  College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, Shandong Province, China    Zhou Xun  College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, Shandong Province, China    Yin Jiayi  College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, Shandong Province, China    Wei Xiaolin  College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, Shandong Province, China    Zhang Bin  College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, Shandong Province, China  shiyanyuanyi@qau.edu.cn  Shi Yan  College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, Shandong Province, China  binzhang@qau.edu.cn  Abstract:To elucidate the molecular mechanism underlying wing development in the natural enemy insect Harmonia axyridis, the homolog of Scalloped, a core component of the Hippo signaling pathway, was cloned and subjected to bioinformatic analysis. Its relative expression levels at different developmental stages, were determined, and the effects of Scalloped silencing on survival, adult emergence, forewing and hindwing perimeters and wing malformation were investigated. In addition, microscopic observations were performed to examine changes in the microstructure of wing-base muscles following Scalloped silencing. The results showed that the Scalloped protein contains highly conserved TEA and YAP functional domains. The amino acid sequence of Scalloped from H. axyridis showed high similarity to those of closely related coccinellid species, and the gene organization was highly conserved. Scalloped was expressed throughout all developmental stages of H. axyridis, with the highest relative expression in adults, followed by eggs, Injection of dsScalloped into the 3rd-instar larvae resulted in persisted gene silencing throughout the larval, pupal and adult stages, with an optimal dose of 80 ng per individual. Increasing doses of dsScalloped caused progressively more severe, wing malformation. At a dose of 80 ng per individual, both forewing and hindwing perimeters were significantly lower than those of the control group, flight capacity was completely lost, the pupal wing bases exhibited shrinkage and deformation, and fractured muscle fibers together with disorganized muscle cell arrangement were observed at the wing bases. These results demonstrate that Scalloped is a key regulator of wing morphogenesis, flight muscle development and metamorphosis in H. axyridis. keywords:Harmonia axyridis  Scalloped  RNA interference  wing development  Hippo signaling pathway  paraffin section View Full Text  View/Add Comment  Download reader

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