爪哇虫草菌CJ01的分离鉴定及其对烟粉虱的控害效果评价 Click here to download the full text Citation：王乔弟,阳良旭,林威,黄国华.爪哇虫草菌CJ01的分离鉴定及其对烟粉虱的控害效果评价.Journal of Plant Protection,2026,53(4):1096-1104 Hits: Download times: Author Name Affiliation E-mail Wang Qiaodi  Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha 410128, Hunan Province, China Yuelushan Laboratory, Changsha 410128, Hunan Province, China    Yang Liangxu  Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha 410128, Hunan Province, China Yuelushan Laboratory, Changsha 410128, Hunan Province, China    Lin Wei  Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha 410128, Hunan Province, China Yuelushan Laboratory, Changsha 410128, Hunan Province, China    Huang Guohua  Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha 410128, Hunan Province, China Yuelushan Laboratory, Changsha 410128, Hunan Province, China  ghhuang@hunau.edu.cn  中文摘要:为筛选高效的烟粉虱Bemisia tabaci生防菌株，从其僵虫中分离虫生真菌，结合形态学特征和ITS序列分析对其进行鉴定，系统测定其生物学特性，并采用喷雾法测定其对烟粉虱成虫的毒力及田间防效。结果表明：从烟粉虱僵虫中分离获得1株虫生真菌菌株，经鉴定为爪哇虫草菌Cordyceps javanicai，命名为CJ01。该菌株在28℃、pH 7.0、24 h全光照条件下生长良好，使用麦芽提取物培养基培养时，其产孢量和生长速度均较高，培养至第7天产孢量为2.38×108个/皿。在室内，当该菌株孢子悬浮液浓度为1.0×108个/mL时，处理后3 d烟粉虱成虫累计校正死亡率可达83.93%；LC50为5.50×106个/mL。该菌株在田间试验中控害效果优异，施药14 d后对茄子田中烟粉虱的防效可达68.56%。表明爪哇虫草菌CJ01对烟粉虱的毒力较好，具有进一步研究和开发为生防制剂的潜力。 中文关键词:爪哇虫草菌  烟粉虱  致病力  田间试验  鉴定  生物学特性   Isolation and identification of Cordyceps javanicai strain CJ01 and evaluation of its biocontrol efficacy against tobacco whitefly Bemisia tabaci Author Name Affiliation E-mail Wang Qiaodi  Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha 410128, Hunan Province, China Yuelushan Laboratory, Changsha 410128, Hunan Province, China    Yang Liangxu  Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha 410128, Hunan Province, China Yuelushan Laboratory, Changsha 410128, Hunan Province, China    Lin Wei  Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha 410128, Hunan Province, China Yuelushan Laboratory, Changsha 410128, Hunan Province, China    Huang Guohua  Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha 410128, Hunan Province, China Yuelushan Laboratory, Changsha 410128, Hunan Province, China  ghhuang@hunau.edu.cn  Abstract:To identify an effective biocontrol agent against tobacco whitefly Bemisia tabaci, an entomopathogenic fungus was isolated and identified from naturally infected whitefly cadavers. The isolate was identified based on morphological characteristics and ITS sequence analysis. Its biological characteristics were systematically evaluated, and its virulence against B. tabaci adults and field control efficacy were determined using a spray bioassay. The results showed that a single entomopathogenic fungal isolate was obtained and identified as Cordyceps javanicai, designated CJ01. The strain grew well at 28 ℃, pH 7.0, under continuous light (24 h), and exhibited relatively high sporulation and growth rate on malt extract agar (MEA). The spore yield reached 2.38×108 conidia/plate after 7 days of cultivation. Under laboratory conditions, treatment with a spore suspension of 1.0×108 conidia/mL resulted in a cumulative corrected mortality of 83.93% in adult B. tabaci at 3 days after treatment, with an LC50 of 5.50×106 conidia/mL. In field trials, the strain showed excellent control efficacy against B. tabaci on eggplant, reaching 68.56% at 14 days after application. These results indicate that C. javanica CJ01 possesses strong virulence against B. tabaci and has considerable potential for further development as a microbial biocontrol agent. keywords:Cordyceps javanicai  Bemisia tabaci  virulence  field trial  identification  biological characteristics View Full Text  View/Add Comment  Download reader

Copyright:    京ICP备05006550号-2  You are the first  971344  Visitors
Head of Unit：Responsible Institution China Association for Scienc Organizers：China Society of Plant Protection and China Agricultural University Address：College of Agronomy and Biotechnology, China Agricultural University
Phone：86 (10) 62732528 E-mail：zbxb@cau.edu.cn
Technical Support：Tiller Beijing Science and Technology Development Co., Ltd.